Book of Abstracts - Geyseco

P - Posters
correlated to potential induction of defence related physiological
changes occurring during tuber storage. Therefore, disease
symptoms and defence related metabolic changes are analysed in
seed tubersdiffering by physiological age after inoculation with
Pectobacterium atrosepticum. Disease symptoms are evaluated
by measuring the weight of soft rot. Activity of PAL is determined
spectrophotometrically and total phenolic compounds are
quantified by using a method described by Folin and Ciocalteu.
Results and conclusions will be presented during the conference.
Kumar GXM, Knowles NR. 2003. Wound-induced superoxide
production and pal activity decline with potato tuber age and
wound healing ability. Physiologia Plantarum 117(1)1: 08-117.
determination of antioxidant compounds (glutation, ascorbate)
were conducted at different post-infection times. The differences
in virulence between the viral strains are reflecting in most of the
analyzed parameters.
Work supported by grant MCINN AGL2008-00214.
P17-076: ESSENTIAL OIL CONSTITUENTS OF PERO-
VSKIA ATRIPLICIFOLIA BENTH. - ANTIMICROBIAL
EFFECTS
Zamfirache, M.¹ – Burzo, I.² - Gostin, I.N.¹ - Stefan, M.¹ - Badea
M L.² - Olteanu, Z.¹ - Truta, E.³
¹The Alexandru Ioan Cuza University of Iasi
²USAMV Bucharest
³ICB Iasi
P
The structure and micromorphology of the glandular trichomes,
chemical composition and antimicrobial effects of the secreted
essential oil of Perovskia atriplicifolia were studied. The volatile
oils extraction was conducted using a Clevenger hydrodistillation
system. The component separation was performed by gas
chromatography using a 6890 Agilant GC-MS. The volatile compound
identification was made using the NIST spectral bank and
Kovats indexes. Antimicrobial effects were tested using a standard
difusimetric method. SEM analysis revealed that leaves
have numerous glandular trichomes of three morphological distinct
types, all a unicellular foot and stalk: capitate hairs with
bi-cellular head, peltate hairs with tetra-cellular head and peltate
hairs multicellular (10-celullar) head. Qualitative and quantitative
GC-MS analyses of the essential oils revealed limonene (18
%), γ – terpinene (16 %), β - caryophyllene (13 %), α - caryophyllene
(12 %) and cymene (11%) as the main constituents. The
investigated volatile oils have antimicrobial effects on Staphylococcus
aureus (depending on concentration) and a mild influence
on the growth and development of Escherichia coli.
P17-077: OXIDATIVE STRESS AND ANTIOXIDANT
RESPONSE DURING THE INFECTION OF NICOTIANA
BENTHAMIANA WITH PMMOV
Hakmaoui, J.¹ - García-Fontana, B.¹ - Jiménez, A.² - Camejo,
D.² - Sevilla, F.² - Barón, M.¹
¹Estación Experimental del Zaidín. CSIC
²Centro Edafología y Biología Aplicada del Segura. CSIC
In some compatible virus–host plant interactions, an oxidative
stress is produced manifested as an increase in different
oxidative stress parameters (lipid peroxidation, protein oxidation
and electrolyte leakage), accumulation of H2O2, as well as an
imbalance in the antioxidative systems. Some authors have proposed
that disturbances on the photosynthetic electron transport
during viral pathogenesis induce an increased ROS generation.
We have previously showed that the oxygen-evolving complex
of photosystem II was the chloroplastidic target of the Pepper
mild motle tobamovirus (PMMoV). In addition, PMMoV infection
induced the down regulation of several chloroplastidic
proteins involved in both the photosynthetic electron transport
and the Benson–Calvin cycle (Pineda et al. 2010. Photosynth.
Res. 103:31). The present study was carried out to link the
viral-induced effect on the chloroplast to ROS generation during
viral pathogenesis. N. benthamiana plants were infected with the
Italian and Spanish strains of the PMMoV. Analysis of the oxidative
stress biomarkers (lipid peroxidation, protein carbonylation,
H2O2 production), evaluation of ROS-scavenging capacity by
different enzymes (CAT, APX, SOD, MDHAR, GR) as well as