Book of Abstracts - Geyseco

More documents

Recommendations

Info

P - Posters activated by phosphorylation of its penultimate residue, a threonine and the consecutive binding of regulatory 14-3-3 proteins to the enzyme C-ter. Mass spectrometry analysis of purified PMA2 (Plasma membrane H+-ATPases from N. plumbaginifolia) led to the identification of new phosphorylation sites. Phosphorylated Ser938 and Thr931, both located in the PMA2 14-3-3 binding site, were shown to act as negative regulators of the 14-3-3 binding and hence the enzyme activation whereas phosphorylated PMA2 Thr889, also located in the enzyme C-ter but outside the 14-3-3 binding site, seemed to be involved in an activation mechanism independent of the 14-3-3 protein binding. Altogether 4 phosphorylated sites concur to a complex regulation of the H+- ATPase. To identify the kinases involved, proteins co-purified with a His-tagged PMA2 isoform expressed in tobacco BY2 suspension cells were analyzed by mass spectrometry. Some putative kinases have been identified. To better characterize the physiological roles of the H+-ATPase in the plant, an activated enzyme was expressed in tobacco and Arabidopsis. Transgenic plants had a pleiotropic phenotype with, for example, a modified development and a better resistance to salt stress and basic pH. P11-018: PATTERN FORMATION DURING SOMATIC EMBRYOGENESIS IN SCOTS PINE Abrahamsson, M.* - Valladares, S. - von Arnold, S. Swedish University of Agricultural Sciences, Department of Plant Biology and Forest Genetics *Corresponding author, e-mail: malin.abrahamsson@vbsg.slu.se Somatic embryogenesis is an attractive method to propagate conifers vegetatively. However, in order to efficiently regulate the formation of plants via somatic embryos it is important to understand how the somatic embryos develop. The aim of this study has been both to elucidate the development of somatic embryos in Scots pine and to identify deviations from the normal plan leading to developmental arrest or to progressive accumulation of errors resulting in aberrant cotyledonary embryos. We have compared the developmental pathway of somatic embryogenesis in representative cell lines yielding cotyledonary embryos with normal and abnormal morphology. Embryogenic cultures of Scots pine are initiated from immature embryos during the cleavage phase, and proliferation by cleavage can also be observed in embryogenic cultures. In all cell lines a large proportion of the developing embryos degenerate but the degeneration pattern differs among cell lines. However, there were no fundamental differences in the early patterning of embryos between the cell lines except that the early somatic embryos in cell lines giving rise to abnormal embryos carried supernumerary suspensor cells, resulting in an unbalanced ratio between the embryonal mass and the suspensor, which partly can be explained by an aberrant polar auxin transport. P11-019: ISOLATION AND CHARACTERIZATION OF ENDOSOMAL COMPARTMENTS IN ARABIDOPSIS Sancho Andrés, G.* - Groen, A.J. - Lilley, K.S. - Aniento, F. Universidad de Valencia *Corresponding author, e-mail: gloria.sancho@uv.es The plasma membrane (PM) of plant cells undergoes dynamic changes in protein composition. Several PM proteins have been shown to cycle between the PM and endomembrane compartment(s), whereas other PM proteins are internalised and targeted to the vacuole for degradation. PM protein dynamics thus determines cell behaviour and affects plant performance. However, our current knowledge of the underlying mechanisms of these processes in plants is virtually non-existent. In animals, early/sorting endosomes are important sites for receptor signaling. Although this may also apply to plants, there are no markers to distinguish plant early/sorting from recycling endosomes. The endosomal compartments in which plant PM proteins are sorted for degradation or recycling to the PM are morphologically and functionally not defined, and their composition in terms of resident and cargo proteins is essentially unknown. In this work, we have carried out two proteomics methods to characterise endosomal compartments in Arabidopsis. Firstlyw, e have combined subcellular fractionation with LOPIT (Localization of Organelle Proteins by Isotope Tagging), a method which allows assignment of proteins to organelles using high throughput quantitative proteomics approaches. Secondly, in parallel, we have performed immunoisolation experiments, using as antigen the human transferrin receptor (hTfR), a model receptor for endocytosis in animal cells, heterologously expressed in transgenic Arabidopsis plants. P11-020: NOVEL INSIGHTS INTO AQUAPORIN TRA- FFICKING TO THE PLASMA MEMBRANE Besserer, A. - Bienert Gerd, P. - Chevalier, A. - Zelazny, E. - Chaumont, F. Institut des sciences de la vie, Université catholique de Louvain The movement of water across plant plasma membrane (PM) depends on the amount and activity of aquaporins belonging to the Plasma membrane Intrinsic Proteins (PIP) subfamily. Recently, we showed that maize aquaporins belonging to PIP1 and PIP2 groups form hetero-oligomers when co-expressed in leaf mesophyll protoplasts. This physical interaction regulates their trafficking and triggers relocalization of ZmPIP1 from the endoplasmic reticulum (ER) to the PM (Zelazny et al., 2007;2009). This result suggests that ZmPIP1s carry ER retention signals which are inefficient upon hetero-oligomerization. Expression of mutated and chimeric PIPs indicates that the loop A of ZmPIP1;2 may contain an ER retention signal as its replacement with ZmPIP2,5 loop A leads to some extent to ZmPIP1;2 trafficking to the PM, but only if an additional ER export motif (N-terminal diacidic acid motif) is present. Regulation of PIP trafficking to the PM was further characterized in maize protoplast and in tobacco epidermal cells by co-expressing ZmPIP2;5 and the dominant negative mutant syntaxin SYP121-sp2. Our results showed that ZmPIP2;5 traffic to the PM is hampered by SYP121-sp2. Putative interaction between SYP121 and ZmPIP2;5 is under investigation. The membrane osmotic water permeability decreased in cells co-expressing ZmPIP2;5 and SYP121-sp2. Altogether data point toward a complex and highly integrated regulation of PIP trafficking in the maintenance of cellular water homeostasis. P11-021: COMPARISION OF GALACTOGLUCOMAN- NAN OLIGOSACCHARIDES ACTION ON CELL ELON- GATION IN HYPOCOTYL AND PRIMARY ROOT Richterová, D.* - Kollárová, K. - Lišková, D. Institute of Chemistry, Slovak Academy of Sciences *Corresponding author, e-mail: danica.richterova@savba.sk Galactoglucomannan oligosaccharides (GGMOs) inhibit the auxins-induced elongation growth of stem segments and this effect is dependent on their chemical structure and concentration. GGMOs influenced induction and elongation of adventitious and lateral roots. The aim of this work was to answer the question: is the effect of GGMOs in elongating hypocotyls and roots related with the elongation or division of cells, and in which tissues? GGMOs were derived from spruce galactoglucomannan. Modified GGMOs - GGMOs-g were prepared by treatment of GGMOs with purified α-galactosidase. Uniform seedings of mung bean (Vigna radiata (L.) Wilczek) were transferred to hydroponic Hoagland solution containing GGMOs or GGMOs-g alone and/ or in combination with IBA. Plants were grown 7 days in controlled conditions and then the length of hypocotyl and primary root was measured. For light microscopy the whole-mount procedure was used and the samples were stained with toluidine blue. The length of cells was determined by Lucia analysis system. The data were analyzed using ANOVA. GGMOs alone or in combination with IBA inhibited hypocotyl elongation, but they stimu- P
FESPB 2010 - XVII Congress of the Federation of European Societies of Plant Biology lated primary root growth. However, distinct effect of GGMOs-g compared with GGMOs has been observed, and a different way of elongation in single tissues after GGMOs treatment has been determined (in the epidermis or rhizodermis, and in the primary cortex). The hypocotyl or root elongation growth induced by GGMOs was related to epidermal/rhizodermal cells length and primary cortical cells division. GGMOs didn’t affect the length of primary cortical cells compared with IBA or the control. Acknowledgement: This work was supported by grants - VEGA 1/0472/10, 2/0046/10 and APVT 51-013304. P11-022: DISTINCT POPLAR DEFENSE STRATEGIES AGAINST HERBIVORES REQUIRE DIFFERENT TYPES OF EXTRAFLORAL NECTAR SECRETION Escalante-Pérez, M. 1 * - Reinders, J. 2 - Lautner, S. 3 - Fromm, J. 3 - Hedrich, R. 1 - Ache, P. 1 1 University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften 2 University Regensburg, Institute of Functional Genomics 3 University Hamburg, Zentrum Holzwirtschaf *Corresponding author, e-mail: escalante@botanik.uni-wuerzburg.de A large number of plant species grow extrafloral nectaries and produce nectar to attract predators such as ants to defend themselves against herbivores. We studied how insect feeding feeds back on nectary development and activity in Populus. Thereby we analyzed nectaries anatomy, ecology, gene expression and nectar chemistry from Populus trichocarpa and P. tremula x P. tremuloides (Ptt). Both vary widely in morphology and structure and thus differ in the type of nectar secretion which leads to different defense strategies. While in Ptt the presence of nectaries is constitutive, nectary appearance in P. trichocarpa is strictly inducible. Simulating insects foraging with P.trichocarpa we could demonstrate that wounding induces formation of non-secreting nectaries, while nectar production requires the involvement of an herbivore delivered elicitor. In line with these findings, microarray analysis of Ptt nectaries vs. leaves revealed up-regulation of genes involved in hormone action, stress, cell wall and sugar metabolism. Ptt nectar is very likely released via exocytosis. Consequently genes involved in lipid metabolism and secretion are also induced in nectaries. P11-023: COMMON PLAYERS IN ORGANELLES DIVI- SION PROCESSES: MORPHOLOGICAL AND MOLECU- LAR ANALYSIS IN PLANT Ruberti, C.* - Costa, A. - Zottini, M. Dipartimento di Biologia, Università degli Studi di Padova, Italy *Corresponding author, e-mail: cristina.ruberti@gmail.com Fiorella Lo Schiavo (Dipartimento di Biologia, Università degli Studi di Padova, Via U. Bassi 58/B, 35131 Padova, Italy. ) Mitochondria and peroxisomes are highly dynamic organelles with a large plasticity in their shape and morphology. In particular, recent reports show that mitochondrial morphology changes during plant senescence. Studies in mammals, fungi and plants have led to the finding that mitochondria and peroxisomes partially share components of their division machinery, such as dynamin-like and fission-like proteins. In Arabidopsis, dynamin-like proteins DRP3A and DRP3B and the fission like protein BIGYIN have been implicated in mitochondrial and peroxisome fission. In addition, another specific plant factor ELM1 has been recently reported to be involved in mitochondria fission. In order to gain inside the molecular mechanisms involved in the remodelling of organelles we analysed throughout the plant development the expression pattern and the subcellular localization of BIGYIN and ELM1. To this aim Arabidopsis transgenic plants expressing the GUS reporter gene under the control of the BIGYIN and ELM1 promoters, and plants transformed with pBIGYIN-YFP:BIGYIN construct have been generated. Moreover, BIGYIN and ELM1 fused to different fluorescent proteins (YFP and DsRed2) were transiently co-expressed in Arabidopsis mesophyll protoplasts. The same system was also employed to test the in vivo proteinprotein interaction by means of bimolecular fluorescent complementation. Our data show that ELM1 and BIGYIN have a highly specific expression pattern in the plant, and that their subcellular localization is not restricted to the subcellular compartments previously described. P11-024: RECRUITMENT OF GLUTATHIONE INTO THE NUCLEUS DURING CELL PROLIFERATION IN ARABI- DOPSIS THALIANA Foyer, C.H. 1 - Diaz Vivancos, P. 2 - Pellny, T.K. 3 - Markovic, J. 4 - Pallardó, F.V. 4 1 University of Leeds 2 CEBAS-CSIC 3 Rothamsted Research 4 University of Valencia-CIBERER Cellular redox homeostasis is considered to be important in the regulation of cell proliferation but little information is available on how redox control regulates the cell cycle or regarding the precise functions of key redox metabolites. The intracellular redox state of Arabidopsis cells is modulated during proliferation by interplay between the pyridine nucleotides, glutathione and ascorbate pools. Evidence of similarities in the redox control of cell proliferation in animals and plants will provided. For example, GSH is recruited into the nucleus early in cell proliferation in Arabidopsis thaliana. GSH accumulation in the nucleus was triggered by treatments that synchronize cells at G1/S as identified by flow cytometry and marker transcripts. Significant decreases in transcripts associated with oxidative signaling and stress tolerance occurred when GSH was localized in the nucleus. Increases in GSH1 and GSH2 transcripts accompanied the large increase in total cellular GSH observed during cell proliferation, but only GSH2 was differentially expressed in cells with high GSHn relative to those with an even intracellular distribution of GSH. Of the 7 Bcl-2 associated (BAG) genes in A. thaliana, only the nuclear-localized BAG 6 was differentially expressed in cells with high GSHn compared to GSHc. We conclude that GSHn is associated with decreased oxidative signaling and stress responses and that whole cell redox homeostasis is restored as the cell cycle progresses by enhanced GSH synthesis and accumulation in the cytoplasm. P11-025: THE EFFECTS OF LOW AND HIGH TEMPERA- TURES ON ULTRASTRUCTURE OF BRASSICA CAM- PESTRIS AND AMARANTHUS CAUDATHUS LEAVE CELLS Klymchuk, D.* - Vorobyova, T. - Kosakivska, I. M.G. Kholodny Institute of Botany, NAS of Ukraine *Corresponding author, e-mail: microscopy@botany.kiev.ua The effects of low and high temperatures on the subcellular structure of mesophyll and bundle sheath cells in leaves of Brassica саmpestris var. olifera and Amaranthus caudathus L. belonging to plants with C3 and C4 carbon fixation, respectively, were evaluated. Plants were grown under regime 15 h light (5500±500 lx) and 9 h dark at a temperature 24±1ºC. 25-day-old plants during dark period were subjected to low positive (4ºC) and high (40ºC) temperatures for 2 h. Leaf samples taken from the middle region of the true leaves were fixed and cross sectioned (50-60 nm) for TEM analysis. Low and high temperatures decreased the volume of chloroplast starch granules, altered the amount of plastoglobuli per chloroplast and the amount cytoplasmic lipid drops in mesophyll and bundle sheath cells from both plants. Additionally, high temperature induced the alteration in mitochondrion inner and cytoplasmic membrane structures in mesophyll cells from both plants. These data suggest that short-term temperature stresses influenced first of all chloroplast starch deposition resulted the alteration in their metabolism. The probable roles of ultras-
Page 4:
FESPB 2010 - XVII Congress of the F
Page 7 and 8:
PLENAR SESSION LECTUR
Page 9 and 10:
Page 11 and 12:
Page 13 and 14:
PARALL SESSION LECTUR
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
POST 04 POSTERS • Environmental S
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108: FESPB 2010 - XVII Congress of the F
Page 157: FESPB 2010 - XVII Congress of the F
Page 206 and 207: Author Index Aarts, M.G. S18-002 Ab
Page 208 and 209:
Cattivelli, L. P01-031 Cawly, J. P1
Page 210 and 211:
Gallego, B. P17-037 Gallego, P.P. P
Page 212 and 213:
Kersten, B. P10-017 Keskin, O. P05-
Page 214 and 215:
Miguel, C. S02-001, P01-122 Milhinh
Page 216 and 217:
Ramiro, M. P09-018 Ramon, M P13-002
Page 218 and 219:
Tanimoto, E. P01-045 Tarakanov, I.
show all

Book of Abstracts - Geyseco

Create successful ePaper yourself

Delete template?

Save as template?