Scientific and Technical Aerospace Reports Volume 39 April 6, 2001
Scientific and Technical Aerospace Reports Volume 39 April 6, 2001
Scientific and Technical Aerospace Reports Volume 39 April 6, 2001
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non-steroidal activators of the receptors may act in a cell type <strong>and</strong> promoter dependent manner as we do not detect receptor activation<br />
by growth factors in our system.<br />
DTIC<br />
Estrogens; Mammary Gl<strong>and</strong>s; Cells (Biology); Steroids; Hormones; Culture Techniques<br />
<strong>2001</strong>0022469 Weizmann Inst. of Science, Rehovot, Israel<br />
X-ray Crystallographic Studies on Acetylcholinesterase <strong>and</strong> Related Enzymes Annual Report, 15 Sep. 1999 - 14 Sep. 2000<br />
Sussman, Joel L., Weizmann Inst. of Science, Israel; Silman, Israel, Weizmann Inst. of Science, Israel; October 2000; 382p; In<br />
English<br />
Contract(s)/Grant(s): DAMD17-97-2-7022<br />
Report No.(s): AD-A385536; No Copyright; Avail: CASI; A03, Microfiche; A17, Hardcopy<br />
The 3D structures are reported of complexes of conjugates of TcAChE with the nerve agents sarin, soman, DFP, VX <strong>and</strong> tabun.<br />
3D structures are also reported of complexes of AChE with the anti-Alzheimer drugs E2020, galanthamine, <strong>and</strong> huperzine A <strong>and</strong><br />
analogs. Time-resolved crystallographic studies are described aimed at elucidating routes of traffic of substrates <strong>and</strong> products.<br />
Specific chemical damage to proteins produced by synchrotron radiation is demonstrated for TcAChE <strong>and</strong> hen egg white lysozyme.<br />
The effects of site directed mutagenesis in the peripheral site on the stability of TcAChE are described. Buried <strong>and</strong> activesite<br />
water molecules of TcAChE were analyzed. Three different crystal forms of TcAChE were crystallized <strong>and</strong> analyzed. The<br />
quaternary structure of the electric eel tetramer was determined. The 3D structures of recombinant human AChE complexed with<br />
fasiculin-II <strong>and</strong> of recombinant Drosophila AChE were solved. Homology modeling of AChE <strong>and</strong> related alpha/beta hydrolase<br />
fold proteins was performed. A theoretical analysis of molecular traffic through ChEs was carried out. Molecular mechanical <strong>and</strong><br />
quantum analyses of cation-pi <strong>and</strong> aromatic-aromatic interactions were made. Enzyme deglycosylation of various ChE preparations<br />
were studied. A baculovirus expression system for human paraoxonase was developed.<br />
DTIC<br />
Crystallography; Chemical Defense; X Rays; Cholinesterase; Acetyl Compounds<br />
<strong>2001</strong>0022471 V<strong>and</strong>erbilt Univ., Nashville, TN USA<br />
Genetic Analysis of a Mammalian Chromosomal Origin of Replication Annual Report, 1 Aug. 1999 - 31 Jul. 2000<br />
Altman, Amy Lynn, V<strong>and</strong>erbilt Univ., USA; Fanning, Ellen, V<strong>and</strong>erbilt Univ., USA; August 2000; 65p; In English<br />
Contract(s)/Grant(s): DAMD17-99-1-9420<br />
Report No.(s): AD-A385<strong>39</strong>1; No Copyright; Avail: CASI; A01, Microfiche; A04, Hardcopy<br />
To identify cis-acting genetic elements for mammalian chromosomal DNA replication, a 5.7 kb fragment from the Chinese<br />
hamster dihydrofolate reductase (DHFR) locus containing the ori-beta initiation region was stably transfected into r<strong>and</strong>om ectopic<br />
chromosomal locations in a hamster cell line lacking the endogenous DHFR locus. Initiation at ectopic ori-beta in uncloned pools<br />
of transfected cells was measured using a competitive PCR-based nascent str<strong>and</strong> abundance assay <strong>and</strong> shown to mimic that at the<br />
endogenous ori-beta in CHOK1 hamster cells. Initiation activity of three ectopic ori-beta deletion mutants was reduced, while<br />
the activity of another deletion mutant was enhanced. These results suggest that a 5.7 kb fragment of DHFR ori-beta is sufficient<br />
to direct initiation <strong>and</strong> that specific DNA sequences in ori-beta are required for efficient initiation activity.<br />
DTIC<br />
Chromosomes; Genetics; Deoxyribonucleic Acid; Mammals; Assaying<br />
<strong>2001</strong>0022803 Battelle Memorial Inst., Centers for Public Health Research, Atlanta, GA USA<br />
Workshop on Varicella Surveillance: Current Status <strong>and</strong> Future Directions, Final Report <strong>and</strong> Executive Summary Final<br />
Report<br />
Black, Robert S., Battelle Memorial Inst., USA; Butler, Mary Odell, Battelle Memorial Inst., USA; Sep. 30, 1999; 56p; In English<br />
Contract(s)/Grant(s): 200-96-0599<br />
Report No.(s): PB<strong>2001</strong>-102580; NIP-99-110; Copyright; Avail: National <strong>Technical</strong> Information Service (NTIS)<br />
The purpose of this project was to prepare a summary of proceedings <strong>and</strong> recommendations of a CDC meeting convened by<br />
the National Immunization Program, Centers for Disease Control <strong>and</strong> Prevention to consider requirements for national surveillance<br />
of varicella <strong>and</strong> zoster. The meeting was held in Atlanta on March 12-13, 1998. Representatives of organizations concerned<br />
with surveillance of vaccine-preventable diseases met to: (1) discuss the current status of varicella surveillance in the US; (2) determine<br />
the epidemiologic <strong>and</strong> laboratory capacities required for effective surveillance at the state <strong>and</strong> national levels; <strong>and</strong> (3) review<br />
proposed immunization coverage <strong>and</strong> disease reduction goals for varicella.<br />
Author<br />
Conferences; Diseases; Immunology; Prevention; Surveillance<br />
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