Protein Expression and Purification Series - Bio-Rad

Protein Expression and Purification Series
Flow Adaptor Description
The cam latch can be adjusted to three positions allowing the flow adaptor to be situated into a glass
column, and the O-ring to be tightened to seal the flow adaptor securely into the column so that there is no
movement once the flow adaptor has been set up (Figure 6.2).
Fitting the Flow Adaptor to the Glass Econo-Column column
Due to normal tolerances in glass diameters, each flow adaptor should be adjusted to fit to individual
columns. This will ensure optimal performance.
1. Pull the white flow adaptor body all the way up into the collar of the black flow adaptor body (See
Figure 6.3).
Figure 6.3. Preparing the flow adaptor to be fit into
a glass Econo-Column column.
2. Attach the flow adaptor body to the column by sliding the flow adaptor body onto the yellow flange on
the top of the glass column (Figure 6.4).
Figure 6.4. Attaching the flow adaptor to the glass
Econo-Column column. A) Look for the groove on
the end of the black flow adaptor body. B) Line up
the groove on the black flow adaptor body with the
top of the yellow flange on the column. C) Gently
slide the flow adaptor onto the glass column. D) The
flow adaptor should snap into place when completely
positioned on the top of the column.
Chapter 6: Handpacking a Glass Econo-Column
Column Attached to a Flow Adaptor
Figure 6.2. The three positions of the cam latch and the corresponding
effects on the O-ring. When the cam latch is in position 1, there is little
pressure on the O-ring (A), and it should slide with ease through the glass
column. When the cam latch is in position 2, the O-ring seals against
the glass column wall (B) with a small amount of movement possible for
final height adjustments. When the cam latch is in position 3, the O-ring
completely seals the flow adaptor into position (C).
A
B
C
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CHAPTER 6
HANDPACKING
COLUMNS