Protein Expression and Purification Series - Bio-Rad

CHAPTER 8
BIOLOGIC DUOFLOW
PROTOCOL
Protein Expression and Purification Series
Figure 8.18. BioLogic DuoFlow system New Method dialog box.
Figure 8.19. Setup editor. In the Setup screen you will select the instruments and devices to be used for the GST-DHFR-His
purification method. The icons grouped on the left side of the screen show all the instruments and devices that can be connected
to the BioLogic DuoFlow system. The list of devices in the right box (Devices in Setup) identifies those devices selected for use with
a specific method. The initial default Devices in Setup are a UV detector, conductivity monitor, and an AVR7-3 inject valve. These
come standard with the BioLogic DuoFlow system.
2. Program the Instrument Setup
1.
Click on the Fraction Collector button in the Available Devices box (Figure 8.20). A dialog box will
appear asking you to choose the type of collector (e.g., a generic collector, a Model 2110, or a
BioFrac fraction collector).
Note: The following instructions pertain to programming the BioFrac fraction collector. If you have a
different fraction collector, please consult that instrument’s instruction manual.
178 Chapter 8: Purification Protocol for BioLogic DuoFlow System