Protein Expression and Purification Series - Bio-Rad

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CHAPTER 2 FOCUS QUESTIONS Protein Expression and Purification Series Focus Questions for Introduction to Chromatography 1. What is the purpose of chromatography? 2. What is the mobile phase in chromatography? 3. What is the stationary phase in chromatography? Give three examples of stationary phase. 4. Name four types of liquid chromatography techniques. 5. What is an anion and what is a cation? When would you use anion exchange chromatography versus cation exchange chromatography? 6. What is an affinity chromatography tag? Name two tags. 7. Does adding an affinity tag to the protein change the protein? Explain your answer. 52 Chapter 2: Focus Questions
Protein Expression and Purification Series Focus Questions for Centrifugation and Chromatography Instrumentation Purification 1. What is gravity chromatography? Name an advantage and disadvantage of this system. 2. Is centrifugation/spin chromatography the same as gravity chromatography? Explain your answer. 3. What are the advantages of spin chromatography? When would you choose to use centrifugation/spin chromatography and why? 4. What are the advantages of using prepacked cartridges? When would you choose to use prepacked cartridges and why? 5. What is a fraction in chromatography? Chapter 2: Focus Questions 53 CHAPTER 2 FOCUS QUESTIONS
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CHAPTER 4 11 ml CULTURE PROTOCOL Pr
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CHAPTER 5 100 ml CULTURE PROTOCOL P
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CHAPTER 6 HANDPACKING COLUMNs Prote
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CHAPTER 7 BIOLOGIC LP SYSTEM PROTOC
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CHAPTER 8 BIOLOGIC DUOFLOW PROTOCOL
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CHAPTER 9 DHFR ENZYMATIC ASSAY Prot
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APPENDIX A TIPS AND HELPFUL HINTS P
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APPENDIX A TIPS AND HELPFUL HINTS P
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APPENDIX B RESULTS ANALYSIS Protein
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APPENDIX B RESULTS ANALYSIS Protein
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APPENDIX C RCF TO RPM CONVERSTION P
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APPENDIX D SETTING UP THE SMARTSPEC
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APPENDIX D SETTING UP THE SMARTSPEC
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APPENDIX E MINI-PROTEAN GEL BOX SET
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APPENDIX F BIOLOGIC LP SYSTEM SETUP
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APPENDIX F BIOLOGIC LP SYSTEM SETUP
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APPENDIX G BIOMANUFACTURING Protein
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APPENDIX G BIOMANUFACTURING Protein
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APPENDIX H FOCUS QUESTIONS INSTRUCT
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APPENDIX I GLOSSARY Protein Express
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APPENDIX I GLOSSARY Protein Express
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APPENDIX J REFERENCES, LEGAL NOTES
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Life Science Group Bulletin 1665067
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Protein Expression and Purification Series - Bio-Rad

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