Book of Abstracts - Geyseco

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P - Posters (β RNAi / WT plants) and d1 (β RNAi / d1 plants) by RNAi method. β RNAi / WT plants showed the abnormal phenotype, namely dwarf, small seed, browning of lamina joint region and node, and decreased fertility. Almost all abnormality that β RNAi / WT plants showed was different from that of d1. Western blot analyses using anti-Gβ antibody indicated that the amount of the β subunit in some β RNAi / WT plants reduced about 30%, compared with wild type.β RNAi / d1 plants showed the abnormal phenotype, namely dwarf, small seed, browning of lamina joint region and node, and decreased fertility. Western blot analyses using anti-Gβ antibody indicated that the amount of the β subunit in some β RNAi / d1 plants reduced about 30%, compared with d1. RNAi / d1 plants showed more severe dwarfism, compared with d1. These results indicate that β subunit has some independent functions, compared with α subunit in rice heterotrimeric G protein. P10-051: 24-EPIBRASSINOLIDE REGULATES CYTOKI- NIN METABOLISM IN WHEAT SEEDLINGS Avalbaev, A.* - Yuldashev, R. - Shakirova, F. - Vysotskaya, L. Russian Academy of Sciences *Corresponding author, e-mail: shakirova@anrb.ru Earlier in our research it was found that influence of 24-epibrassinolide (EB) on wheat seedlings caused rapid and stable double accumulation of cytokinins (CKs). The important contribution to this process had the EB-induced inhibition of gene expression and activity of cytokinin oxidase (CO) which is responsible for cytokinin degradation. Meanwhile, rapid accumulation of isopentenyladenosine and zeatin nucleotide, the primary compounds in the cytokinin biosynthesis, in EB-treated plants suggests that EB might influence on CKs biosynthesis. Probably our results about increase in EB-treated plants of concentration of O-glucosides, the storage forms of active cytokinins, might indicate in favour of this suggestion. Removing of EB from incubation medium of wheat seedlings led to the gradual return of CKs content to the control level. This was accompanied by gradual increase both of CO enzyme activity and level of CO gene expression to the control level. Meanwhile, we have revealed the protective effect of EB on wheat seedlings in response to salinity, and this was connected with the return of salinity-induced decrease in the CKs content to the control level. The received data indicate on the participation of EB in regulation of cytokinin metabolism and important role of endogenous CKs in realization of the physiological action of EB on wheat plants. This work is supported by Grant RFFI 08-04-01563. P10-052: IDENTIFICATION AND FUNCTIONAL CHA- RACTERIZATION OF A BHLH TRANSCRIPTION FACTOR INVOLVED IN THE RIPENING OF FLESHY FRUITS Nicolas, P.* - Lecourieux, F. - Lecourieux, D. - Gomes, E. - Delrot, S. UMR EGFV INRA *Corresponding author, e-mail: Philippe.Nicolas@Bordeaux.Inra.Fr Grape berry development can be divided in two phases: a phase of “herbaceous” growth mediated by cell division and elongation leading to hard and acid green fruits. The second phase corresponds to cell elongation and is characterized by the maturation of the berry, which is characterized by changes in texture and colour. This transition from green stage to ripening is called véraison. From véraison on and during ripening, high amounts of sugars and phenolic compounds accumulate in the berry. In addition to their structural role, sugars can act as a signal able to affect fruit development and ripening. In this context, we identified a new transcription factor, VvbHLH1, which expression is sucrosedependent and berry-specific. Its functional characterisation, supported by overexpression in grape and tomato, suggests that VvbHLH1 may play a key role during berry development and ripening by affecting berry size through modifications of the hormonal balance. P10-053: SE5 SHEDS LIGHT ON THE ROLE OF PHYTO- CHROMES IN PHOTOPERIODIC FLOWERING CON- TROL IN RICE Andres, F. 1 - Talón, M. 2 - Domingo, C. 2 1 Max Planck Institute for Plant Breeding Research 2 Instituto Valenciano de Investigaciones Agrarias A great number of plants synchronize flowering with day length. In rice (Oryza sativa), photoperiod is the primary environmental cue that triggers flowering. Heading is strongly influenced by daylength, promoting flowering under short days. Two independent photoperiod pathways have been defined, one involving Hd1 and the other involving Ehd1, that control heading date by regulating Hd3a, the most important floral integrator. The s73 mutant, identified in a gamma irradiated Bahia collection, displays early flowering and photoperiodic insensitivity due to a null mutation in the SE5 gene, which encodes an enzyme implicated in phytochrome chromophore biosynthesis. Mutations in SE5 cause depletion in the phytochromes function. s73 mutant plants showed a number of alterations in the characteristic diurnal expression patterns of master genes involved in photoperiodic control of flowering, resulting in up-regulation of Hd3a. Molecular characterization of s73 provides new insight on the regulation of the photoperiodic control of flowering in rice by showing that phytochromes inhibit flowering affecting both Hd1 and Ehd1 flowering pathways. P10-054: JUB1, A H2O2-REGULATED NAC TRANSCRIP- TION FACTOR, NEGATIVELY CONTROLS SENESCEN- CE AND CONSTITUTES A CENTRAL ELEMENT IN H2O2 SIGNALING Balazadeh, S. 1 - Wu, A. 2 - Allu, A. 3 - Xue, G.P. 4 - Mueller Roeber, B 1 1 University of Potsdam 2 MPI of Molecular Plant Physiology 3 MPI of Molecular Plant Physiology 4 CSIRO Plant Industry Senescence is a genetically controlled process occurring at late stages of development; it can also be induced by abiotic stresses including salinity. A candidate signal mediating age-dependent or abiotic stressinduced senescence is hydrogen peroxide (H2O2). Many transcription factors (TFs) of the NAC family undergo expression changes upon leaf aging. We started to analyze the gene regulatory networks (GRNs) controlled by NACs. Our previous studies indicated that salt-triggered expression of positive senescence regulator ANAC092 and its downstream regulon may at least in part be mediated through a rise of cellular H2O2 level upon salt stress. We now discovered another NAC TF, dubbed JUB1, which functions as a negative regulator of senescence. Its overexpression dampens the intracellular H2O2 level and increases life span accompanied by an increased resistance to oxidative stress. In contrast, precocious senescence and lowered tolerance against abiotic stresses were observed in a jub1-1 knock-down line. JUB1 expression is strongly and rapidly induced by external H2O2. To explore the JUB1 GRN, we determined its preferred binding sites by in vitro binding site selection and performed microarraybased expression profiling using estradiol-inducible JUB1overexpression lines. Based on our results we hypothesize that JUB1 constitutes a central regulator of a finely tuned control system modulating cellular level of H2O2, regulating stress adaptation and the entry into senescence. P
FESPB 2010 - XVII Congress of the Federation of European Societies of Plant Biology P10-055: FUNCTIONAL INTERPLAY OF SMALL RNA NODES AND HORMONES ON PLANT REPRODUCTIVE TRAITS Rubio Somoza, I.* - Detlef, W. Max Planck Institute for Developmental Biology *Corresponding author, e-mail: ignacio.rubio@tuebingen.mpg.de Plant development relies on a natural succession of programs that require a delicate balance among cell division, elongation, differentiation and to a lesser extent cell death. Developmental programs enable the plant for organ patterning and mediate interactions with the surrounding environment. These programs and their progression are orchestrated by a highly dynamic interplay between different plant hormones such as auxin, jasmonic acid (JA) and gibberellic acid (GA). Small RNAs (sRNA) have turn to be virtually involved in all levels of regulation of cellular information networks influencing from the chromatin state to the abundance of transcripts encoding direct regulators of gene expression and protein activity, such as transcriptional factors and F-box proteins. MicroRNAs (miRNAs), a special class of sRNAs, have been linked to the regulation of elements involved in the control of hormone synthesis, homeostasis or transduction in the JA, GA and auxins pathways. We have previously created a comprehensive collection of Arabidopsis lines (MIMIC lines) with specifically reduced activity of a different miRNA family allowing us to interrogate miRNA nodes and their connection within the dynamic hormone context. We will show how two evolutionary unrelated miRNA/target nodes (GA and JA-related) regulate a third node targeting auxin signal transduction elements. This sRNA network mediates the hormone-dependent transition through developmental stages promoting inflorescence vasculature development and maturation of floral organs and establishes reproductive competence. These results show cellular and physiological importance of this network in a hormone context, further reinforcing the role of miRNAs in auxin gradation P10-056: PROTEIN INTERACTORS OF KIN10 Rodrigues, A.* - Duarte, L. - Martinho, C. - Baena-González, E. Instituto Gulbenkian de Ciência *Corresponding author, e-mail: americo@igc.gulbenkian.pt The Arabidopsis SnRK1 protein kinases KIN10 and KIN11 are central regulators of the transcriptome in response to multiple types of stress. Sensing and signaling stress-associated energy deprivation, SnRK1s trigger changes in the expression of over 1000 genes that allow the re-establishment of homeostasis and the mounting of a more specific adaptive response. Despite the importance of SnRK1s for plant growth and acclimation, our current knowledge on this signaling pathway is extremely limited. SnRK1s are the orthologs of the yeast SNF1 and mammalian AMPK, and so far most of the characterization of the SnRK1 system has relied on knowledge from these systems. However, although very valuable and successful, this approach is limited in its ability to uncover novel interactions unique to plants. In order to address this, we are undertaking Y2H screens with three major deviances from earlier screenings: i) we are using libraries generated from a wide range of stress treatments (kindly provided by Prof. John Cushman), ii) screening is performed in parallel also under low-glucose activating conditions and iii) it relies not only on the full-length (FL) SnRK1, but also on the non-conserved C-terminal domain To this end, using FL KIN10 as a bait and clone selection in medium with low and high glucose content, we have identified 59 clones with autotrophic growth in the absence of both adenine and histidine. We are currently confirming these interactions and further investigating the involvement of these components in the SnRK1 pathway. P10-057: TRANSCRIPTOME PROFILING FOR BRASSI- CA NIGRA IN HEAVY METAL TOLERANCE Cevher Keskin, B. 1 * - Yildizhan, Y. 1 - Yuksel, B. 1 - Kulen, O. 1 Memon, A. R. 2 1 TUBITAK Marmara Research Center 2 International University of Sarajevo *Corresponding author, e-mail: birsen.keskin@mam.gov.tr There has been significant interest in the use of plants for the soil remediation contaminated with toxic heavy metals. This technology, termed phytoremediation provides environmentally friendly solutions to use plants to extract heavy metals from the soil and water to accumulate them in shoot tissue. Knowledge of the molecular mechanisms involved in phytoremediation may lead to the development of more efficient plants, and it has been suggested that better phytoremediation management practices could be accomplished by expressing selected genes in more favorable plant species. In this study, Affymetrix GeneChip Arabidopsis Genome Array (ATH1-121501 GeneChip, ATH1; Affymetrix, Santa Clara, CA, USA) was used for the transcriptomal profiling in response to 25 µM Copper. Root and leaf RNAs of B. nigra 6619 (non-tolerant) and B.nigra Diyarbakir (tolerant) ecotypes were hybridized with ATH chips.At the end of microarray experiments, we identified metal transport and accumulation related genes such as; MT1C (Metal Copper Binding Protein), MT2B (Metallothionein 2B, copper ion binding), MT3 (Metallothionein 3) and metal stress related genes (γ-glutamyl cysteine synthetase: γ-ECS, phytochelatin synthase: PC etc). The mRNA expression experiments by real time RT-PCR (qRT-PCR) showed that two enzymes of γ-ECS and PC were highly expressed when plants were subjected to 200 and 500 μM Cu in the growth medium. Quantitative Real Time RT-PCR experiments will be performed to identify other differentially expressed genes. It may be suggested that these genes will serve as molecular tools for the future expression in transgenic plants for the phytoremediation applications. P10-058: MSP DOMAIN-CONTAINING PROTEIN RE- VEALS NEW LEVEL OF REGULATION OF STOMATAL SIGNALING IN ARABIDOPSIS Paciorek, T.* - Abrash, E. - Bergmann, D. Stanford University *Corresponding author, e-mail: paciorek@stanford.edu Stomata are specialized epidermal structures that mediate gas exchange between plant and environment. The formation and patterning of stomatal complexes in Arabidopsis result from molecular interactions within a complex web of functionally interconnected regulators. To date, several components of signal transduction pathway including ligands, receptors and transcriptional factors involved in stomatal patterning have been identified. One of the most crucial regulators of stomatal patterning is the TOO MANY MOUTHS (TMM) gene. TMM encodes a receptor-like protein localized at the plasma membrane in stomatal lineage cells. TMM plays a central role in the deciding whether a cell will enter the stomatal pathway; mutations in this gene result in violation of major patterning rules governing stomatal development. The aim of this study was to identify specific modulators of the TMM receptor. We have designed and performed a forward genetic screen and identified specific suppressors of the tmm-1 mutant. Here, we present molecular characteristics of the MST1 (MSP domain-containing suppressor of tmm 1) gene which specifically modulates the phenotype of tmm but not other stomatal signaling mutants. P10-059: EFFECTS OF THE SAMDC UORF PEPTIDE ON MRNA DEGRADATION AND PROTEIN DEGRADATION Choi, Y.* - Park, K.Y. Sunchon National University *Corresponding author, e-mail: yj1730@sunchon.ac.kr S-adenosylmethionine decarboxylase (SAMDC), a key enzyme
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FESPB 2010 - XVII Congress of the F
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POST 04 POSTERS • Environmental S
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Author Index Aarts, M.G. S18-002 Ab
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Cattivelli, L. P01-031 Cawly, J. P1
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Gallego, B. P17-037 Gallego, P.P. P
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Kersten, B. P10-017 Keskin, O. P05-
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Miguel, C. S02-001, P01-122 Milhinh
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Ramiro, M. P09-018 Ramon, M P13-002
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Tanimoto, E. P01-045 Tarakanov, I.
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