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UNIVERSITAT POLITÈCNICA DE VALÈNCIA Desarrollo ... - RiuNet

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IV. Resultados y discusión. Capítulo II 211<br />

2.4 Statistical Analysis<br />

The results were analysed by means of a multifactor analysis of<br />

variance using Statgraphics ® Centurion XVI. Multiple comparisons were<br />

performed through 95% LSD intervals.<br />

3. RESULTS AND DISCUSSION<br />

3.1 Effect of growth factors on fermentation process<br />

Table 1 shows the experimental responses for the fermentation time<br />

(Y 1 ) and GG counts (Y 2 ) obtained for each formulation of the CCD. All the<br />

formulations were suitable as a means of developing a probiotic hazelnut<br />

fermented milk, since the probiotic survival was over 7 log cfu/mL in every<br />

case, which is the minimum recommended probiotic amount in order to<br />

ensure health effects in consumers (Gomes and Malcata, 1999; Stanton et<br />

al., 2003; Van Niel et al., 2002). Moreover, the duration of the fermentation<br />

process was also appropriate, since standard cow milk fermentations are<br />

generally developed in 3-4 h (Alais, 1998). Other authors observed longer<br />

fermentation times (≈ 6 h) in dairy yoghurt processing when GG and<br />

standard yoghurt bacteria were used as starters (Hekmat and Reid, 2006).

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