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126 IV.Resultados y discusión. Capítulo II
is being chewed and swallowed are between 10 and 100 s -1 (McClements,
2004).
n
y
K
(1)
n1
K
(2)
2.3.4 Colour parameters
Colour coordinates were measured from the reflection spectrum in a
spectrocolorimeter CM-3600 d (MINOLTA Co; Osaka, Japan). A 20 mm
depth cell was used. CIE L * a * b coordinates were obtained using illuminant
D65/10º observer. Lightness (L * ), chrome (C * ab) and hue (h * ab) of the
different samples as well as colour difference (E) (equations 3 to 5) with
respect to the non-fermented sample were obtained.
*
*2 *2
C ab a b
(3)
b
*
*
h ab arctg
(4)
*
a
* 2 * 2 *
L
a
b
2
E
(5)
2.3.5 Confocal laser scanning microscopy (CLSM)
A Nikon confocal microscope C1 unit, which was fitted on a Nikon
Eclipse E800 microscope (Nikon, Tokyo, Japan), was used. An Ar laser line
(488 nm) was employed as light source to excite fluorescent dyes
Rhodamine B and Nile Red. Rhodamine B (Fluka, Sigma-Aldrich, Missouri,