Book of Abstracts - Geyseco

FESPB 2010 - XVII Congress of the Federation of European Societies of Plant Biology
of microtubers to arid weight of branches was attained in high
consistencies of BAP (10 mgl -1 ) and Sucrose (8%). The media
having high consistencies of BAP (5 mgl -1 ) and Sucrose (80 gl -1 ),
were of the utmost number of microtubers whereas the maximum
fresh weight of microtubers appeared in media containing BAP
(5 mgl -1 ) and Sucrose (60 gl -1 ). Howsoever, high consistencies
of Sucrose along with high consistencies of BAP reduced the induction
period and also decreased the microtubers formation to
two weeks. Both Sucrose and BAP were of a paramount role on
decrease and increase in microtubers’ fresh weights. An escalation
in Sucrose consistency had a significant impact on rise of dry
weight and microtubers’ biomass. To sum up, in order to select
the most suitable induction media, not only the number and fresh
weight of induced microtubers are to be considered but also other
parameters such as health, dormancy period and the proportion
of dry weight of microtubers to dry weight of branches should be
fully taken into regard.
P05-013: THE EFFICIENCY OF OAT (AVENA SATIVA L.)
HAPLOID PLANT PRODUCTION VIA POLLINATION
BY MAIZE (ZEA MAYS L.)
Stawicka, A.* - Skrzypek, E. - Czyczylo-Mysza, I. – Marcinska, I.
The Franciszek Gorski Institute of Plant Physiology Polish Academy
of Science
*Corresponding author e-mail: stawicka@gmail.com
Oat (Avena sativa L.) is an important cereal cultivated both as
animal fodder and valuable source of nutrients for people. Doubled
haploids (DH) allow enhancing new cultivars breeding by
improving quality of grains and shortening the time of production.
Obtaining DH in cereals is possible mostly by anthers culture
and wide crossing. Oat DH production still remains difficulties
comparing with other cereals.
The aim of the study was to improve the oat haploid embryo
production and ability to developing green plants. Forty four oat
genotypes were used in the experiment. Florets were emasculated
before anthesis and after that they were pollinated with maize
pollen. Next picloram and dicamba were applied on ovaries. Embryos
were isolated 3 to 4.5 weeks after pollination and placed on
TL3 and 190-2 media with maltose. They were grown at 4°C for
0, 1 and 2 days in darkness and then at 21°C in 16h photoperiod.
Ovaries treated with dicamba produced more embryos (4.1/100
florets) compared to picloram (3.5/100 florets). After 3 weeks of
culture 66.7% of embryos treated with dicamba and 57.8% with
picloram germinated. After 6 weeks 24.2% and 27.7% of them
(respectively) developed into
plants. Embryo germination decreased with time of their isolation
(from 92.3% after 3 weeks to 56.9% after 4.5 weeks). Kind
of medium significantly influenced embryo development. After
3 weeks 40.4% embryos germinated on 190-2 and after 6 weeks
21.2% of them produced plants, whereas on TL3 23.6% embryos
germinated and 5.6% produced plants. One day of treatment
with cold increased embryo development into plants (30.6%)
compared to 2 days of cold (15.3%) and 0 days of cold (24.6%).
Chromosome doubling using colchicine and acclimatization until
grain maturation were successful.
P05-014: LITHOSPERMUM CANESCENS (MICHX.)
LEHM. HAIRY ROOTS CULTURE AS A SOURCE OF
RED NAPHTHOQUINONES DEMONSTRATING CYTO-
TOXIC ACTIVITY
Pietrosiuk, A.¹ - Syklowska-Baranek, K.¹ – Kawiak, A.² - Jeziorek,
M.¹ - Lojkowska, E.² - Chinou, I.³
¹Department of Biology and Pharmaceutical Botany, Faculty of
Pharmacy, Medical University of Warsaw
²Intercollegiate Faculty of Biotechnology, Medical University of
Gdansk, Department of Biotechnology, Poland
³University of Athens, School of Pharmacy, Deptartment of Pharmacognosy,
Greece
*Corresponding author e-mail:agnieszka.pietrosiuk@wum.edu.pl
Lithospermum canescens (Boraginaceae) is native to Northern
America. It contains shikonin type pigments:
acetylshikonin (ACS) and isobutyrylshikonin (IBS) [1]. Biological
studies of shikonin derivatives showed a broad spectrum of
their activities [2]. Hairy roots of L.canescens were maintained
in liquid LS medium [3]. To enhance the red pigment production
roots were cultivated for 3 weeks in liquid M9 medium [4].
In this conditions, the total ACS and IBS content (182.01 mg/l)
increased almost 10 fold [5]. Apart ACS and IBS, the detailed
phytochemical analysis of the red coloured fraction revealed the
presence of four alkanines and shikanofurans C and D [6]. The
extracts from transgenic roots were submitted to cytotoxicity assay
against HL-60, HeLa and HaCaT cell lines. They proved to
be the most potent against HL-60 cells (IC 50
= 4 ± 0.3 μg/ml) after
24 hours while for HeLa and HaCaT cells the IC 50
values were 20
± 1.2 μg/ml and 45 ± 2.5 μg/ml, respectively.
1. Pietrosiuk A., Wiedenfeld H. (2005). Pharmaceutical Biology,
43: 189-191.
2. Papageogiou V.P. et al. (2006). Current Organic Chemistry,10:
2123-2142
3. Linsmaier E.F., Skoog F. (1965). Physiologia Plantarum, 18:
100-127.
4. Fujita Y. et al. (1981). Plant Cell Reports, 1: 61-63.
5. Sykłowska-Baranek K. et al. (in press).
6. Graikou K. et al. (in press).
P05-015: CELL SUSPENSION AND CALLUS CULTURES
OF ARNEBIA EUCHROMA (ROYLE) JOHNST. FOR
PRODUCTION
Syklowska-Baranek, K.¹* – Pietrosiuk, A.¹ – Kawiak, A.² - Jeziorek,
M.¹ - Lojkowska, E.² - Chinou, I. 3
¹Medical University of Warsaw, Department of Biology and
Pharmaceutical Botany
²Intercollegiate Faculty of Biotechnologym – Medical University
of Gdansk, Department of Biotechnology, Poland
³University of Athens, School of Pharmacy, Department of Pharmacognosy,
Greece
*Corresponding author e-mail: k.syklowska@yahoo.com
Arnebia euchroma (Boraginaceae) is a perennial herbaceous plant
which grows widely on the mountains between 2100 and 3300 m
altitude in Tianshan, Xinjiang [1]. The roots of A.euchroma are
rich in naphthoquinone compounds, shikonin and its derivatives
which shows several medicinal properties [2]. In comparison to
Lithospermum erythrorhizon, A.euchroma contains much higher
pigment contents and is regarded as a better source of shikoninrelated
compounds [3, 4]. The callus tissue of A euchroma were
maintained on MSA solid medium [5]. The cell suspension culture
was established by transferring callus tissue to liquid MSA
medium. The chemical investigation of deep red pigment fraction
revealed the presence of two major constituents: acetylshikonin,
isobutyrylshikonin [4] and eight alkanins [6]. Combined extracts
prepared from callus and cells suspension were submitted to
cytotoxicity assay against HL-60, HeLa and HaCaT cell lines and
proved to be the most potent against HL-60 cells (IC 50
= 0.75 ±
0.01 μg/ml) after 24 hours while for HeLa and HaCaT cells there
was no activity observed.
1. Jiang B. et al. (2005). In vitro Cellular and Developmental
Biology – Plant, 41: 677-681.
2. Papageogiou V.P. et al. (2006). Current Organic Chemistry,10:
2123-2142
3. Ge F. et al. (2003). Chinese Traditional Herbal Drugs, 34: 7-10.
4. Pietrosiuk A. et al. (1999). Herba Polonica, 45:354-361.
5. Davydenkov V.N. et al. (1991). Him Farm Zh, 1: 53-55.
6. Damianakos H. et al. (in press).
P05-016: THE STUDY OF POTATO’S MICROTUBERIZA-
TION RESPONSES (SOLANUM TUBERSUM L.) IN HIS-
TOLOGICAL TISSUE CULTURE CONDITIONS TO THE
VARIOUS LEVELS OF BENZYL AMINO POURINE