Book of Abstracts - Geyseco

More documents

Recommendations

Info

P - Posters fic responses and allows the establishment of relations between some cell responses and environmental stress tolerance, i.e. osmotic adjustment, proline accumulation or membrane transport (Luttset al. JPP 149: 186-95, 1996; Kerkeb et al. PPlant 116: 37- 41, 2002; Yang et al. PC Rep 26: 229-35, 2007). We have further developed this experimental system by the use of cell lines subjected to dehydration stresses of different magnitude and selecting those with high or low tolerance to compare their responses to stress. Thus, we studied putative physiological and genomic variations related to cryopreservation procedures (Moukadiri et al. PPlant 105: 442-9, 1999), the pattern of endocytosis under osmotic and saline stress (Bahaji et al. PCP 44: 1100-11, 2003), and demonstrated the relationship between tolerance and ability of ABA synthesis under stress (Perales et al. PPB 43: 786-92, 2005). More recently, we used transformed rice cell lines to study the induction of a poliubiquitin promoter by dehydration stresses (Perales et al JPP 165: 159-71, 2008) and, at present, we are investigating the involvement of (plasmalemma and tonoplast) H + pumps in NaCl tolerance mechanisms (Pons et al, MS in prep.). Results from this investigation will be presented and discussed in relation to previous research performed with this experimental system. P01-050: CHANGES IN FREE AND CONJUGATED ABS- CISIC ACID (ABA AND ABAGE) CONCENTRATIONS IN FIVE CULTIVARS OF HORDEUM VULGARE PLANTS UNDER WATER STRESS CONDITIONS Thameur, A. 1 - Ferchichi, A. 1 - López-Carbonell, M. 2 1 Institue Arid Zones, Medenine, Tunisie 2 University of Barcelona- Department of Plant Biology Changes in endogenous abscisic acid (ABA) and its glucose ester conjugate (ABAGE) as well as in proline content, water relations and growth parameters in five barley genotypes (Ardhaoui, Manel, Pakistan, Roho, Rihane) with different drought resistance characteristics have been studied. The aim of this work was to study the balance between free and conjugate ABA and how it can affect the development of these plants grown under water deficit conditions. Differences among the five genotypes lead to changes in the pattern of growth and development. Water stress led to a reduction in relative water content, as well as an increase in proline and endogenous ABA and ABAGE concentrations in all tested genotypes. The increase of proline ranged between 2-fold for var. Rihane and 1.3 fold for var. Manel. The lack of water lead to increase in endogenous ABA concentrations between 5-fold for cv. Ardhaoui and 1.4-fold for var. Roho. Also small increases in endogenous conjugated ABA in all genotypes, except for cv. Ardhaoui, were observed. Nevertheless, the increases in free ABA were more pronounced in tolerant cultivars than in the less tolerant ones. Our results show that changes in growth parameters were correlated with variations of endogenous concentrations of ABA and ABAGE, and will contribute to the knowledge of the involved mechanisms in drought adaptation of Hordeum vulgare plants. P01-051: LONG-TERM RESPONSES TO WATER STRESS IN SELECTED CLONES OF EUCALYPTUS GLOBULUS L. Granda, V. 1 * - de la Torre, C. 1 - Feito, I. 2 - Cuesta, C. 1 - Hernández, B. 1 - Kidelman, A. 2 - Majada, J. 2 - Ordás, R.J. 1 - Rodriguez, A. 1 1 Departamento de Biología de Organismos y Sistemas, Área de Fisiología Vegetal. Universidad de Oviedo 2 Área de Cultivos Hortofrutícolas y Forestales, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA) , Asturias. *Corresponding author, e-mail: biovgg@gmail.com The response of juvenile forest crop plants to water stress is key to the survival of forest populations. In this stage of development plants are very sensitive to water stress; this in fact being the major source of plant loss in commercial plantations. Therefore early detection of tolerant genotypes/varieties using physiological markers would be useful to avoid major loss during field establishment of plantations.In order to understand the physiological changes and adaptation occurring in juvenile plants of Eucalyptus globulus L., selected clones were subjected to long-term stress in controlled conditions. Half of the plants of every clone were maintained at 90-100% of field water capacity, whereas the water supply to the remainder was reduced; firstly to 40% of field capacity (first sampling), and finally, until the death of the plant (final sampling). Plants were monitored throughout the experiment to detect changes in foliar area and growth. Hydrical, photosynthetic, metabolic and other physiological measures were also taken to compare the behavior of the different clones and the establishment of physiological markers for water stress tolerant clones.Acknowledgements. This work was supported by the projects of Ministerio de Educación (AGL2006-13912-C02-01 and GEN2006-27791-C2-1E/VEG). V. Granda is funded by a predoctoral grant by Ministerio de Educación y Ciencia (FPI BES-2007-15663). The eucalyptus clones were provided by ENCE group. P01-052: TOMATO NHX ANTIPORTERS Galvez, F.J. - Cagnac, O. - Abushamsiya, K. - Rodríguez Rosales, M.P. - Venema, K. EEZ-CSIC Plant NHX antiporters can be subdivided in tonoplast Class I proteins, with a suggested role in salt tolerance by Na + accumulation in vacuoles, and Class II proteins. We previously determined an endosomal localization of the Class II LeNHX2 protein in plants and confirmed this expression pattern in yeast. We also determined a predominantly K + /H + exchange reaction of the protein in vitro. These data suggest that the salt tolerance phenotype in over-expressing yeast does not rely strictly on vacuolar Na+ accumulation, which we confirmed in transgenic tomato. We have identified several more tomato NHX isoforms. The expression of the isoforms was induced more by salt stress in salt tolerant tomato species as compared to salt-sensitive species, confirming that NHX genes are determinants of salt tolerance. Of these isoforms, Class I LeNHX4 shows a high expression level in Fruit and Flower tissue, and could thus play an important role in K + accumulation in these tissues, essential during flowering and fruit development. Like LeNHX2, LeNHX4 confers salt, KCl and Hygromycin resistance to yeast with a disruption in the yeast ScNHX1 gene. The LeNHX4 protein has a tonoplast localization in tomato plants, as is to be expected for a Class I NHX protein. In yeast the majority of the fluorescence signal from GFP tagged protein is found inside the vacuoles, and not in the membrane, pointing to a degradation of the protein. This is also indicated by SDS-PAGE electrophoresis, were the protein appeared as a small band of the expected molecular weight and a large amount of smaller molecular weight. As a consequence, we could so far not determine the antiporter activity in vitro. New results regarding function and functioning of the LeNHX4 protein will be presented P01-053: AGEING AND IRRADIANCE ENHANCE VITA- MIN E CONTENT IN GREEN EDIBLE TISSUES FROM CROP PLANTS Garcia Plazaola, J.* - Lizarazo, K. - Fernández-Marín, B. - Becerril, J.M. Universidad del País Vasco *Corresponding author, e-mail: joseignacio.garcia@ehu.es Tocopherol (vitamin E) is an antioxidant essential in human nutrition. Several approaches have aimed to enhance tocopherol content in crops by the genetic modification of plants, a practice that generates some social concern. As tocopherol accumulates with leaf age in some wild plants and the antioxidant mechanisms respond plastically to stress conditions, we hypothesize P
FESPB 2010 - XVII Congress of the Federation of European Societies of Plant Biology in this study that tocopherol content can be increased in edible plants by the manipulation of harvesting time and growth conditions, in particular irradiance. We have studied ontogenic changes in tocopherol concentration in photosynthetic tissues of edible leaves (lettuce, spinach, corn salad and dandelion) and green fruits (cucumber and pepper). In all species tocopherol content increased with tissue age. Spinach showed the fastest rate of tocopherol accumulation, and the growth at higher irradiance had a synergistic effect over the rates of accumulation. The same irradiance dependency of this accumulation was observed in fruits, but a final decrease with senescence occurred in cucumber. This study demonstrates that the content of tocopherol in vegetables can be notably enhanced (or reduced) by simply selecting the adequate harvesting time and/or by manipulating the environmental conditions during the growth period P01-054: PHYTOREMEDIATION CAPABILITY OF BRASSICA NAPUS GROWN ON SOILS, CONTAMINA- TED WITH HEAVY METALS Koshkin, E.* - Vagun, I. Russian State Agrarian University-Moscow Timiryazev Agricultural Academy Summer oilseed (Brassica napus L.) variety Podmoskovniy was grawn in a greenhouse in a pot culture with sod-podzol soil contaminated (in mg/kg soil) with Pb (from 50 up to 400 in every 50 mg/kg dosage interval), Cd (from 2 up to 14 in every 2 mg/ kg dosage interval) and Zn (from 100 up to 800 in every 100 mg/ kg dosage interval). Linear relationship was established between Zn and Pb accumulation in shoots at seed maturity stage and their concentrations in aboveground biomass. As for Cd, linear relations between above parameters exists only up to its concentration of about 15 mg/kg DW and reached a plateau there after. Harvest index (Hi) decreased with increasing Zn and Pb concentration in the seeds and remained almost constant with Cd.With increasing rates of heavy metals (HM) contamination the ratio of their accumulation in the seeds to that in shoots decreased for Pb and Zn and remained almost stable for Cd. Accumulation of Pb and Cd in shoots increased with increasing HM concentration in seeds. No regular pattern was established for Zn due to reverse relationship between its concentration in seeds and shoot DM accumulation. P01-055: NA + -ATPASES IN MARINE GREEN MICROAL- GAE. Popova, L.* - Balnokin, Y. K.A.Timiryazev Institute of Plant physiology, Russian Academy of Sciences *Corresponding author, e-mail: lora_gp@mail.ru All organisms surviving high substrate salinity prevent excessive Na + accumulation in cytoplasm due to operation of Na + -translocating proteins localized to the plasma membrane/tonoplast and exported Na + from the cytoplasm to the external medium/ vacuole. A wide diversity of the enzymes executing primarily active sodium export from cells operates in cell membrane of prokaryotes. In eukaryotes, only P-type ATPases resided in plasma membranes mediate primarily active sodium export from cells. Mammalian Na + ,K + -ATPase is the first and most extensively studied representative of this ATPase family. More recently, Na+-translocating ATPases of P-type were also discovered in marine golden-brown microalga Heterosigma akashiwo (the kingdom Chromista) (Wada et al., 1992) and some yeast species (the kingdom Fungi) (Benito et al., 2002). In halotolerant plants the existence of a primary Na+-transporter was debated for a long time. Nevertheless, primary Na+-pumps have been found in some representatives of the kingdom Plantae. Na + -ATPases of P-type were found in two marine green microalga species, Tetraselmis viridis (Balnokin and Popova, 1995) and Dunaliella maritima (Popova et al., 2005). Both species belong to the class Prasinophyceae which may be a paraphyletic basal group to all green plants. The Na + -ATPases from the algae demonstrate near similarity. Both ATPases are electrogenic enzymes and operate in the weakly alkaline pH range with maximal activity at pH 7.5 – 8.0. They are highly specific to Na + and could not transfer K + thus differing from both animal-type Na + ,K + -ATPase that exchanges Na + for K + and fungal-type Na + -ATPase that does not discriminate between Na + or K + (Benito et al., 2002). P01-056: TEMPERATURE DROP APPLIED AT EARLY STAGES OF ONTOGENESIS CAN ENHANCE PLANT DEVELOPMENT Sysoeva, M.* - Markovskaya, E. - Sherudilo, E. - Shibaeva, T. Institute of Biology, Karelian Research Centre RAS *Corresponding author, e-mail: sysoeva@krc.karelia.ru It is well known that a short duration temperature drop may affect plant morphogenesis. However, limited data are available on its effect on plant development. The experiments were conducted with different plant species: cucumber, cabbage, marigold, pansy, petunia. Seeds or plants at early stages of ontogenesis were treated with temperature drops for 6-7 days. The intensity and duration of the temperature drop varied with plant species. Temperature drop increased the number of leaves in cucumber young plants and cabbage, accelerated flowering and improved plant quality in marigold but not in petunia. Pre-sowing seed treatment with temperature drop has also hastened flowering in marigold and pansy. Possible underlying mechanisms which contribute to these effects will be discussed. The study was supported financially by the Russian Foundation for Basic Research (N 07-04-00063). P01-057: CHANGES IN CHLOROPLAST LIPOXYGENA- SE 6 LEVEL AND LOCALIZATION UNDER DARK-CHI- LLING CONDITIONS IN COMMON BEAN (PHASEO- LUS VULGARIS L.) Rudowska, L. 1 * - Mazur, R. 1 - Rumak, I. 1 - Kozlowski, P. 1 - Trzcinska-Danielewicz, J. 1 - Hapka, M. 1 - Michalski, W. 2 - Mostowska, A. 1 - Garstka M. 1 1 University of Warsaw 2 CSIRO Australian Animal Health Laboratory *Corresponding author, e-mail: lucja.rudo@gmail.com It was demonstrated that low temperature induced changes in the chloroplast structure and function of chilling sensitive (CS) plant species. These changes were due to the rearrangement of chlorophyll-protein complexes inside the thylakoid membranes. We found out that in CS Common bean (Phaseolus vulgaris L.) the dark-chilling stress induces association of the lipoxygenase 6 (LOX6) with the thylakoid membranes. LOX6 is probably involved in the oxylipin synthesis against wounding and non-host pathogen infection. For detailed analysis of LOX6 we used both molecular (immunodetection, mass spectrometry and northernblot) and microscopy (electron microscopy with immunogold labeling) techniques. Our analysis has shown increased PvLOX6 mRNA and LOX6 protein levels in thylakoids during dark-chilling. Furthermore we have observed reverse changes in LOX6 molecular weight. Microscope images have conformed the chloroplast localization of bean lipoxygenases. More than half of the gold particles for LOX proteins were localized in the thylakoid membranes in all experimental variants. We have observed that there are differences in specific localization of the LOX proteins in the thylakoid and granum compartments. P01-058: ECO-PHYSIOLOGICAL TRAITS AND POLY(ADP-RIBOSYL)ATION ACTIVITY IN WINTER AND SUMMER LEAVES OF THE MEDITERRANEAN SPECIES CISTUS INCANUS L.
Page 4: FESPB 2010 - XVII Congress of the F
Page 7 and 8: PLENAR SESSION LECTUR
Page 9 and 10: FESPB 2010 - XVII Congress of the F
Page 13 and 14: PARALL SESSION LECTUR
Page 39 and 40: POST 04 POSTERS • Environmental S
Page 51: FESPB 2010 - XVII Congress of the F
Page 103 and 104:
FESPB 2010 - XVII Congress of the F
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 206 and 207:
Author Index Aarts, M.G. S18-002 Ab
Page 208 and 209:
Cattivelli, L. P01-031 Cawly, J. P1
Page 210 and 211:
Gallego, B. P17-037 Gallego, P.P. P
Page 212 and 213:
Kersten, B. P10-017 Keskin, O. P05-
Page 214 and 215:
Miguel, C. S02-001, P01-122 Milhinh
Page 216 and 217:
Ramiro, M. P09-018 Ramon, M P13-002
Page 218 and 219:
Tanimoto, E. P01-045 Tarakanov, I.
show all

Book of Abstracts - Geyseco

Create successful ePaper yourself

Delete template?

Save as template?