Protein Expression and Purification Series - Bio-Rad

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Protein Expression and Purification Series DHFR Enzymatic Assay • Ensure the DHF is reconstituted in 10x PBS. • Ensure the NADPH is reconstituted in 1x PBS. • Ensure the DHF and NADPH are used within three to four hours. The reconstituted DHF and NADPH cannot be frozen to retain or extend the activity and shelf life. • The enzymatic reaction occurs very quickly so make sure to add the DHF to the NADPH solution and then place it immediately in the spectrophotometer. It is best to do this step next to the spectrophotometer to ensure no data points are missed. • Make sure the spectrophotometer is turned on at least 30 minutes ahead of time to allow the lamp to warm up. • If using trUView cuvettes, make sure the frosted side is facing the beam path. The frosted side of the trUView cuvettes contains a tiny clear window through which the UV reading is taken. 24 Timeline for the Laboratory Course: Centrifugation Purification
Protein Expression and Purification Series Chromatography Instrumentation Purification Workflow The timeline will depend greatly on the level of the students, the length of class periods, and whether other techniques and analyses are performed in addition to the basic protocol. Timeline for the Laboratory Course: Chromatography Instrumentation Purification 25
Page 1: Biotechnology Explorer TM Protein E
Page 4 and 5: Protein Expression and Purification
Page 34 and 35: CHAPTER 1 BACKGROUND Protein Expres
Page 50 and 51: CHAPTER 2 FOCUS QUESTIONS Protein E
Page 56 and 57: CHAPTER 3A ADVANCE PREP CENTRIFUGAT
Page 72 and 73: CHAPTER 3B ADVANCE PREP INSTRUMENTA
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CHAPTER 3B ADVANCE PREP INSTRUMENTA
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CHAPTER 4 11 ml CULTURE PROTOCOL Pr
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CHAPTER 5 100 ml CULTURE PROTOCOL P
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CHAPTER 6 HANDPACKING COLUMNs Prote
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CHAPTER 7 BIOLOGIC LP SYSTEM PROTOC
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CHAPTER 8 BIOLOGIC DUOFLOW PROTOCOL
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CHAPTER 9 DHFR ENZYMATIC ASSAY Prot
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APPENDIX A TIPS AND HELPFUL HINTS P
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APPENDIX A TIPS AND HELPFUL HINTS P
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APPENDIX B RESULTS ANALYSIS Protein
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APPENDIX B RESULTS ANALYSIS Protein
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APPENDIX C RCF TO RPM CONVERSTION P
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APPENDIX D SETTING UP THE SMARTSPEC
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APPENDIX D SETTING UP THE SMARTSPEC
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APPENDIX E MINI-PROTEAN GEL BOX SET
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APPENDIX F BIOLOGIC LP SYSTEM SETUP
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APPENDIX F BIOLOGIC LP SYSTEM SETUP
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APPENDIX G BIOMANUFACTURING Protein
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APPENDIX G BIOMANUFACTURING Protein
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APPENDIX H FOCUS QUESTIONS INSTRUCT
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APPENDIX I GLOSSARY Protein Express
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APPENDIX I GLOSSARY Protein Express
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APPENDIX J REFERENCES, LEGAL NOTES
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Life Science Group Bulletin 1665067
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Protein Expression and Purification Series - Bio-Rad

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