References

380 E. Wagner et al.
Fig.25.7. Pattern of AK isozymes from C. rubrum (ecotype 374) after chromatography
on DEAE-cellulose. Seedlings grown for 4.5 days at 12 h : 12 h 32.5 ◦ C:10 ◦ C and 6,500 lx
continuous white light on filter paper with H2O; thereafter on Hoagland’s solution at 20 ◦ C
for 1 day followed by 5.5 days on 0.2 M glucose in Hoagland’s solution. The abundance
of the different isozymes in the cellular compartments changes after glucose application
as compared with that in untreated plants (c.f. vegetative pattern as a control, Fig. 25.6).
There is an increase of AK II and AK III abundance in the cytoplasm and the chloroplasts,
respectively. AK IV (nucleus) was not detected (Wagner et al. 1983)
concluded that the change of aquaporin expression at the apical meristem
during floral transition could be responsible for increased water movement
into the meristem provoking its expansion. Further studies should reveal if
intracellular pH and calcium concentration can influence water transport
by regulating CrAQP activity (Lopez et al. 2003).
25.7
Electrophysiological Integration of Activity
of the Whole Plant – Monitoring of Surface Sum Potentials
Automatic measurements of up to 4 weeks’ duration could be performed
using a measuring system with surface electrodes for the recording of
surface sum potentials. The following measuring procedures were used
(Wagner et al. 2004).