Book of Abstracts - Geyseco

P - Posters
Skoczowski, A.*
Polish Academy of Sciences, The Franciszek Gorski Institute of
Plant Physiology
*Corresponding author, e-mail: skoczowski@ifr-pan.krakow.pl
Broccoli leaves were injured by the ozone dose applied (70 ppb,
6 hours daily) mainly under WBL (WBL - white supplemented
by blue radiation). Studies were carried out on leaf fragments
from the outer (OP) and the inner parts (IP). The spectrophotometric
analysis did not reveal differences in the Chl content.
The variations in reflectance (R) in the visible region (400-700
nm) were not significant with respect to tissue age, ozone fumigation
and growth conditions (WBL or WL - white radiation).
Only in plants grown under WBL an increase in green R in ozone-treated
leaves was observed, indicting a decrease in the chlorophyll
content. Measurements of the optical properties of leaves
provide more precise information about changes in the content
of chlorophyll than spectrophotometry. Leaves under WBL
showed significant differences in R within 800-1100 nm, which
depended on the physiological age of the tissue. For physiologically
older tissues, values of R were lower than those for physiologically
younger tissues. However, under WL, a decrease in R
within the 800-1100 nm range depended only on ozone fumigation.
Response of broccoli leaves to spectral composition of irradiation
and ozone stress was much higher for irradiation transmission
(T) than for R. Ozone fumigation leaves grown in WBL
increased T in OP and IP leaf fragments, both in the 500-700 and
750-1100 nm ranges. For plants growing in WL no influence of
ozone fumigation on the T of irradiation within the visible range
was observed. However, fumigation with ozone resulted in a
significant decrease in the T within the infrared range (750-1100
nm). The results indicate the major role of irradiation spectral
composition in plant response to ozone stress.
P01-099: POST-TRANSCRIPTIONAL REGULATION OF
STRESS-INDUCED MRNA DECAY BY 3’UTR OF GENES
IN RICE
Kim, Y. 1 * - Park, S.H. 1 - Chung P.J. 2 - Felipe Redillas, M.C. 1 -
Kim, J.K. 1
1
Myongji University
2
Rockefeller University, NY, USA
*Corresponding author, e-mail: younshic@gmail.com
Many environmental stimuli including water potential, temperature
extremes, and high salinity, regulate gene expression at
transcriptional and post-transcriptional levels. We are taking a
genomics-based approach to unravel the regulation in response
to such environmental stresses in rice. Expression profiling with
the 135K Rice Whole Genome Microarray revealed that transcripts
of a group of genes involved in light and dark reactions
are decayed much earlier than the others under stress conditions.
Changes in polysomal mRNA are similar with those of total
RNA. The stress-induced mRNA decay was discovered a posttranscriptional
event by using RNA pol II chromatin immunoprecipitation
assay. To delineate functional determinant(s), we
chose two representative genes, RbcS1 and Cab1, and dissected
them into several components. Transgenic rice plants expressing
different combinations of the components were analyzed under
stress conditions using the real-time qPCR method, demonstrating
that 3’UTR is the major mRNA sequence determinant that
mediates such stress-induced mRNA decay.
P01-100: EPR INVESTIGATION OF LONG LIVED RADI-
CALS IN WHEAT SEEDS
Filek, M. 1 * - Labanowska, M. 2 - Bidzinska, E. 2 - Biesaga-Koscielniak,
J. 1
1
The F. Gorski Institute of Plant Physiology, Polish Academy of
Sciences
2
Faculty of Chemistry, Jagiellonian University
*Corresponding author, e-mail: mariafilek@gmail.com
Electron Paramagnetic Resonance (EPR) is one of the very useful
method to study of various types of radicals generated in
biological materials during life processes. Among these radicals
long lived species seems to be responsible for protection of cells
against different stresses. The aim of this work was investigation
of long lived radicals in seeds of two wheat genotypes, Polish
and Finnish, which exhibit various stress response. EPR spectra
of both genotypes reveal the similar signals characteristic for sugar
radicals. In the Finnish wheat, the intensity of these signals
was about two times lower than in Polish one. Cutting of seeds
stimulates the appearing of the additional signal but only in the
EPR spectrum of the Polish seeds. This phenomenon was accompanied
with the disappearance of the intensive signal of iron species
visible in not cutting seeds. The latter was not observed in
the seed of Finnish genotype.
This work was supported by COST FA0605, Nr: DPN/N110/
COST/2009
P01-101: DROUGHT STRESS TOLERANCE AND THE
ANTIOXIDANT ENZYME SYSTEM IN SPRING WHEAT
Biesaga-Koscielniak, J. - Filek, M. - Dziurka, M. - Koscielniak,
J. - Pitera, R. -
The F. Gorski Institute of Plant Physiology, Polish Academy of
Sciences
*Corresponding author, e-mail: j.koscielniak@ifr-pan.krakow.pl
Plants experience drought stress either when the water supply to
roots becomes difficult or when the transpiration rate becomes
very high. Although the general influence of drought on plant
growth are fairly well known, the primary effects of water deficit
at the biochemical and the molecular levels are not well understood.
Drought induces oxidative stress in plants by producing reactive
oxygen species (ROS). Oxidative damage in the plant tissue
is alleviated by a concerted action of both enzymatic and non-enzymatic
antioxidant metabolisms. The relationship between the
antioxidant enzyme system and drought stress tolerance during
leaf rolling in the leaf of sprig wheat was studied. 20 cultivars
of spring wheat were used in the study. Plants were cultivated in
growth room in pots filled at a 16-h photoperiod, at irradiance of
450 μmol×s -1 ×m -2 . Some plants were well watered (control) whereas
the other plants were subjected to drought stress. Analyses
were carried out after a 3-week period of drought. Changes in
non-enzymatic and enzymatic antioxidant responses were used
to assess the effects of drought stress. The reaction of all investigated
genotypes to drought stress was typical i.e. an increase of
the antioxidative enzymes level was observed. At the same time
the levels of tocopherols, carotenoids and phenols increase also
when comparing to the non-stressed plants.
This work was supported by COST FA0604 Tritigen Nr: 192/
C0ST/2008/0 and COST FA0605, Nr: DPN/N110/COST/2009
P01-102: QUANTITATIVE DETERMINATION OF RE-
DOX-ACTIVE POLYPHENOLICS FROM NITROGEN
STRESSED RED BEETROOT (BETA VULGARIS L.)
PLANTS BY ELECTRON PARAMAGNETIC RESONAN-
CE SPECTROSCOPY.
Papasavvas, A 1 - Stathi, P. 1 - Salahas, G 1 - Hela, D. 2 - Deligiannakis,
Y. 1
1
Lab. of Physical Chemistry, Dept. of Environmental and Natural
Resources Management, University of Ioannina
2
School of Natural Resources and Enterprise Management, University
of Ioannina
Plant polyphenolics are the most widely distributed secondary
plant products (30-45%) of plant organic matter, and have been
shown to accumulate frequently as a reaction to environmental
stress, including nitrogen starvation. The total Stress-Induced
Polyphenolics (SIP) can be determined by liquid chromatography
(HPLC), or spectrophotometric assays. However the antoxidant
activity of the SIPs is the redox-active fraction, which is
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