Book of Abstracts - Geyseco
Book of Abstracts - Geyseco
Book of Abstracts - Geyseco
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03 - S - Selected <strong>Abstracts</strong> for Oral Presentations<br />
in propane cooled with liquid N2 the leaf pieces were<br />
sectioned with a cryo-microtome (20 μm) and freeze dried. Two<br />
regions <strong>of</strong> tealeaf cross-section (epidermis–mesophyll, xylem–<br />
phloem; 80 x 80 μm 2 ) were raster scanned with 1.7 keV excitation<br />
energy to reach the Al-K absorption edge, and then again<br />
with 2.2 keV excitation energy to reach the P-K absorption edge.<br />
Al was localized mainly in the cell walls <strong>of</strong> the leaf epidermal<br />
cells where Al was not associated with P or Si. Mesophyll cell<br />
walls emitted a weak Al signal, while no Al signal was obtained<br />
from the symplast. Contrastingly, Al was more-or-less evenly<br />
distributed in the xylem–phloem region, with a slightly higher<br />
signal obtained from the phloem region. The Al signal was significantly<br />
lower than that recorded from the epidermis–mesophyll<br />
region. However, the localization <strong>of</strong> Al in the phloem region suggests<br />
that tea plants are able to retrieve Al from the transpiration<br />
stream and to redistribute it via phloem transport. LEXRF spectro-microscopy<br />
pro<strong>of</strong>ed to be a reliable tool for Al localization<br />
in plant tissues.<br />
Acknowledgements: Supported by BFU2007-60332/BFI and EU<br />
TWIN MIC 20095369<br />
S
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