Protein Expression and Purification Series - Bio-Rad

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CHAPTER 2 FOCUS QUESTIONS Protein Expression and Purification Series Focus Questions for SDS-PAGE Electrophoresis 1. What are four ways to quantify the amount of protein in a sample? 2. What does primary (1°), secondary (2°), tertiary (3°), and quaternary (4°) protein structure refer to? 3. Describe SDS-PAGE analysis. Why would you use SDS-PAGE to analyze your samples? 4. What are BME and DTT and what do they do to a protein? 5. Describe the components and function of each component in Laemmli sample buffer. 54 Chapter 2: Focus Questions
Protein Expression and Purification Series Focus Questions for DHFR Enzyme Activity 1. Which cofactor is required in the conversion of dihydrofolate to tetrahydrofolate? 2. What is the purpose of the cofactor? 3. Define oxidation. Define reduction. 4. In the conversion of DHF to THF, which compound donates the hydride ion to DHF? 5. What information does the DHFR enzyme activity provide in the context of recombinant protein expression and purification? Chapter 2: Focus Questions 55 CHAPTER 2 FOCUS QUESTIONS
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Biotechnology Explorer TM Protein E
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CHAPTER 6 HANDPACKING COLUMNs Prote
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CHAPTER 7 BIOLOGIC LP SYSTEM PROTOC
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CHAPTER 8 BIOLOGIC DUOFLOW PROTOCOL
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CHAPTER 9 DHFR ENZYMATIC ASSAY Prot
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APPENDIX A TIPS AND HELPFUL HINTS P
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APPENDIX A TIPS AND HELPFUL HINTS P
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APPENDIX B RESULTS ANALYSIS Protein
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APPENDIX B RESULTS ANALYSIS Protein
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APPENDIX C RCF TO RPM CONVERSTION P
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APPENDIX D SETTING UP THE SMARTSPEC
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APPENDIX D SETTING UP THE SMARTSPEC
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APPENDIX E MINI-PROTEAN GEL BOX SET
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APPENDIX F BIOLOGIC LP SYSTEM SETUP
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APPENDIX F BIOLOGIC LP SYSTEM SETUP
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APPENDIX G BIOMANUFACTURING Protein
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APPENDIX G BIOMANUFACTURING Protein
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APPENDIX H FOCUS QUESTIONS INSTRUCT
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APPENDIX I GLOSSARY Protein Express
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APPENDIX I GLOSSARY Protein Express
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APPENDIX J REFERENCES, LEGAL NOTES
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Life Science Group Bulletin 1665067
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Protein Expression and Purification Series - Bio-Rad

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