Book of Abstracts - Geyseco

P - Posters
3, 400 new propagules seeded), forest under high tidal influence
(trial 4, 400 new propagules seeded), and an area where the original
mangrove forest had been clear-cut (trial 5, 400 new propagules
seeded). The survival and height of the new plants were
evaluated once every two month.
The better results were shown in trials 5 and 3, were 100% of
survival was reached, followed by trial 1. The maximum growth
was measured in trial 3 (62.36 cm as a mean), trial 1 (47.7), and
5 (41.4). The main causes of seedling death were natural events
(high tidal amplitude and current, crab herbivory) and antropic
factors (sticks collection). The natural regeneration (trial 2) was
scarce, these results highlighting the importance of reforestation,
together with reduced antropic activity, to preserve the mangrove
ecosystem.
P09-009: GENESIS OF GLANDULAR TRICHOMES AND
BIOSYNTHESIS OF ESSENTIAL OILS IN LEAVES OF
MINT: EFFECT OF TEMPERATURE AND LIGHT QUA-
LITY
Kirichenko E 1 * - Kurilov, D 2 - Olekhnovich, L. 1 - Tarakanov ,
I. 3 - Bidukova, G. 1 - Babosha, A. 1 - Enina, O. 1 - Smirnova I. 1 -
Luu Dam, C. 4
1
“N.V. Tsitsin” Main Botanical Garden of RAS
2
“N.D. Zelinsky” Institut of Organic Chemistry of RAS
3
K.A Timiryazev Agricultural Academy
4
Vietnam National Museum of Nature
*Corresponding author, e-mail: evkir@list.ru
The objective of this work was to elucidate the relationship
between formation of glandular trichomes and biosynthesis of
essential oils in plants related to genus Mentha L. Experiments
were performed with varieties and wild forms, representing
M.canadensis, M.longifolia, M. piperita and M. arvensis. Development
оf glandular trichomes was studied using scanning
electron microscopy. Component composition of essential oils
was analised by method of chromato-mass-spectrometry (Agilent
Thechnologies). Identification of components was carred out
using the library of mass-spectra NIST 05 and Kovach indices.
We showed that secretory structures accumulating essential oils
were genus-specific. The glandular apparatus was formed mainly
during initiation of leaf primordia. In the growing leaf, the glandular
trichomes were distributed irregularly along the surface of
low and upper epidermis. We concluded that plant potential to
produce essential oils was determined by the number of trichomes
in a leaf, leaf number, duration of leaf ontogenesis.Temperature
and light quality were shown to affect the genesis of glandular
trichomes at the stage of initiation of leaf primordia. These
factors changed the ratio between different types of secretory
structures, their development and aging. Mature trichomes often
collapsed and essential oils were volatilized. In total,we detected
78 individual components. This allowed us to determined the
gradient of mentol content in the leaves of different storeis and
the dynamics of major components in the stems of different years
of vegetation as dependent on formation of glandular trichomes.
Significance of longterm predetermination of morphogenetic
phenomena in the life cycle of mint plant was appreciated.
P09-010: SECONDARY SUCCESSION OF THE ORIGI-
NAL FLOODPLAIN FORESTS IN THE VÁH RIVER
AREA (SLOVAKIA) RESULTED FROM HYDROLOGI-
CAL REGIME CHANGES
Ábrahámová, A.* - Kollár, J. - Šimonovic, V.
Institute of Landscape Ecology, Slovak Academy of Sciences
*Corresponding author, e-mail: aniko.abrahamova@savba.sk
In Slovakia, Váh river presents the most intensively utilized river.
First of all, there was constructed a set of dams and hydropower
plants resulting in essential changes in hydrological regime.
In the article, we focus on its part between the cities of
Sereď and Nové Mesto nad Váhom (western Slovakia). Decrease
of underground water and flood frequency caused drying out of
original floodplain forest vegetation (stands of Salici-Populetum
association made of mainly hygrophilous species) and its successional
change into the more xerophilous types, especially in the
belt contacting the river (agradated area) made of gravel or sandy
sediments. Such degrataged stage is provisionally named as Crataegus
monogyna-Populus nigra community. Its floristic composition
is typical by mixture of ecologically different species. The
tree layer is usually poorly covered and made of Populus nigra,
while shrub layer is often well-covered and made of Prunus spinosa,
Crataegus monogyna etc. The herb layer is formed by combination
of mainly meadow species (Galium mollugo agg., Poa
angustifolia, Arrhenatherum elatius, Tithymalus cyparissias), ruderal
species (Echinops sphaerocephalus, Elytrigia repens, Convolvulus
arvense) and partly also species typical for floodplain
forests (Rubus caesius, Urtica dioica, Galium aparine).
Key words: floodplain forest, secondary succession, Crataegus
monogyna-Populus nigra community, Váh river, hydrological
regime
P09-011: POSSIBLE CONTRIBUTION OF MITOTIC
GENE CONVERSION IN THE CONSERVATION OF MA-
TERNAL HAPLOTYPE OF PEROXIDASE GENES
Kupriyanova, E.* - Ezhova, T.A.
Department of Genetics, The M.V. Lomonosov Moscow State
University
*Corresponding author, e-mail: ekupriyanova@gmail.com
The distribution of variation in a genome is the result of an intricate
interplay between mutation, recombination, selection, and
demography and is influenced by the reproductive system and
ecological constraints. Important observations have emerged
from the analyses of several Arabidopsis loci that have been subjected
to comparative sequencing in this cruciferous weed: (i) a
number of genes have alleles that fall into two distinct classes of
haplotypes, and (ii) there is more recombination than might be
expected, given that Arabidopsis is a selfer. Molecular-genetic
mapping of the area of localization of tandemly duplicated anionic
peroxidase genes AtPrx53 and AtPrx54 was performed. It was
stated that this area is the recombination hotspot where recombination
frequency was 480 times higher than the V chromosome
average. The cloning and DNA sequencing of recombinant chromosomes
has shown that the alleles of peroxidase genes posess
mosaic structure. This indicates that abnormal high frequency
of recombination was the result of conversion mechanisms. The
analysis of codominant DNA marker`s segregation demonstrated
that conversion events lead to the elimination of heterozygosity
in hybrid plants; a proportion of plants homozygous for the maternal
alleles of AtPrx53 and AtPrx54 was increased. Thus, unlike
other recombination hotspot, which generates polymorphism
by creating new recombinant alleles, we identified conversion
hotspot, which eliminate heterozygosity and restore the maternal
haplotype. It can be suggest that mitotic gene conversion can be
used by plants to preserve of maternal haplotypes which possess
higher adaptive value in specific environmental conditions.
The study was supported by grants from RFBR program (09-04-
01639).
P09-012: FLAVONOIDS IN SOME EUPHORBIA SPECIES
OF SUBSECTION ESULAE
Kaveh, M. 1 - Noori, M. 2
1
Department of Biology, Azad University-Borujerd Unit-Ira,
e-mailm: e.kaveh@gmail.com
2
DepartmeonftBiology, Faculty of Science, University of Arak,
Arak-Iran, e-mail:m-noori@araku.ac.ir
Flavonoids occur widely in plants and are a biologically major
and chemically diverse group of secondary metabolites. They are
also beneficial for the plant itself as physiological active compounds,
as stress protecting agents, as attractants or as feeding
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