Book of Abstracts - Geyseco

FESPB 2010 - XVII Congress of the Federation of European Societies of Plant Biology
ChF could help to determine response of host plants to inoculated
fungi. Rooted microshoots were inoculated in 2006 year. Spring
2007 they were transplanted into the field. Each year (until 2009),
ChF was measured using MINI-PAM fluorometer. Morphological
response and later on, yielding were also measured.
On the base of ChF measurements and morphological evaluation
we concluded:
• Fungi isolated from Vaccinium spp. growing in polish
ecosystems were able to form mycorrhizal symbiosis with
V. macrocarpon, genus native to North America.
• Among multiple ChF parameters, ETR and qP were the
most suitable to assess host plant response to symbiotic
fungi.
• At beginning, fungi played a role of partial parasites, decreasing
photosynthetic activity of host plants.
• Mycorrhizal symbiosis established only in the third year of
plants growth in the field. Values ETR and qP were higher
for mycorrhized plants than non-mycorrhized. Acknowledgement
The experiments were conducted within the framework of a project
No DPN/N83/COST/2009
P01-015: EFFECTS OF AQUEOUS EXTRACT FROM DRY
OLIVE RESIDUE (ADOR) ON THE PHYSIOLOGY OF
TOMATO PLANTS: OXIDATIVE STRESS AND CHANGE
IN THE ANTIOXIDANTS SYSTEMS
García Sánchez M* - Aranda, E. - Reina, R. - Ocampo, J.A. -
Garcia-Romera, I.
Consejo Superior de Investigaciones Científicas
*Corresponding author, e-mail: mercedes.garcia@eez.csic.es
The aim of this work was to analyse the effect of aqueous extracts
of dry olive mill residue (ADOR), non treated and treated with
saprobe fungi, in the physiology of Solanum lycopersicum plants
in order to recycle dry olive residue for fertilizers. In addition,
we tried to determine if the ADOR use enhanced lipid peroxidation
(MDA) and H 2
O 2
levels and induced changes on antioxidant
systems: Superoxido Dismutase (SOD), Ascorbate Peroxidase
(APX) and Glutatione Reductase (GR). ADOR, obtained by
orbital-shaking of dry olive residue, was used as the growth medium
of the following fungi: Coriolopsis rigida and Penicillium
chrysogenum. ADOR treated and not treated with saprobe fungi
at 5% dose were applied to tomato plants during 4, 10 and 30
days.
We observed that treated and non treated ADOR with saprobe
fungi induces higher MDA and H 2
O 2
levels on roots and leaves
of tomato plants after 4 and 10 days of exposure. However, after
30 days of exposure, MDA and H 2
O 2
levels only increased in
response to non treated ADOR. On roots, after 4 and 10 days
of exposure, we observed an increase in APX and GR activities
whereas, on leaves, the increase was found on SOD, APX and
GR activities. After 30 days of exposure we observed an increase
in APX and SOD on roots and leaves of tomato plants.
We concluded that ADOR induces an oxidative stress through
the generation of ROS and activates the antioxidant system of tomato
plants. However, we observed that the treatment of ADOR
with saprobe fungi palliated this effect mainly in a long-term exposition
due to the bioremediation effect by these fungi.
P01-016: USE OF REDOX-SENSING ROGFP1 FOR MO-
NITORING REDOX CHANGES IN THE CYTOSOL AND
MITOCHONDRIA IN WATER STRESSED ARABIDOPSIS
THALIANA PLANTS
Brossa Gonzalez, R. 1 * - Jubany-Marí, T. 1 - Alegre Batlle, L. 1 -
Jiang, K. 2 - Feldman, L. J. 2
1
Universitat de Barcelona
2
University of California, Berkeley
*Corresponding author, e-mail: rbrossa@ub.edu
Water deficit can induce redox changes in plant cell compartments
and therefore maintenance of redox homeostasis is one
of the plant’s mechanisms to cope with stress. Knowledge
of where redox changes occur, and their kinetics and magnitude,
is crucial to understanding the responses of plants to environmental
stress. The expression of Reduction-Oxidation Green
Fluorescent Proteins (roGFP1) in plants has become a useful
tool as it provides in vivo direct measurement of redox state, as
well as dynamic measurements over the time and in different
intracellular locations.Real-time measurements of redox state in
A.thaliana plants under a drought stress treatment were reported.
For this effort seeds of A.thaliana ecotype Col-0 were transformed
with roGFP1 which was expressed either in the cytosol
(c-roGFP1) or in the mitochondria (m-roGFP1). Five-week-old
plants of the two transformed lines were subjected to two irrigation
treatments: Well watered (WW), and Water stressed (WS).
The time course of leaf water relations, content of reduced and
oxidized forms of ascorbate and glutathione, and cytosolic and
mitochondrial leaf redox state (measured using c-roGFP1 and
m-roGFP1) were monitored.In this work changes in the redox
state were reported in both the cytosol and mitochondria of leaf
cells during the time course of drought stress. As the plant became
more dehydrated the redox state of the leaf cytosol became
more oxidized compared to its initial redox state. However,
the mitochondria of WS plants decreased their redox potential
(becoming less oxidized) with increasing periods of WS. This
suggests that under WS the cellular compartments respond differently
(presumably adaptively) to WS, exhibiting an enhanced
protection against oxidative stress in mitochondria.
P01-017: REDOX CHANGES DURING COLD ACCLI-
MATION IN WHEAT GENOTYPES WITH DIFFERENT
FREEZING TOLERANCE
Kocsy, G* - Soltész, A. - Tímár, I. - Kellos, T. - Vashegyi, I. - Szalai,
G. - Galiba, G.
Agricultural Research Institute of the Hungarian Academy of
Sciences
*Corresponding author, e-mail: kocsyg@mail.mgki.hu
The aim of the present study was to find out whether redox changes
induced by cold hardening are related to freezing tolerance
and vernalization requirement in a specific genetic system containing
chromosome 5A substitution lines. The amounts of H2O2
and ascorbate, the ratio of ascorbate to dehydroascorbate exhibited
a rapid transient increase in the crown, followed by a gradual
increase during the subsequent two weeks. The amount of glutathione
(GSH) and its ratio to glutathione disulphide (GSSG) first
decreased, while later increased. The H2O2 (measured in crown
extract and visualized by fluorescence staining in the shoot apex),
ascorbate and GSH concentrations, the ascorbate/dehydroascorbate
and GSH/GSSG ratios and the half-cell reduction potential
of the GSH/GSSG couple were higher in the freezing-tolerant
genotype and the corresponding substitution line than in the sensitive
genotypes during the second half of the hardening period.
In contrast to H2O2 and the non-enzymatic antioxidants, the lipid
peroxide concentration and the activity of the four antioxidant
enzymes studied, exhibited a transient increase during the first
week, with no significant difference between the genotypes. While
the observed redox changes correlated with freezing tolerance
in the crowns, they were independent from the vegetative/generative
transition state monitored by the apex morphology and the
vernalization gene expression. This work was supported by the
Hungarian Scientific Research Fund and the National Office for
Research and Technology (NKTH-OTKA K67906 and K68894).
P01-018: OSBADH1 IS POSSIBLY INVOLVED IN THE
OXIDATION OF ACETALDEHYDE IN RICE PEROXISO-
MES
Mitsuya, S.* - Yokota, Y. - Fujiwara, T. - Takabe, T.
Graduate School of Bioagricultural Sciences, Nagoya University
*Corresponding author, e-mail: mitsuya@agr.nagoya-u.ac.jp