Protein Expression and Purification Series - Bio-Rad

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CHAPTER 2 FOCUS QUESTIONS Protein Expression and Purification Series Focus Questions for Protein Expression 1. What is a recombinant protein, and why would it be used instead of a native protein? 2. Name at least one pro and one con of using eukaryotic cells to produce a recombinant protein. When would you choose to use eukaryotic cells to produce a recombinant protein? 3. Name at least one pro and one con of using prokaryotic cells to produce a recombinant protein. When would you choose to use prokaryotic cells to produce a recombinant protein? 4. What are three considerations for recombinant protein gene design? 5. What is the log phase of a cell culture, and why is it important to recombinant protein production? 6. Why are subcultures prepared when trying to produce recombinant proteins? 7. What is the purpose of adding glucose to culture media for lac operon systems? 50 Chapter 2: Focus Questions
Protein Expression and Purification Series Focus Questions for Protein Purification Introduction 1. What is protein purification? 2. Describe lysis and why it is a necessary part of the protein purification process. Name a lysis technique. 3. Why is it important to remove DNA from the sample? 4. What enzyme is used to break down the DNA in a lysate but leaves the protein intact? 5. What is a common method of capture, or isolation, of protein? Chapter 2: Focus Questions 51 CHAPTER 2 FOCUS QUESTIONS
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CHAPTER 6 HANDPACKING COLUMNs Prote
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CHAPTER 7 BIOLOGIC LP SYSTEM PROTOC
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CHAPTER 8 BIOLOGIC DUOFLOW PROTOCOL
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CHAPTER 9 DHFR ENZYMATIC ASSAY Prot
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APPENDIX A TIPS AND HELPFUL HINTS P
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APPENDIX A TIPS AND HELPFUL HINTS P
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APPENDIX B RESULTS ANALYSIS Protein
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APPENDIX B RESULTS ANALYSIS Protein
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APPENDIX C RCF TO RPM CONVERSTION P
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APPENDIX D SETTING UP THE SMARTSPEC
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APPENDIX D SETTING UP THE SMARTSPEC
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APPENDIX E MINI-PROTEAN GEL BOX SET
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APPENDIX F BIOLOGIC LP SYSTEM SETUP
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APPENDIX F BIOLOGIC LP SYSTEM SETUP
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APPENDIX G BIOMANUFACTURING Protein
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APPENDIX G BIOMANUFACTURING Protein
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APPENDIX H FOCUS QUESTIONS INSTRUCT
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APPENDIX I GLOSSARY Protein Express
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APPENDIX I GLOSSARY Protein Express
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APPENDIX J REFERENCES, LEGAL NOTES
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Life Science Group Bulletin 1665067
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Protein Expression and Purification Series - Bio-Rad

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