Protein Expression and Purification Series - Bio-Rad

Protein Expression and Purification Series
Figure 8.20. Selecting the fraction collector in the device set up window.
2.
3.
4.
Click on BioFrac and click the OK button. You will now see BioFrac fraction collector in the Devices in
Setup box. The F1 Rack (12–13 mm tubes) is automatically selected. Click OK to choose.
In the Gradient Pump section of the setup screen enter your buffer names. In the Buffer A field, type in
2x PBS, pH 7.2. In the Buffer B field, type in 2x PBS + 250 mM Imidazole, pH 8.0.
You are now ready to program the separation steps for your method. To program your method, press
the Protocol icon on the tool bar.
3. Program the Method Protocol
From the Options pull-down menu, ensure that Use Volume (ml) is selected so that the programming base
is Volume (Figure 8.21).
Figure 8.21. BioLogic DuoFlow system setting base to Volume.
Programming the fraction collector (BioFrac fraction collector)
1.
2.
3.
4.
From the left side of the screen, press the fraction collection icon (Figure 8.22).
In the pop-up window that appears, choose Collection Windows.
Make sure the correct rack type is displayed (F1 12-13 mm tubes).
Click on the tab Collection/Windows.
Chapter 8: Purification Protocol for BioLogic DuoFlow System
179
CHAPTER 8
BIOLOGIC DUOFLOW
PROTOCOL