Book of Abstracts - Geyseco

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P - Posters in late phase of senescence caused the increase of aminoacids content, which was the result of cessation of phloem transport. Phenological forms of European beech differed in initial data of senescence of the leaves as well as different periods of senescence phases. They also had different ability of protein compound remobilization. Early form of beech tree remobilized 74% of total proteins, while intermediate 73% and late form remobilized 68% of all proteins. The average level of remobilization of phenological forms of beech tree was 80% of soluble proteins and 64% of insoluble proteins. P09-017: GENETIC DIVERSITY OF POLISH ISOLATES OF HYMENOSCYPHUS, THE TELEOMORPH OF CHA- LARA FRAXINEA, THE CAUSAL AGENT OF ASH DIE- BACK Zarek, M.* - Kraj, W. University of Agriculture in Krakow *Corresponding author, e-mail: emzarek@gmail.com Random Amplified Microsatellites (RAMS) markers were used to investigate the genetic structure and variation of Hymenoscyphus, the teleomorph of Chalara fraxinea, which causes dieback of Fraxinus excelsior in Europe. Ninety five isolates, obtained from ascospores, which represent six populations from lowland and upland parts of Poland were analyzed. 72 (89%) out of 81 bands generated with four RAMS primers were polymorphic. The lowland and upland groups of isolates were obtained by using PCA analysis. Percentage of polymorphic loci was higher for upland (87.7) than for lowland (81.5) isolates. The genotypic diversity inferred from Shannon’s index was higher for upland (0.422±0.028) than for lowland (0.390±0.028) isolates. Dice a similarity coefficient, which was the second measure of intrapopulation variation, also showed higher genetic differentiation of upland (0.74±0.002) than lowland (0.78±0.003) isolates. AMOVA partitioned the total variation into 77% intrapopulation, 19% between-population and 4% between upland and lowland isolates. This analysis and Nei genetic distance between pairs of populations showed that differentiation among populations was high and depended on population elevations. It appeared that the main factor which influences the genetic variation level is climatic conditions. As a result of greater differentiation of climatic conditions in upland region, the genetic variability of fungus was greater, which allows better toleration of varied external conditions. P09-018: GENETICAL, MOLECULAR AND ECOLOGI- CAL ANALYSES OF FLOWERING VERNALIZATION RESPONSES IN ARABIDOPSIS THALIANA Méndez-Vigo, B. - Ramiro, M. - Pozas, J. - Sánchez, E. - Martínez-Zapater, J.M. - Picó, X. - Alonso-Blanco, C. Centro Nacional de Biotecnología (CSIC) *Corresponding author, e-mail: bmendez@cnb.csic.es Wild genotypes of Arabidopsis thaliana collected from different natural populations show substantial variation for the acceleration of flowering initiation induced by long exposure to low temperatures, i.e. for their vernalization response (Alonso-Blanco et al., 2009). To determine the amount of quantitative variation existing for this response we have analysed flowering time in a collection of 183 genotypes from different populations of the Iberian Peninsula (Picó et al., 2008), grown with 0, 1, 2 or 3 months at 4 °C. This analysis shows that 17% of Iberian accessions have an obligate vernalization requirement, while several genotypes without such requirement present a stronger response than laboratory strains. We have selected Ll-0 and Ped-0 accessions with different extreme vernalization responses to obtain two new populations of recombinant inbred lines (RILs) derived from crosses with the reference strain Landsberg erecta (Ler). To determine the genetic bases of natural variation for vernalization response we have measured the flowering time of these two RIL populations grown under different vernalization periods and we have carried out QTL mapping analyses of those data. On the other hand, to find out part of the molecular bases of this variation we have sequenced the FRIGIDA gene of the 183 accessions and we have carried out association analyses between FRI polymorphism and the flowering phenotypes of this collection. Finally, aiming to identify environmental factors that might drive FRI genetic variation we have compared FRI polymorphisms with geographic and climatic factors of the natural populations of origin. References Alonso-Blanco et al. 2009 Plant Cell 21:1877-1896 Picó et al. 2008 Genetics 180:1009-1021. P09-019: ASSOCIATION MAPPING: EXPLORING ALLE- LIC RESPONSES FOR COMPLEX TRAITS Ishihara, H.* - Sulpice, R. - Pyl, E-T. - Stitt, M. Max-Planck-Institute of Molecular Plant Physiology *Corresponding author, e-mail: ishihara@mpimp-golm.mpg.de A. thaliana is a suitable model for QTL mapping of a wide range of simple or even complex traits. However, this approach is limited by the genetic and phenotypic diversity of the two parents comprising mapping populations. Association mapping is overcome these limits as it makes use of the variation existing in a large number of natural populations. However, full genome association mapping requires a very good coverage of the genome in a large number of accessions for the detection of associations to complex traits. SNP data generated in the frame of the 1001 genome project and the Arabidopsis thaliana “HapMap” project allow such coverage, but not definite identification of the responsible polymorphisms for an observed association. In the laboratory, we developed a strategy to identify candidate genes involved in the regulation of biomass and then make association mapping using their full sequence (Sulpice et al. 2009). Briefly, by determining other traits (metabolites, enzymes and transcripts) and analysing their connections together and with biomass, we could point the potential importance of two candidate genes. The two genes were then fully sequenced by Sanger method in >90 accessions and several associations could indeed be identified with biomass but also to some metabolic traits. However, to validate such approach, other genes should be tested. One of the major issues in our previous study appeared to be the necessity of getting high quality sequences for a large number of accessions. After selection of 31 additional genes potentially involved in the regulation of biomass, we are now sequencing them by 454 barcode sequencing. Based on the associations obtained, the validity of this strategy will be discussed. P09-020: SCREENING FOR NITROGEN USE EFFICIEN- CY (NUE) IN HUNGARIAN POTATO CULTIVARS Hoffmann, B.* - Hoffmann, S. - Polgár, Z. University of Pannonia *Corresponding author, e-mail: hoff-b@georgikon.hu Crop production is highly dependent on the supply of exogenous nitrogen (N) fertilizers. With increased fertilizer application rate the risks of N loss increase rapidly. The remaining N is lost as either surface runoff; leached nitrate in groundwater or by volatilization to the atmosphere; microbial denitrification, all of which pose environmental concerns. Although nitrate losses may be reduced through improved N fertilizer management practices, nitrate losses are still excessive under commercial production regions. Another approach may be to reduce nitrate loss by developing potato cultivars which utilize N more efficiently. Nitrogen use efficiency (NUE) is defined as dry matter production per unit N supply. Because of the critical role of N rate in achieving economic and environmental objectives, screening for genotypes with better NUE may reduce production costs and contamination of the environment by maximizing fertilizer utilization. P
FESPB 2010 - XVII Congress of the Federation of European Societies of Plant Biology In this study variation in nitrogen use efficiency (NUE: dry matter production per unit crop N supply) characteristics of commercial potato cultivars of Hungarian origin were evaluated. Cultivars were grown with (50-, or 100 kg N ha–1) or without application of N fertilizer. The experiment was set up as a split block design with fertilizer rates as main plots and the cultivars as sub-plots.
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FESPB 2010 - XVII Congress of the F
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POST 04 POSTERS • Environmental S
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Author Index Aarts, M.G. S18-002 Ab
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Cattivelli, L. P01-031 Cawly, J. P1
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Gallego, B. P17-037 Gallego, P.P. P
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Kersten, B. P10-017 Keskin, O. P05-
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Miguel, C. S02-001, P01-122 Milhinh
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Ramiro, M. P09-018 Ramon, M P13-002
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Tanimoto, E. P01-045 Tarakanov, I.
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