Book of Abstracts - Geyseco

FESPB 2010 - XVII Congress of the Federation of European Societies of Plant Biology
content between transgenic and untransformed plants grown either
in the presence or the absence of 120 mM NaCl. Growth
in 120 mM NaCl provoked an increase in Na and a decrease in
K content in roots and shoots of all plants analyzed as well as a
decrease in water content in roots, shoots and leaves, although
the decrease was lower in transgenic tomato than in untransformed
plants. An increased expression of the high affinity K uptake
system HAK5 in roots of transgenic plants grown under K limitation
was also detected. Together, the increased expression of
LeNHX2 and HAK5 in transgenic plants is consistent with their
higher K content and indicates the fundamental role of K homeostasis
in the better performance of LeNHX2 overexpressing
plants grown under NaCl stress.
Venema et al 2003. J Biol Chem 278: 22453-9
Rodríguez-Rosales et al 2008. New Phytol 179: 366-377
Work financed by grant BIO2008-01691 from MICINN, Spain
P01-031: MAPMAN VISUALIZATION OF GENE EX-
PRESSION IN DIFFERENT ORGANS OF WINTER BAR-
LEY DURING COLD ACCLIMATION
Janská, A. 1 * - Aprile, A. 2 - Ovesná, J. 1 - Zámecník, J. 1 - Attivelli, L. 3
1
Crop Research Institute
2
University of Salento
3
Agricultural Research Council of Italy
*Corresponding author, e-mail: janska@vurv.cz
MapMan software is a very useful tool for visualizing a big
amount of transcriptional data in metabolic pathways which help
to organize the whole data set to the functional groups. We have
compared the transcriptional profiles in the progress (0, 1, 3, 7,
21d in 3 °C, 1d in -3 °C) of cold acclimation in two distinct organs
(crown and leaf) of winter barley. Freezing tests of both
leaves and crowns were also provided. Using Affymetrix chips
and GeneSpring software 6197 differentially expressed genes
were identified in leaves comparisons and 2370 were significantly
modulated in crowns. The comparisons were visualized
in MapMan software and analysed the up/down regulated genes
involved in the metabolic pathways, especially that involved
in sugar responses. Moreover, the three cluster analyses were
carried out in the GeneSpring software on the list of common
differentially modulated genes (1371 probe sets), on the crown
specific modulated genes (999 probe sets) and on the leaf specific
modulated genes (4827 probe sets). Each cluster was characterized
by a typical course and functional categories determined by
MIPS database. Interesting clusters, functional cathegories and
examples of differentially expressed genes will be presented.
The work was supported by the Grant Agency of Charles University
(project no. 84309), by the National Agency for Agricultural
Research (QH 81287) and by the Czech Ministry of Agriculture
(MZe0002700604).
P01-032: FEATURES OF REACTION OF SEEDLINGS OF
SOFT WHEAT AND ITS WILD RELATIVES ON ACTION
OF ABIOTIC STRESSES
Terletskaya, N.* - Rysbekova, A. - Iskakova, A. -
Khailenko, N. - Polimbetova, F.
Institute of Plant Biology and Biotechnology National, Center
of Biotechnology Ministry of Education and Science Republic of
Kazakhstan
*Corresponding author, e-mail: teni02@mail.ru
Lack of water of soil and air, salt are the most abiotic stresses
in Kazakhstan which create threat of a life of plants, brake their
growth and reduce productivity. Modern selection uses various
approaches for improvement of cultivars of wheat, including
a genofund of world collections and leaning as on methods of
the remote hybridization, and direct carrying over of genes. But
the basic mechanisms of salt tolerance, wheat inherent in wild
relatives are not known practically and their chromosomes are
not conjugation in meiosys with genome of Triticum aestivum
L., technologies transgenesys are almost unsuitable now. Other
approaches of increase of wheat tolerance to stresses are focused
on studying of physiological mechanisms for detection of new
genes of stress-stability of grain cereals. Wild representatives
Triticeae Dum. are growing in a wide series of conditions all over
the world and have the big genetic variations. It is revealed that
among representatives of its, containing even halophytes, there is
a considerable variability in stability to osmotic and salt stress.
Revealing of features of reaction of wheat – T. aestivum L.
– and its wild relatives on abiotic stresses action are caused by
doubtless interest.
As material for researches kinds of wheat served: T. monococum
L. (AuAu), T. pseudomonococcum L. (AuAu), T. sinskaya
Filat. et Kurk. (AbAb), T. polonicum L. (AuAuBB), T. aethiopicum
Jakubz. (AuAuBB), T. dicoccum Shuebl. (AuAuBB), T.
turgidum L. (AuAuBB), T. macha L. (AuAuBB), T. compactum
L. (AuAuBBDD), T. V. rufulum (AuAuBBDD), T. spelta L.
(AuAuBBDD), T. kiharae Dorof. et Migusch. (AtAtGGDD), and
also T. aestivum L. (AuAuBBDD) (grades Caratovskaja-29, Mironovskaja-808,
Leningradka) and Secale сereale L.
Features of reaction on action of abiotic stresses of wheat T. aestivum
L. and its wild relatives were studied. Various reaction of wheat
species to stresses was established, that testifies to necessity of
variety of approaches at selection of genetic material for improvement
of modern wheat cultivars.
P01-033: SHORT-TERM SALT AND OSMOTIC STRESS
IN MAMILLARIA GRACILLIS PFEIFF. TISSUE CULTU-
RE
Balen, B. 1 * - Rogic, T. 1 - Simac, M. 1 - Peharec, P. 1 - Bar-Zvi, D. 2
- Krsnik-Rasol, M. 1
1
University of Zagreb, Faculty of Science, Department of Molecular
Biology
2
Department of Life Sciences, Ben-Gurion University of the Negev
*Corresponding author, e-mail: bbalen@zg.biol.pmf.hr
In vitro propagated M. gracilis plants develop calli without
any exogenous growth regulators. This habituated calli spontaneously
regenerate morphologically normal and hyperhydric
shoots. Since the habituation and hyperhydricity are both part
of a neoplastic progression, cactus cells were transformed with
A. tumefaciens strain B6S3. Tumor line, which was established,
never expressed any morphogenic capacity.
The aim of this study was to investigate the effect of short-term
salt and osmotic stress on the M. gracilis callus and tumor culture.
Tissue explants were grown in a liquid nutrient and exposed
for 15 min and 3 h to growth medium supplemented with 250
mM NaCl or 500 mM mannitol. Following treatments, tissue was
collected and protein extracts were prepared.
They were examined with regard to abundance and phosphorylation
of ASR1 protein by western blotting and Pro Q Diamond
fluorescent dye, respectively. Glycosylation pattern was examined
by Pro Q Emerald fluorescent dye and lectin assay. ASR1
was present in both cactus tissues with three isoforms of approximately
42, 26 and 17 kDa.
No difference in abundance or phosphorylation of ASR1 in callus
and tumor was observed after either 15 min or 3 h treatment with
salt. More intensive phosphorylation signal of ASR1 was noticed
in callus grown on medium with mannitol for 3 h. Analysis
of glycosylation pattern with lectins Con A and GNA indicated
stronger protein glycosylation in callus exposed to mannitol after
15 min and 3 h.
P01-034: VARIABILITY OF THE ARABIDOPSIS THA-
LIANA PHENOTYPE – GENOME AND ENVIRONMENT
Jansen, M.* - Gilmer, F. - Braun, S. - Schurr, U. - Walter, A.
Forschungszentrum Jülich
*Corresponding author, e-mail: m.jansen@fz-juelich.de