Protein Expression and Purification Series - Bio-Rad

CHAPTER 4
11 ml CULTURE
PROTOCOL
Protein Expression and Purification Series
Figure 4.1. Streaking a plate to generate single colonies.
5.
Place the plate upside down inside the incubator overnight at 37°C. Once colonies have grown to
1 mm in diameter, they may be stored in a sealed bag upside down at 4ºC until ready to use for up to
one month.
6. E. coli forms off-white colonies that are uniformly circular with smooth edges. Avoid using plates with
contaminant colonies.
92 Chapter 4: 11 ml Culture Protocol
for Centrifugation Purification