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2 nd International Non-Wood Products Symposium 8-10 September 2011 - Isparta/TURKEY REFERENCES Anonymous, Interview with regional people in Gazipasa-Turkey (2010). AOAC. (1990). Official methods of analysis (15 th ed.). Washington DC: Association of Official Analytical Chemists. Aparicio, R., Roda, L., Albi, M.A., Gutierrez, F. 1999. Effect of various compounds on virgin olive oil stability measured by Rancimat. Journal of Agricultural and Food Chemistry, 47, 4150-4155. Baydar, H., Marquard, R., & Turgut, İ. (1999). Pure line selection for improved yield, oil content and different fatty acid composition of sesame, Sesamum indicum L. Plant Breeding, 11, 462-464. Dorman, H. J.D., Peltoketo, A., Hiltunen, R., Tikkanen M.J., 2003. Cahracterization of Antioxidant properties of de-odourized aqueous extracts from selected Lamiaceae herbs. Food Chemistry 83: 255– 62. Dumon, M.C. (1990). Recherches analytiques sur les picnogenols. These pour le Doctorat d’ Etat des Siences pharmaceutique, Universite de Bordeaux II. Fakir, H., Yaşar, S., Erbaş, S.,Özderin, S., 2011.Essential Oil Composition of Biebersteinia orphanidis Boiss. Growing in Mediterranean Region of Turkey, Asian Journal of Chemistry, Volume 23, No.8 (2011), 3767-3768. Gliszcynska-Swiglo, A., Sikorska, E. 2004. Simple reversed-phase liquid chromatography method for determination of tocopherols in edible plant oils. Journal of Chromatography A, 1048, 195-198. Göktürk Baydar, N., Akkurt, M. 2001. Oil content and oil quality properties of some grape seeds. Turkish Jounal of Agriculture and Forestry, 25,163-168. Guthrie, N., Kurowska, E.M. 2001. Anticancer and cholesterol lowering activities of tocotrienols. In: Wildman, R.E.C. (Ed), Nutraceuticals and Functional Foods, CRC Press, Boca Raton, pp. 269- 280, Florida. Hall, J.L., Flower, T.J., Roberts, R.M. 1981. Plant Cell Structure and Metabolism. Longman Inc., New York. Jayaprakasha, G.K., Selvi, T., & Sakariah, K.K. (2003). Antibacterial and antioxidant activities of grape (Vitis vinifera) seed extracts. Food Research International, 36, 117-122. Jayaprakasha, G.K., Singh, R.P., & Sakariah, K.K. (2001). Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food Chemistry, 73, 285-290. Kamal-Eldin, A., Appelqvist, L.A. 1996. The chemistry of antioxidant properties of tocopherols and tocotrienols. Lipids, 31(7), 671-701. Lampi, A.M., Kataja, L., Kamal-Eldin, A., Piironen, V. 1999. Antioxidant activities of and tocopherols in the oxidation of rapeseed oil triacylglycerols. Journal of the American Oil Chemists’ Society, 76, 749-755. Mayes, P.A. 1983. Metabolism of Lipid: In. Fatty Acids Harper's Review of Biochemistry. Medical Pub. Inc., California. P.H. Davis, R. Mill and T. Kit, Flora of Turkey and East Aegean Islands, Edinburgh University Press, Edinburgh, Vol. 2 (1966). Schuster, W.H. 1992. Ölflanzen im Europa. DLG- Verlag, Frankfurt am Main. Singleton, V.L., & Rossi, J.R. (1965). Colorimetry of total phenolics with Phosphomolybdic-phosphotungstic acid. American Journal of Enology and Viticulture, 16, 144-158. Woods, J.E., Senthilmohan, S.T., & Peskin, A.V. (2002). Antioxidant activity of prociyanidincontaining plant extracts at different pHs. Food Chemistry, 77, 155-161. 101
2 nd International Non-Wood Forest Products Symposium 8-10 September 2011 - Isparta/TURKEY In vitro Antioxidant Activity of Extract of Ankyropetalum reuteri Fenzl. Ismail Ozmen 1 ,Umut Celikoglu 2 , Hasan Ozcelik 3 , Sercan Ozbek Yazici 4* 1,2,3 Faculty of Arts and Sciences, Süleyman Demirel University, Isparta, Turkey 4 Health School, Mehmet Akif Ersoy University, Burdur, Turkey, sozbek@mehmetakif.edu.tr Abstract: Some species belonging to genera Ankyropetalum are fairly important in economy and saponin-rich plants. Saponins are glycosides found in many plant and it is well-known as a foaming agent. The materials containing saponin have been used as soap but the use of saponin is widely expanded in food industry. Saponins have also pharmacological and haemolytic properties. In the current study, ıt was investigated total phenolic content and antioxidant activity of crude extract of Ankyropetalum reuteri Fenzl. Plant was collected in June 2009 near to Adıyaman, (Turkey). The root material was dried, powdered and extracted with methanol. Antioxidant activity of extract was evaluated by using Folin Ciocalteu method, 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging activity, ferric-ion reducing antioxidant parameter (FRAP), cupric reducing antioxidant capacity (CUPRAC). Gallic acid, BHA (butylated hydroxyanisole) and trolox were used as standards.. In this study, total phenolic content of extract was found to be 4.25 µg Gallic Acid Equivalence/mg (extract). The DPPH IC 50 values of the extract and BHA were 24,3 mg/ml and 19,01µg/ml, respectively. While antioxidant capacity by using FRAP assay was found 36,29 µmol (trolox)/kg (extract), the trolox equivalent antioxidant capacity value determined by the CUPRAC assay was 49,94 µmol (trolox)/kg ( trolox). Those results are showed that the extract has low antioxidant activity of the methanolic extract compared with senthetic antioxidant. Keywords: Ankyropetalum reuteri Fenzl, Saponin, Antioxidant activity Ankyropetalum reuterı Fenzl.’den Elde Edilen Metanol Ekstraktının In Vitro Antioksidan Aktivitesi Özet: Ankyropetalum cinslerine ait bazı türler ekonomik bakımdan oldukça önemli ve saponin bakımından zengin bitkilerdir. Saponinler birçok bitkide bulunan glikozitlerdir ve köpürme ajanı olarak bilinmektedir. Saponin içeren maddeler sabun olarak kullanılmakla birlikte yiyecek endüstrisinde de yaygın olarak kullanılmaktadır. Saponinler aynı zamanda farmakolojik ve hemolitik özelliklere sahiptirler. Çalışmada Ankyropetalum reuteri Fenzl.’den elde edilen ham ekstraktın total fenolik içeriği ve antioksidan aktivitesi araştırılmıştır. Bitki Adıyaman ili civarında 2010 haziran ayında toplanmıştır ve kök kısmı kurutulup toz haline getirildikten sonra metanol ile ekstraksiyon yapılmıştır. Antioksidan aktivite, Folin Ciocalteu metodu, 1,1-diphenil-2-picril-hidrazil (DPPH) serbest radikal süpürme aktivitesi, demir indirgeme kapasitesi (FRAP), bakır indirgeme kapasitesi (CUPRAC) metodları kullanılarak değerlendirilmiştir. Gallik asit, BHA (bütillenmiş hydroksianisol) ve troloks standart antioksidan olarak kullanılmıştır. Çalışmada ekstraktın toplam fenolik içeriği 4.25 µg Gallik Asit Eşdeğeri/mg (ekstrakt) olduğu bulunmuştur. Ekstrakt ve BHA için DPPH IC 50 değerleri 24,3 mg/ml ve 19,01µg/ml’dir. FRAP yöntemi ile antioksidan kapasite 36,29 µmol (troloks)/kg (ekstrakt) olarak bulunurken CUPRAC
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