Book of Abstracts - Geyseco

P - Posters
lics have a central role in the habituation of maize cell cultures
to dichlobenil.
P01-090: ADAPTIVE PECULIARITIES OF DECIDUOUS
MAGNOLIAS DURING THE INTRODUCTION IN UKRA-
INE
Vovk, M. - Palagecha, R.
T. Shevchenko Kyiv National University, Fomin Botanical Garden
*Corresponding author, e-mail: slawawowk@yahoo.com
For the first time are established reasons of different hardiness of
annual shoots in 11 species of Magnoli a L. introduced in Ukraine.
The anatomy-morphological peculiarities of shoot structure
connected with different winter resistance are presence of thick
cuticle (10-28 mkm), multilayer periderm, subepidermal sclerenchymatous
elements; formation of secretory containers which
accumulates secondary metabolism matters in bark; intensive
lignification of cell membranes; different density of lenticels
arrangement. In experiments with artificial freezing of shoots
(under -25, -30 and -35°C) was established decreasing of tissue
hardiness in sequence from bark to medulla. High water storage
capacity and more contents of bound water in annual shoots are
presupposition for formation of winter resistance. In the shoots
of winter resistant species was the bound water twice as more
then in species with low winter resistance. Increase of flavonoides
quantity in bark during vegetation and its decrease in winter
testifies about their participation in processes of wintering. Acclimatization
in natural conditions (temp. from +3,2 to -10°C) and
in laboratory (temp. -30 and -35°C) accompanied by changes of
total flavonoides contents (decreasing in middle on 34%).
In annual shoots tissue established differences in localization of
lipid compounds and dynamics of starch and lignin and has been
determined their specific role in winter resistance of different
species of magnolias. The adaptive changes of lipidic index of
resistance proved in increasing of galactolipids content in bark
during preparation of plants to the wintering and their decreasing
in winter. Shown also decreasing of sulfolipids content in bark of
magnolia shoots owing to decreasing of temperature.
P01-091: EFFECTS OF TREATMENT WITH ADOR ON
OXIDATIVE ACTIVITY IN AXENIC SUNFLOWER
ROOTS
Garrido, I. 1 - García-Sánchez, M. 2 - Garrido-Romera, I. 2 -
Ocampo, J.A. 2 - Espinosa, F. 1 *
1
Universidad Extremadura
2
Estación Experimental Zaidín, CSIC
*Corresponding author, e-mail: espinosa@unex.es
The aim of this work was to analyze the effect of aqueous-extract
of dry olive-mill residue (ADOR), uninoculated and inoculated
with saprobic fungi, on sunflower growth. The O 2
-
formation and
exogenous NADH oxidation activities, apoplastic peroxidases
(DMAB-MBTH POX and CA-POX), total antioxidant capacity,
total phenols, flavonoids, and phenylpropanoids, and membrane
lipid peroxidation were determined in intact sunflower roots
germinated and grown for 36 h without (control) or with 50%
ADOR, and with or without prior incubation with saprobic fungi
(Pychoporus cinnabarinus, Coriolopsis rigida, Trametes versicolor,
and Penicillium crysogenum). Treatment of the control
roots with ADOR for 10 min induced an increase in both O 2
-
formation
and DMAB-MBTH EC-POX activity, possibly due to
oxidative shock in response to the stress caused by the ADOR.
The roots germinated in uninoculated ADOR, however, presented
marked decreases in oxidative activity, total antioxidant capacity,
and phenol and phenylpropanoid contents. These activities
were partially recovered in the roots germinated in the inoculated
ADOR, although without reaching the normal values. The
most effective fungal were P. cinnabarinus and P. crysogenum.
Treatment with ADOR was also observed to increase membrane
lipid peroxidation.
The effects induced by ADOR may be caused by alterations in
the plasmamembrane, which could then affect membrane-linked
enzyme systems.
P01-092: ROLE OF HEAT DISSIPATION MECHANISMS
IN PHYSCOMITRELLA PATENS ACCLIMATION TO DI-
FFERENT LIGHT CONDITIONS
Gerotto, C. 1 * - Alboresi, A. 2 - Bassi, R. 2 - Giacometti, G. 1 - Morosinotto,
T .1
1
Dipartimento di Biologia, Università di Padova
2
Dipartimento di Biotecnologie, Università di Verona
*Corresponding author, e-mail: caterina.gerotto@unipd.it
Sun light provides energy supporting life of photosynthetic organisms
but also leads to the formation of reactive oxygen species
when in excess.Thus, plants and algae evolved several photoprotective
processes to survive in a variable environment. The fastest
one, called Non Photochemical Quenching (NPQ), consists
in the dissipation of excess energy as heat, which is triggered
by the generation of a pH gradient across thylakoid membranes.
Green algae and plants are all able to induce NPQ, but its activation
depends on two different proteins: LHCSR and PsbS,
respectively. The moss Physcomitrella patens is the only known
organism where both PsbS and LHCSR are present and active
in NPQ.
Here we show that acclimation to different light conditions has
a strong influence on NPQ in P.patens: when acclimated to high
light WT mosses showed an enhanced NPQ which is correlated
to the increased expression of both PsbS and LHCSR. Overexpression
of PsbS and LHCSR in transgenic plants confirmed that
the level of both proteins controls NPQ amplitude. Conversely,
KO mutants depleted in PsbS and LHCSR showed reduced capacity
of NPQ which, interestingly, was accompanied by an enhanced
susceptibility to long-term light stress. Our results thus point
to the relevance of NPQ in the long-term acclimation besides
being a short-term response: despite it is a fast activated mechanism,
NPQ modulation is also fundamental for acclimation to
prolonged light stress.
P01-093: THE STUDY OF PHYSIOLOGICAL RESPON-
SES OF MUSA ACUMINATA VAR. MAS TO INTERAC-
TION OF SALINITY AND CADMIUM
Farzami Sepehr, M. 1 * - Harikrishna J.A. 2 - Norzulaani, K. 2
1
Dep of Biology, Faculty of Agriculture, Islamic Azad University
Saveh Branch
2
Genetics and Molecular Biology, Institute of Biological Sciences
,Faculty of Science, University of Malaya ,Kuala Lumpur,
Malaysia
*Corresponding author, e-mail: mfsepehr48@yahoo.com
Soil salinity affects plant growth and development due to harmful
ion effects and water stress caused by reduced osmotic potential
in the soil solution. Furthermore, Cd is a pollutant that has
been emitted into the environment for decades. Major anthropogenic
sources are Cd-containing phosphate fertilizers, sewage
sludge and industrial emissions. Plants undergo one or more
stress during their life cycle. The effects of 0,25,50 μM Cd 2+
(Cd(NO 3
) 2
.4H 2
O) and 0,50,75,100,125,150 mM NaCl on growth
, the content of some ions and proline contents in Banana (Musa
acuminata var. Mas) were investigated in present study. With increasing
concentrations of Cd 2+ or NaCl alone in culture media,
growth parameters, Chlorophylls and proline contents decreased.
Combination treatment with salinity and cadmium decreased the
negative effects observed following the two stress alone. Plants
exhibiting growth retardation, none cadmium accumulation in
response to one mild stress factor (75,100,125 mM NaCl).the
exposure of plants to cadmium caused a partial reversal of effect
of salinity. Root and shoot growth, ion accumulation, sensitivity
index and other physiological responses were improved at moderate
concentrations of two stress factors imposed jointly.
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