Protein Expression and Purification Series - Bio-Rad

Protein Expression and Purification Series
Appendix E : Setting up a Mini-PROTEAN Tetra Gel Box
using Mini-PROTEAN TGX Gels
Mini-PROTEAN TGX Gel Set Up
1. Remove comb:
Position both thumbs on the ridges of the comb. Remove the comb by pushing
upward in one smooth continuous motion (Figure 1).
2. Remove tape:
Pull the green tape gently to remove from the bottom of the cassette
(Figure 1).
3. Rinse wells:
Use a syringe wash bottle or a disposable transfer pipette to rinse the wells with running
buffer. Straighten the sides of the wells, if necessary.
Figure 1. Mini-PROTEAN TGX gel.
Assemble the Mini-PROTEAN Tetra Cell
Note: When running two gels only, use the Electrode Assembly (the one with the banana plugs), NOT
the Companion Running Module (the one without the banana plugs). When running four gels, both the
Electrode Assembly and the Companion Running Module must be used, for a total of four gels (two gels
per assembly).
1. Set the clamping frame to the open position on a clean flat surface (see Figure 2A).
2.
Place the first gel sandwich or gel cassette (with the short plate facing inward) onto the gel
supports. Gel supports are molded into the bottom of the clamping frame assembly; there are two
supports in each side of the assembly. Note that the gel will now rest at a 30° angle, tilting away from
the center of the clamping frame.
Appendix E: Gel Box Setup
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APPENDIX E
MINI-PROTEAN GEL
BOX SET UP