Book of Abstracts - Geyseco

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P - Posters Aprile, G. G. - Migliozzi, A. - Catalano, I. - Di Salvatore, M. - Mingo, A - DIARBOPAVE - Università degli Studi di Napoli Federico II Lichens are among the most reliable biological indicators to assess direction and intensity of environmental changes. Vesuvius volcano was interest during last century by contrasting effects of both natural and anthropic disturbance. Volcanic eruption of 1943-44 opened a new fresh primary succession on lavic soils, but the impact of human activities lead to a heavy degradation of natural ecosystems. Since 1995, the volcano area is protected as a national reserve. With the aim of tracking the impact of such processes on biodiversity and environmental quality, Vesuvius lichen distribution was monitored over space and time. Past records by herbarium collections of nineteenth century were censed and reported to present criteria of classification. These data were entered in a GIS database and compared with two recent datasets, one preceding (1980) and the other following (last decade) the starting of protection regime over this territory. Multivariate techniques of analysis were employed to detect the different directions of change and to relate them to both natural and anthropic variables. About 270 taxa were recorded, most of which bark or rock crustose lichens from acid to sub-neutral substrata. A relative stability of poleophoby was observed, though indicators of eutrophication tended to increase in recent times. Other indicators, mainly related to aridity, successional stage and phytoclimatic range, are discussed in this work in relation to the changing environmental conditions of Vesuvius volcano. P14-017: IMPACT CLIMATE CHANGE ON YIELD AND BIOCHEMICAL COMPOSITION OF WHEAT SEEDS Abdumanon, A. - Kasimova, G.F. - Ergashev, A. - Karimov, Kh. H. - Saboiev, I.A. - Abdullaev, S. F. - Jumaev, K. - Rahimov, M. Institute of plants physiology and genetics of Academy sciences Republic of Tajikistan This paper presents the results of study effect of different climatic years on the parameters of the biological and economic productivity of plants and biochemical quality grain promising sorts of wheat. The analysis shows that January and November 2006 year, February and March 2007 year and February 1 - March 2009 year were periods with high monthly rainfall in the range. The results show that if in the period to September 2007 to August 2008 year annual precipitation was 358.7 mm, this value in the period September 2008-August 2009 year amounted to 905.6 mm, which is 2.53 times higher. During all these periods the monthly precipitation totals exceeded the norm from 1,3 to 1,73 times. Concerning sorts Kauz and Sham grown under normal climatic conditions, it may be noted that while the seed weight of wheat grown in 2008 year, slightly higher compared to the year 2009 year, but the protein content is lowered. The number of grains per ear and less in 2009, however, the number of seeds from year to year differences is minor. However, the mass of 1000 grains in 2009 for these plants was more favorable than in 2008 year. Comparative evaluation of biochemical parameters for sort Sham showed that the protein content almost does not change depending on climatic conditions in 2008 and 2009 year, despite significant differences in the sizes of seeds. The report discusses the impact of different climatic years to electrophoreticaly separation and identification of proteins in polyacrylamide gel. This work was supported by the International Science and Technology Center (Project T-1635) P14-018: THREE YEARS OF SEASONAL VARIATIONS IN ECOPHYSIOLOGICAL CHARACTERISTICS AND UV PROTECTION MECHANISMS IN TWO MOSSES FROM A SUBALPINE STREAM Tomás, R. - Fabón, G. - Monforte López, L. - Martínez-Abaigar, J. Núñez-Olivera, E. University of La Rioja We studied the relationships between environmental variables and the physiology of two aquatic mosses (Bryum pseudotriquetrum and Fontinalis antipyretica) in a subalpine unforested stream over a three year period. Neither environmental or physiological variables showed significant interannual variations. Most environmental variables (water temperature, stratospheric ozone, and photosynthetic, UV-A and UV-B radiation) showed distinct seasonal variations, but only a few physiological ones did. In both species, photoprotection variables (the activity of the xanthophyll cycle and the bulk UV absorbance of the methanolextractable UV-absorbing compounds, MEUVAC) varied more seasonally than variables related to physiological activity, such as the sclerophylly index and chlorophyll fluorescence parameters (Fv/Fm and FPSII). Changes in physiological activity would be attenuated by the buffering capacity of water with respect to the influence of environmental factors, and dynamic variables like Fv/Fm and FPSII would be little determined by cyclic environmental factors. In B. pseudotriquetrum, both MEUVAC and kaempferol 3,7-O-diglycoside (a potentially UV-protective flavonoid) were positively associated with radiation levels, whereas in F. antipyretica photoprotection mechanisms were not correlated with any environmental variable. In addition, MEUVAC was 3–4 fold higher in B. pseudotriquetrum than in F. antipyretica. Thus, different photoprotection mechanisms, with a different environmental regulation, can be suggested for these two species. DNA damage was not found in any sample, probably because both species displayed efficient DNA repair mechanisms. P14-019: INTERACTIVE EFFECTS OF OZONE AND CO2 ON GRWOTH, TIELD AND PHYSIOLOGY OF POTATO Hassan, I. - Hassan, I.A. Alexandria University Potato (Solanum tuberosum L. cv. Kara) was grown in open-top chambers (OTCs) in Northern Egypt at ambient (ca 350 ppm) or doubled CO2 (ca 690 ppm) either in charcoal-filtered air (15 nl l-1) or in non-filtered ambient air (78 nll-1 O3) to investigate the changes in physiology and yield under long-term elevated CO2 and/or O3 throughout 100 days. Ambient O3 level reduced net photosynthetic rates, number and weight of tubers by 18, 41 and 21%, respectively, whereas elevated CO2 caused the opposite effect where it increased the same parameters by 44, 37 and 20%, respectively. Significant O3 x CO2 interactions were detected. However, O3 caused an increase in GR and POD by 18 and 35%, respectively, while CO2 caused an increase in POD only by 46%, and there was no effect of O3 and/or CO2 on other enzymes. The results of this study are discussed in relation to predicted atmospheric changes. Key words: Open-top chambers (OTCs) - Potato (Solanum tuberosum) – O3 – CO2 – photosynthesis – Stomatal Conductance – antioxidant enzymes – growth and yield. Superoxide dismutase (SOD); Glutathione Reductase (GR); Ascorbate peroxidase (APX); Guaiacol peroxidase (POD) P14-020: INFLUENCE OF MICROCLIMATE ON GRAPE- BERRY PROTEOME Bordey, Y. 1 - Kappel, C. 1 - Magnin, N. 1 - Lapaillerie, D. 2 - Dupuy, J. W. 2 - Vilain, S. 2 - Bonneu, M. 2 - Gomes, E. 1 - Delrot, S. 1 - Trossat Magnin, C. 1 1 ISVV-UMR EGFV 1287-INRA, Bordeaux, France 2 Plate-forme Protéomique, Université Bordeaux 2, Bordeaux, F As many cultivated crops, grapevine may be particularly susceptible to climate change and hence, berry quality and production might be highly changed (White et al. 2006). Modification of the phenology timing and metabolite composition has been already observed (Pereira et al. 2006). In order to improve the knowledge on the impact of the microclimate on grape berries, we studied P
FESPB 2010 - XVII Congress of the Federation of European Societies of Plant Biology changes in the proteome of field grown sun-exposed or shaded berries on the east or west side of the row and inside or outside the canopy. The proteome analysis from whole berries by 2-D PAGE revealed around 900 total proteins. Principal component analysis done with all protein spots ascertained three statistically different proteomes. This result suggested that microclimate conditions are significantly distinct according to berry position within the grape. Among proteins differentially expressed, some (rubisco, chalcone isomerase, abscissic stress ripening, 14-3-3 isoforms, thioredoxin-dependent peroxidise, ATP synthase and carotenoid dioxygenase) presented high variation of expression levels. The expression analysis by qPCR of several flavonoid gene showed that their transcript amounts were modified by berry microclimate. These results give particularly new insights in berry proteome and show microclimate-induced changes. Also, these data raise questions about the role in berries of proteins which abundance is modulated by microclimate changes. photodegradation on mass loss and subsequent biotic decomposition. We harvested litter from 25 native and introduced species growing in temperate ecosystems in Argentina. While direct photodegradative losses were relatively small overall, the magnitude of the effect with attenuation of UV and visible light was 12% and 65% respectively. When these litter samples were then placed on the soil surface for biotic incubation, we observed a strong and consistent (P
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Page 206 and 207: Author Index Aarts, M.G. S18-002 Ab
Page 208 and 209: Cattivelli, L. P01-031 Cawly, J. P1
Page 210 and 211: Gallego, B. P17-037 Gallego, P.P. P
Page 212 and 213: Kersten, B. P10-017 Keskin, O. P05-
Page 214 and 215: Miguel, C. S02-001, P01-122 Milhinh
Page 216 and 217: Ramiro, M. P09-018 Ramon, M P13-002
Page 218 and 219: Tanimoto, E. P01-045 Tarakanov, I.
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