Book of Abstracts - Geyseco

More documents

Recommendations

Info

P - Posters Gould, P.¹ - Foreman, J.² - Domijan, M.³ - Piñas Fernández, A.² - Costa, M.³ - MacGregor, D.4 - Zielinski, T. ² - Steward, K.² - Bird, S.4 - Steel, G.² - Williams, M.² - White, M.¹ - Graham, I. 4 - Penfield, S. 4 - Finkenstadt, B.³ - Rand, D.³ - Millar, A.² - Hall, A¹ - Halliday, K.² ¹University of Liverpool ²University of Edinburgh ³University of Warwick 4University of York Changes in ambient temperature can have dramatic consequences for plant development and physiology. Temperature changes alter the reaction rates of individual biochemical processes; therefore, a biological network must balance the effects of these alterations in each of its components to retain signalling integrity over a temperature range. ROBuST seeks to understand the principles that underlie SENSITIVITY and ROBUSTNESS of a biological network. Our study network comprises the interconnected network of light, cold acclimation and the circadian pathways. Our work has identified a new and prominent function for cry1, cry2, and phyA as regulators of temperature buffering of the circadian clock. Deficiencies in cry1 and cry2 resulted in failure of the clock transcriptional feedback loop under warm temperatures, and phyA deficiency under cold. This work has led to i) the development of temperature compensated plant clock model, and ii) evidence the co-evolution of light and temperature signal transduction. P03-014: METABOLITE ANALYSIS OF AN ABA-DEFI- CIENT MUTANT OF SWEET ORANGE DURING RIPE- NING AND POSTHARVEST STORAGE Romero, P. 1 * - Lafuente, M.T. 1 - Reyes-de-Corcuera, J.I. 2 - Alférez, F. 1 - Zacarías, L. 1 - Rodrigo, M.J. 1 - Burns, J. K. 2 1 Instituto de Agroquímica y Tecnología de Alimentos (IATA- CSIC) 2 Citrus Research and Education Center, University of Florida *Corresponding author e-mail: ciepro@iata.csic.es ‘Pinalate’ is a yellow-colored spontaneous mutant fruit from the ‘Navelate’ orange (Citrus sinensis L. Osbeck). Carotenoid biosynthesis is blocked in this mutant, resulting in abscisic acid (ABA) deficiency and accumulation of non-coloured carotenoids. Fruit of this mutant display altered ripening and increased susceptibility to peel pitting during storage at non-chilling temperatures (12ºC). Flavedo (colored part of the peel) samples from both cultivars were collected at several ripening stages and stored at various durations at 12 ºC to characterize their metabolic profile by GC-MS. Compounds were identified and grouped into amino acid, sugar, polyalcohol and organic acid families. All identified sugars increased during ripening in both cultivars; however, sucrose content fluctuated and reached higher values in mutant mature fruit. Other compounds such as serine and ribitol decreased with ripening while indole-3-acetic and mannoonic acids sharply increased at the last ripening stage in both varieties. The results also showed that at 12 ºC most sugars similarly decreased in both cultivars. Among organic acids, only hexadecanoate showed a different pattern, reaching higher levels in ’Pinalate’ after prolonged storage. Taken together, the results revealed that only sucrose and hexadecanoate of studied compounds showed differences between ‘Navelate’ and ‘Pinalate’ fruits, suggesting that these changes may be associated with the altered behavior of ‘Pinalate’ mutant. P03-015: A REVERSE GENETICS APPROACH TO THE ANALYSIS OF LEAF DEVELOPMENT Muñoz-Viana, R. - Rubio-Díaz, S. - Pérez-Pérez, J.M. - Wilson- Sánchez, D. - Ponce, M.R. - Micol, J.L. División de Genética and Instituto de Bioingeniería, Universidad MiguelHernández, Campus de Elche, Alicante, Spain Because of their photosynthetic activity, leaves are the ultimate source of most of the oxygen that we breathe and of the food that we eat. Yet the processes by which these organs grow are poorly understood. Previous forward genetics studies yielded a large number of mutations affecting Arabidopsis leaf development, shape or size. However, none of these earlier attempts reached genome saturation. The group of Prof. J.R. Ecker at the Salk Institute is obtaining a large collection of gene-indexed homozygous T-DNA insertion mutants that will cover 25,000 genes of the Arabidopsis genome. To identify novel genes required for leaf growth regulation, we have begun a reverse genetics screening using the 14,000 T-DNA insertion lines available in batches from the ABRC, which correspond to 10,800 different Arabidopsis genes. These lines are grown in vitro and those exhibiting aberrant leaf phenotypes are documented and kept for further studies. In order to saturate the Arabidopsis genome with viable and fertile leaf mutations, we plan to screen the entire Salk homozygous T- DNA insertion collection for visible leaf phenotypes. P03-016: A COLLECTION OF AMIRNAS TARGETING GROUPS OF TRANSCRIPTION FACTOR-ENCODING PARALOGS Ponce, M. - Jover-Gil, S. - Micol, J.L. División de Genética and Instituto de Bioingeniería, Universidad Miguel Hernández, Campus de Elche (Alicante), Spain Our understanding of the function of individual Arabidopsis genes is obscured by the existence of gene families that include redundant members. In fact, there is an expanding list of single null mutants not exhibiting a mutant phenotype. In addition, examples are known of double and even triple combinations of non-allelic, loss-of-function mutations affecting paralog genes that cause no visible phenotypes. The masking effects of redundancy in gene families can be overcome with new technologies based on gene silencing using artificial microRNAs (amiRNAs). We are obtaining transgenic Arabidopsis lines expressing amiRNAs designed to repress groups of paralog genes encoding transcription factors. These amiRNAs were designed to target groups of two or more transcription factor-encoding genes that are arranged in tandem in the Arabidopsis genome. Following the design principles that can be found at http://wmd.weigelworld.org, we already designed 197 amiRNAs, 164 of which have already been transferred to Arabidopsis plants. Three well known transcription factor-encoding genes with easily visible loss-of-function phenotypes were chosen as controls: GL1, AG and PAP1. In most, but not all cases the transgenic plants obtained exhibited the phenotype expected from downregulation of the target gene. P03-017: METABOLIC PROFILES CAN ASSIST TO DIS- CRIMINATE CAULIFLOWER GROWN UNDER DIFFE- RENT FARMING PRACTICES Annunziata, M. – Massaro, G. – Iannuzzi, F. – Nacca, F. – Carillo, P. - Fuggi A. Dipartimento di Scienze della Vita, Seconda Università di Napoli, Caserta Keywords: cauliflower, Brassica oleracea L. Subsp botrytis, carbohydrates, aminoacids, glucosinolates The conventional cauliflower culture needs high inputs of fertilizers and pesticides, that can cause soil pollution and degradation. On the other hand, the demand of better foods and the use of more sustainable agricultural practices is increasing. In this view the aim of this work was to verify if metabolite profiling can discriminate among farming practices and assist in tracing the produce. Cauliflower (Brassica oleracea subsp. botrytis cv Atalaya) plants were grown under traditional farming system (TFS) and conservative (low tillage) farming system (CFS). At the harvest the corymb heads were divided in curd (immature flowers) and stem, frozen and homogenised in liquid nitrogen. Aliquots were used to determine the content of protein, carbohydrates, ascorbate and glutathione, inorganic and organic acids, free amino acids and glucosinolates. Five individual plants were used as replicates. P
FESPB 2010 - XVII Congress of the Federation of European Societies of Plant Biology Multivariate analysis of the results related to such metabolites allowed to discriminate between curd and stem of the cauliflower heads along the first principal component accounting for about 60% and between the farming practices along the second principal component for about 25%. Financial support was obtained by “Seconda Università degli Studi di Napoli”, “Ministero dell’Università” and “Ricerca Scientifica e tecnologica” of Italy (PRIN 2006077008_005; 2008S9T3KK_003).
Page 4:
FESPB 2010 - XVII Congress of the F
Page 7 and 8:
PLENAR SESSION LECTUR
Page 9 and 10:
Page 11 and 12:
Page 13 and 14:
PARALL SESSION LECTUR
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30: FESPB 2010 - XVII Congress of the F
Page 39 and 40: POST 04 POSTERS • Environmental S
Page 79: FESPB 2010 - XVII Congress of the F
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 206 and 207:
Author Index Aarts, M.G. S18-002 Ab
Page 208 and 209:
Cattivelli, L. P01-031 Cawly, J. P1
Page 210 and 211:
Gallego, B. P17-037 Gallego, P.P. P
Page 212 and 213:
Kersten, B. P10-017 Keskin, O. P05-
Page 214 and 215:
Miguel, C. S02-001, P01-122 Milhinh
Page 216 and 217:
Ramiro, M. P09-018 Ramon, M P13-002
Page 218 and 219:
Tanimoto, E. P01-045 Tarakanov, I.
show all

Book of Abstracts - Geyseco

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?