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L 225/254 21.8.2001 Journal officiel des Communautés européennes FR 3. BIBLIOGRAPHIE (1) OECD Test Guideline Programme, Report of the Workshop on the Daphnia magna Pilot Ring Test, Sheffield University, UK, 20-21 March 1993. (2) OECD Environmental Health and Safety Publications. Series on Testing and Assessment No. 6. Report of the Final Ring Test of the Daphnia magna Reproduction Test Paris. 1997. (3) Baird D. J., Barber J., Bradley M. C., Soares A. M. V. M. and Calow P. (1991). A comparative study of genotype sensitivity to acute toxic stress using clones of Daphnia magna Strauss. Ecotoxicology and Environmental Safety, 21, pp. 257-265. (4) Elendt B. P., (1990). Selenium deficiency in Crustacea; An ultrastructural approach to antennal damage in Daphnia magna Straus. Protoplasma, 154, pp. 25-33. (5) EPA (1993). Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms. (Fourth ed.). EPA/600/4-90/027F. C. I. Weber (ed), USEPA, Cincinnati, Ohio. (6) Vigano L., (1991) Suitability of commercially available spring waters as standard medium for culturing Daphnia magna. Bull. Environ. Contam. Toxicol., 47, pp. 775-782. (7) ASTM (1988). Standard Guide for Conducting Acute Toxicity Tests with Fishes, Macroinvertebrates and Amphibians. E729-88a. American Society for Testing and Materials, Philadelphia P.A. 20 pp. (8) Baird D. J., Soares A. M. V. M., Girling A., Barber J., Bradley M. C. and Calow P. (1989). The long term maintenance of Daphnia magna Straus for use in ecotoxicological tests; problems and prospects. In: Proceedings of the 1st European Conference on Ecotoxicology. Copenhagen 1988 (H.Løkke, H. Tyle & F. Bro-Rasmussen. Eds.), pp. 144-148. (9) Parkhurst B. R., Forte J. L. and Wright G. P. (1981). Reproducibility of a life-cycle toxicity test with Daphnia magna. Bull. Environ. Contam. and Toxicol., 26, pp. 1-8. (10) Cowgill U. M. and Milazzo D. P. (1990) The sensitivity of two cladocerans to water quality variables: salinity and hardness. Arch. Hydrobiol., 120(2), pp. 185-196. (11) Korshikov (1990). Pseudokirchneriella subcapitata Hindak, F-1990. Biologice Prace, 36, 209. (12) Sims I. R., Watson S. and Holmes D. (1993). Toward a standard Daphnia juvenile production test. Environmental Toxicology and Chemistry, 12, pp. 2053-2058. (13) Sims I. (1993). Measuring the growth of phytoplankton: the relationship between total organic carbon with three commonly used parameters of algal growth. Arch. Hydrobiol., 128, pp. 459-466. (14) Dunnett C. W., (1955). A multiple comparisons procedure for comparing several treatments with a control. J. Amer. Statist. Assoc., 50, pp. 1096-1121. (15) Dunnett C. W., (1964). New tables for multiple comparisons with a control. Biometrics, 20, pp. 482-491. (16) Williams D. A. (1971). A test for differences between treatment means when several dose levels are compared with a zero dose control. Biometrics 27, pp. 103-117. (17) Williams D. A. (1972). The comparison of several dose levels with a zero dose control. Biometrics, 28, pp. 510-531. (18) Draper N. R. and Smith H. (1981). Applied Regression Analysis, second edition, Wiley, N.Y. (19) Brain P. and Cousens R. (1989). An equation to describe dose responses where there is stimulation of growth at low doses. Weed Research, 29, pp. 93-96. (20) Wilson E. O. and Bossert, W. H. (1971). A Primer of Population Biology. Sinauer Associates Inc. Publishers. (21) Poole R. W. (1974). An Introduction to quantitative Ecology. McGraw-Hill Series in Population Biology, New York, pp. 532. (22) Meyer J. S., Ingersoll C. G., McDonald L. L. and Boyce M. S. (1986). Estimating uncertainty in population growth rates: Jackknife vs bootstrap techniques. Ecology, 67, pp. 1156-1166.
21.8.2001 L 225/255 Journal officiel des Communautés européennes FR APPENDICE 1 PRÉPARATION DE MILIEUX ELENDT M7 ET M4 ENTIÈREMENT DÉFINIS Acclimatation aux milieux Elendt M7 et M4 Certains laboratoires ont éprouvé des difficultés à transférer directement les Daphnia dans les milieux M4 (1) et M7. Ils ont toutefois obtenu certains résultats en procédant à une acclimatation progressive, c'est-à-dire en transférant les animaux de leur milieu vers un milieu à 30 % d'Elendt, puis à 60 % d'Elendt et enfin à 100 % d'Elendt. La période d'acclimatation peut prendre jusqu'à un mois. PRÉPARATION Éléments en traces Différentes solutions mères (I), contenant chacune une seule substance en traces, sont tout d'abord préparées avec une eau d'une pureté adéquate, par exemple de l'eau désionisée, distillée ou obtenue par osmose inverse. À partir de ces différentes solutions mères (I), on prépare une deuxième solution mère unique (II) renfermant toutes les substances en traces (solution combinée), à savoir: Solutions mères I (substance unique) Quantité ajoutée à l'eau (mg/l) Concentration (par rapport au milieu M4) (fois) Pour préparer la solution mère combinée II, ajouter la quantité suivante de solution mère I à l'eau (ml/l) M4 M7 H 3BO 3 57 190 20 000 1,0 0,25 MnCl 2 *4H 2O 7 210 20 000 1,0 0,25 LiCl 6 120 20 000 1,0 0,25 RbCl 1 420 20 000 1,0 0,25 SrCl 2 *6H 2O 3 040 20 000 1,0 0,25 NaBr 320 20 000 1,0 0,25 Na 2MoO 4 *2H 2O 1 260 20 000 1,0 0,25 CuCl 2 *2H 2O 335 20 000 1,0 0,25 ZnCl 2 260 20 000 1,0 1,0 CoCl 2 *6H 2O 200 20 000 1,0 1,0 KI 65 20 000 1,0 1,0 Na 2SeO 3 43,8 20 000 1,0 1,0 NH 4VO 3 11,5 20 000 1,0 1,0 Na 2EDTA * 2 H 2O 5 000 2 000 – – FeSO 4 *7H 2O 1 991 2 000 – – Les solutions de Na 2EDTA et de FeSO 4 sont préparées séparément, puis mélangées et immédiatement autoclavées. Cela donne: Solution 21 Fe-EDTA 1 000 20,0 5,0
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EAU DOUCE Brachydanio rerio Danio E
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