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SELECTED ABSTRACTS FOR PRESENTA- ORAL TRACTS TIONS ORAL SENTA- S S01-001: REGULATION OF THE MECHANO-SENSITIVE GENE PTAZFP2 Gourcilleau, D.* - Martin, L. - Leblanc-Fournier, N. - Julien, J.L. Université Blaise Pascal *Corresponding author e-mail: delphine.gourcilleau@univbpclermont.fr In their natural environment, plants are continuously exposed to wind loads, characterized by a high variability of frequencies and intensities. In response to mechanical loadings, plants exhibit generally a decrease in longitudinal growth, an increase in diameter growth and in rooting. This syndrome of growth responses has been called thigmomorphogenesis. In order to grow in continuously changing windy conditions, plants may have to develop acclimation processes. However, molecular mechanisms involved in plant acclimation to recurring and successive mechanical loadings are not well characterized. Recently, through the analysis of the short-time effects of quantified stem bending on young poplars, we demonstrated the rapid induction of PtaZFP2 expression, a gene encoding a putative Cys2/His2 zinc finger transcription factor. The PtaZFP2 transcripts accumulate 10 min after a single bending and only in strained tissues. The relative abundance of PtaZFP2 transcripts was linearly correlated with the amount of applied mechanical solicitation. By comparing the effect of successive bending on this early mechano-sensitive gene, our results indicate that PtaZFP2 mRNA accumulate to a lesser extent after two bendings than after a single one. These results clearly show a partial desensitization of plants to recurrent successive bendings. Our objectives are now to identify molecular actors upstream PtaZFP2 in order to understand its regulation in the mechanosensing pathway. Furthermore, to characterize the kinetics of accommodation processes and the tissues involved in mechanosensing, the effect of single and repeated bending are studied, at the protein level, on PtaZFP2 accumulation and localization. S01-002: ARABIDOPSIS RESPONSE TO HIGH TEMPE- RATURE IS MEDIATED BY LIGHT INTENSITY François, V.* - Pantin, F. - Dauzat, M. - Rolland, G. - Bédié, A. - Muller, B. - Vile, D. INRA-SupAgro – UMR 759 Laboratoire d’Ecophysiologie des Plantes sous Stress Environnementaux (LEPSE), Montpellier, France *Corresponding author e-mail: fvasseur95@hotmail.fr Environmental constraints, such as temperature, water and light, often act simultaneously in the field limiting plant growth and development. Although the effects of separate stresses on plants have been described at the molecular level, little is known about the mechanisms involved in their integrated response to interacting environmental factors, particularly to long-term exposure to moderate stresses. Moreover, only a few studies have shown relationships between gene expression, physiological processes and growth in different environmental conditions. Arabidopsis thaliana ecotypes and mutants were cultivated in 03 - S - Selected Abstracts for Oral Presentations controlled conditions to study the influence of light and water availability on plant responses at high temperature. Precisely, we investigated the links between rosette growth, leaf morphology, photosynthesis, and molecular markers of carbon status. Furthermore, the analysis of particular biological functions was complemented using specific mutants. Plants showed a multilevel response to high temperature. Growth was reduced by high temperature while specific leaf area increased. Furthermore, drastic hyponastic movements of leaves occurred, suggesting that responses could be modulated by light intensity. While low light (70 μmol m -2 s -1 ) amplified the deleterious effects of high temperature, high light (330 μmol m -2 s -1 ) restored the growth achieved at control temperature and abolished hyponastic movements. Taken together, results showed that responses to high temperature depend on light availability. Physiological and gene expression data, together with interacting effects of water deficit, confirm the major role of energy balance in plant response to high temperature. S01-003: AQUATIC ADVENTITIOUS ROOTS OF THE WETLAND PLANT HALORAGIS BROWNII CAN PHO- TOSYNTHESIZE: IMPLICATIONS FOR FLOODING TO- LERANCE Rich, S.* - Ludwig, M. - Pedersen, O. - Colmer, T. The University of Western Australia *Corresponding author e-mail: sarah.rich@grs.uwa.edu Whan flooded, many plants produce adventitious aquatic roots from submerged stems. These roots supplement, or replace, the primary root system that often dies during soil flooding. Adventitious aquatic roots can form chloroplasts and,therefore, potentially contribute to plant survivial during flooding through photosynthate and O 2 production. The wetland plant Haloragis brownii (Hook.f.) Schindl.) grows an extensive stem-borne aquatic roots system (up to 29 % of total plant dry mass) during flooding. Chlorophyll is detected in these roots within days of emergence, even when plants are grown at low light levels. The aquatic roots have a complete photosynthetic pathway, producing O 2 and fixing carbon at rates similar to submerged stems, but four-fold lower than leaves. Microelectrode and 14 CO 2 -uptake experiments showed that these roots are able to produce significant amounts of carbon and O 2 , lowering inputs needed from the shoot for growth and development. The contribution of H. brownii aquatic adventitious roots to their own metabolic needs is beneficial to whole plant survival during flooding as it mediates the negative effects of energetic stress and explains the capacity for prolific growth of these new roots, which accompanies a flooding event. S01-004: NEW INSIGHTS INTO AN OLD STORY: ZMASR1 EXPRESSIONENHANCED DROUGHT STRESS TOLERANCE IN MAIZE Virlouvet, L.¹* - Jacquemot, M.P.¹ - Corti, H.¹ - Gilard, F.² - Bouton, S.¹ - Remoue, C.² - Valot, B.¹ - Riviere, N.³ - Tcherkez, G. 4 - Trouverie, J.¹ - Damerval, C.² - Gerentes, D.³ - Falque, M.¹ - Rogowsky, P.¹ - Noctor, G. 4 - Zivy, M.² - Coursol, S.¹ ¹ Institut national de la recherche agronomique (INRA), France ²Centre National de la Recherche Scientifique (CNRS), France ³Biogemma Auvergne, Ferrand Cedex, France 4 Univ Paris-Sud, Institut de Biotechnologie des Plantes, Orsay, France *Corresponding author: rennesson@moulon.inra.fr Maize is particularly sensitive to drought stress at reproductive stages with a strong impairment of gynoecium development. As such, identification of factors that confer drought stress tolerance would pave the way for increasing agricultural productivity. Here, we assessed the role of the candidate gene Zea mays abscisic acid-, stress- and ripening-induced 1 (ZmASR1) for drought stress tolerance in maize. Quantitative RT-PCR studies with S
FESPB 2010 - XVII Congress of the Federation of European Societies of Plant Biology gene-specific primers for six of the nine ZmASRs identified in the maize genome showed that ZmASR1 was the major expressed isoform in leaves and kernels. We found that up-regulation by drought in leaves was a common feature to all ZmASRs, except ZmASR3, in contrast to kernels where only ZmASR2 transcript levels increased. Transgenic maize plants over-expressing ZmASR1 (ZmASR1-OE) displayed increased shoot biomass yield under fully irrigated condition and increased ear leaf area, kernel yield weight and kernel number under both fully irrigated and water-limited conditions in the field. Comparative transcriptomic and proteomic analyses of ZmASR1-OE and wild-type sister leaves led to the conclusion that ZmASR1-OE triggers small-scale changes on the transcriptional and protein levels that concern mainly genes involved in the raffinose family oligosaccharides or branched-chain amino acid metabolic pathways. Metabolomic analysis confirmed the impact of ZmASR1-OE on these pathways and revealed that ZmASR1-OE decreased the levels of metabolites displaying a negative correlation to biomass in Arabidopsis. Collectively, these data demonstrate the feasibility of engineering drought stress tolerance and yield new insights into the function of the ZmASR1 protein. S02-001: DISSECTING THE MOLECULAR REGULA- TION OF CORK CAMBIUM Miguel, A.¹* - Milhinhos, A.¹ - Pinto Ricardo, C.² - Jones, B.³ - Miguel, C.¹ ¹IBET/ITQB-UNL ²ITQB-UNL ³University of Sydney-Australia/UPSC-Sweden *Corresponding author e-mail: andreiamiguel@itqb.unl.pt In plant stems, the cork cambium usually initiates in the subepidermis. Cork cambium cells divide periclinally, giving rise to phelloderm cells on the inside and to phellem (cork) cells on the outside. Together, the cork cambium, cork and phelloderm form the periderm which protects the live tissues from damaging factors. Cork extracted from the cork oak is also a renewable material of high economic value due to its unique properties. Our major goal is to identify molecular regulators of this lateral meristem and we are presently focusing on the role of a few transcription factors, namely SHORT-ROOT (SHR) from the GRAS family. This gene has been extensively studied in the Arabidopsis root where it is described as a key component in the developmental pathway regulating the specification of the root stem cell niche and radial patterning (1, 2, 3). Through an integrated approach combining cell biology and gene expression characterization tools we aim to provide evidence of the putative involvement of SHR in the regulation of cork cambium. SHR sequences have been cloned from poplar and cork oak transcriptome and the effects of down-regulation and ectopic expression of SHR in transgenic poplar lines are being investigated. A comparative analysis of SHR expression patterns in poplar and cork oak will be conducted in order to validate the use of poplar as a model for these studies. References: 1. Benfey PN, et al. (1993) Development 119: 57-70; 2. Helariutta Y, et al. (2000) Cell 101: 555-567; 3. Nakajima K, et al. (2001) Nature 413: 307-311. Acknowledgments: Financial support provided by FCT through project PTDC/AGRGPL/ 098369/2008 and grant SFRH/ BD/44474/2008. S02-002: INSIGHTS IN THE DISCOVERY OF NOVEL RE- GULATORS THAT CONTROL PLANT ARCHITECTURE Alos Ros, E.* - Wigge, P. - Kumar, V. - Lucyshyn, D. John Innes Cente *Corresponding author e-mail: enriqueta.alos@bbsrc.ac.uk In Arabidopsis, a key role in maintaining indeterminate vegetative meristems is carried out by a molecule closely related to FLOWERING LOCUS T (FT), TERMINAL FLOWER 1 (TFL1), that acts in combination ith FD to repress expression of flowering genes. While mutants that lack FT are very late flowering, tfl1 mutations have the opposite phenotype, causing the plants to flower early and terminate their growth with a profusion of flowers, resembling gain-of-function plants (1, 2, 3). To address both plant architecture and the limiting role of FT in plant development, we have performed a genetic screen for flowering time and architecture mutants in the - 1 background. 3400 M2 families have been screened and 20 mutants selected as modifiers of the tfl1-1 phenotype. Amongst the isolated mutants, mutations in already described genes have been identified such as APETALA 1 and LEAFY. Interestingly, novel modifiers of the tfl1-1 phenotype have been obtained and mapped. The functional characterization and the roles of these genes in plant architecture are discussed in the present work. 1. Bradley, D., Ratcliffe, O., Vincent, C., Carpenter, R., and Coen, E. (1997). Science 275, 80-83. 2. Kardailsky, I., Shukla, V.K., Ahn, J.H., Dagenais, N., Christensen, S.K., Nguyen, J.T., Chory, J., Harrison, M.J., and Weigel, D. (1999). Science 286, 1962-1965. 3. Prusinkiewicz, P., Erasmus, Y., Lane, B., Harder, L.D., and Coen, E. (2007). Science 316, 1452-1456. S02-003: CLOCK-MEDIATED CONTROL OF GIBBERE- LLIN RESPONSES IN ARABIDOPSIS Arana, M. V. ¹* - Marín, Nora A.¹ - Maloof, J. N.² - Alabadi, D.¹ - Blázquez, M.A. ¹IBMCP - Universidad Politécnica de Valencia. Valencia. España ²Section of Plant Biology, College of Biological Sciences, University of California, Davis *Corresponding author e-mail: arana@agro.uba.ar The circadian clock acts as central coordinator of plant activity, and it regulates key traits for plant fitness such as seed germination, gas exchange, flowering and growth. Particularly, growth of germinating seedlings is restricted to certain times of the day, showing a maximum rate near dawn. This pattern can be explained by the lightmediated degradation of PIF proteins during the day combined with the clock-mediated repression of PIF transcript accumulation early in the night. This combined action yields high levels of PIF proteins at the end of the night, which are responsible for growth. In addition to this mechanism, growth is controlled by several plant hormones such as gibberellins (GA), auxins and brassinosteroids, and GA action has been shown to involve the relief of the DELLA-mediated inhibition of PIF transcriptional activity. Although GA signaling has been thoroughly studied in constant environments its contribution to plant growth under predictable daily environmental changes such as day/night cycles is still unknown. Here we show that the circadian clock gates hypocotyl GA sensitivity, resulting in the promotion of GA signaling late in the night, the moment when maximum growth occurs. This effect involves the transcriptional control of the GA receptors and influences both the daily patterns of DELLA accumulation and the rate of hypocotyl elongation. Taken together, our results show that anticipation of biological events to external day and night cycles requires a functional GA signaling, and that the GA pathway is a relevant clock output for the control early plant developmental traits, such as daily growth rhythms. S02-004: DEVELOPMENT AND VALIDATION OF A SEN- SITIVE AND RAPID METHOD FOR THE DETERMINA- TION OF UNLABELED AND DEUTERIUM LABELED PLANT HORMONES IN DIFFERENT PLANT TISSUES USING UPLC-MS/MS Müller, M. * - Munné-Bosch, S. University of Barcelona *Corresponding author e-mail: maren.muller@ub.edu Plant hormones play a pivotal role in several physiological processes during a plant’s life cycle, from germination to senes-
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Author Index Aarts, M.G. S18-002 Ab
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Cattivelli, L. P01-031 Cawly, J. P1
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Gallego, B. P17-037 Gallego, P.P. P
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Kersten, B. P10-017 Keskin, O. P05-
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Miguel, C. S02-001, P01-122 Milhinh
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Ramiro, M. P09-018 Ramon, M P13-002
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Tanimoto, E. P01-045 Tarakanov, I.
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