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Protein Expression and Purification Series - Bio-Rad

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<strong>Protein</strong> <strong>Expression</strong> <strong>and</strong> <strong>Purification</strong> <strong>Series</strong><br />

Focus Questions for <strong>Protein</strong> <strong>Purification</strong> Introduction<br />

1. What is protein purification?<br />

2. Describe lysis <strong>and</strong> why it is a necessary part of the protein purification process. Name a lysis<br />

technique.<br />

3. Why is it important to remove DNA from the sample?<br />

4. What enzyme is used to break down the DNA in a lysate but leaves the protein intact?<br />

5. What is a common method of capture, or isolation, of protein?<br />

Chapter 2: Focus Questions<br />

51<br />

CHAPTER 2<br />

FOCUS QUESTIONS

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