Protein Expression and Purification Series - Bio-Rad

Protein Expression and Purification Series
5.
6.
Appendix E: Gel Box Setup
The arms of the clamping frame push the short plates of each gel cassette up against the notch
in the green gasket, creating a leak-proof seal (check again to make certain that the short plates
sit just below the notch at the top of the green gasket). At this point, the sample wells can be
washed-out with running buffer (Figure 2D).
Note: If running more than two gels, repeat steps 1A–D with the Companion Running Module.
Important Note: Do not attempt to lock the green arms of the clamping frame, without first
ensuring that the gel cassettes are perfectly aligned and stabilized against the notches on the
green gaskets of the module. To prevent the gels from shifting during the locking step, firmly and
evenly grip them in place against the core of the module with one hand.
Run the gel(s): Prepare, load and electrophorese the samples according to the instructions
in Chapters 4 or 5, depending on whether you are performing the centrifugation process or
chromatography instrumentation process for purification. At the completion of the run, disconnect
the cell and remove the cassette.
CAUTION: When running one or two gels only, DO NOT place the Companion Running
Module in the tank. Doing so will cause excessive heat generation and prevent
electrophoretic separation.
Removing the Gel from the Cassette after the Gel has Been Run
1. Open the cassette:
Align the arrow on the opening lever with the arrows marked on the
cassette (see Figure 3). Insert the lever between the cassette plates at all four locations and apply
downward pressure to break each seal. Do not twist the lever. Gently pull apart the two plates
beginning from the top of the cassette.
2. Remove Gel:
Gently remove the gel from the cassette.
Figure 3. Opening the gel cassette.
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APPENDIX E
MINI-PROTEAN GEL
BOX SET UP