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within an area extending several tens <strong>of</strong> metres beyond the discharge point.<br />

Cool water discharged at the surface layer is carried by the northward<br />

current but at a lower depth.<br />

2.5.2. Enrichment experiment<br />

As part <strong>of</strong> the <strong>environmental</strong> impact research for manganese nodule<br />

mining, experiments were carried out to determine the impact <strong>of</strong> the mining<br />

effluent on phytoplankton. These experiments was conducted to determine<br />

the effects <strong>of</strong> introducing deep-sea water, which contains high nutrient<br />

concentrations, on dominant pico- <strong>and</strong> nanoplankton.<br />

Results <strong>of</strong> the experiments showed that the addition <strong>of</strong> deep water<br />

could cause an increase in chlorophyll a <strong>and</strong> changes in the composition <strong>of</strong><br />

these communities. In experiments where the concentration <strong>of</strong> bottom<br />

water was low, cell-number maxima were attained in a shorter period. In<br />

contrast, in experiments with high concentrations <strong>of</strong> bottom water, it took<br />

more days to reach the maximum <strong>and</strong> the values were higher than those in<br />

other experiments with lower concentration (figure 7). However, at higher<br />

concentrations (50% surface seawater <strong>and</strong> 50% bottom water),<br />

micr<strong>of</strong>lagellates increased with decreasing nitrate <strong>and</strong> nitrite<br />

concentrations, <strong>and</strong> Bacillariophyceae showed an increase at lower<br />

concentrations. In all the experiments the daily growth rate <strong>of</strong><br />

bacillariophyceans increased with increasing initial concentrations, though<br />

cyanobacteria <strong>and</strong> micr<strong>of</strong>lagellates showed no significant correlation with<br />

bottom-water concentrations.<br />

These results suggest that the concentrations <strong>of</strong> nitrate <strong>and</strong> nitrite<br />

can influence succession <strong>of</strong> phytoplankton communities.<br />

3. Benthic Environmental Study<br />

The field study <strong>of</strong> the benthic environment, which included both<br />

baseline <strong>and</strong> impact assessment research (the latter labelled JET: Japan<br />

Deep-Sea Impact Experiment), was performed in the western part <strong>of</strong> the<br />

exclusive Japanese claim area in the Central Pacific Ocean (figure 8). The<br />

purpose <strong>of</strong> JET was to evaluate the effect <strong>of</strong> artificial rapid sediment<br />

deposition (=disturbance), which was supposed to occur during commercial<br />

mining. This experiment consisted <strong>of</strong> four field studies, as follows:<br />

JET 1: the field study conducted before the disturbance;<br />

JET 2: the field study conducted immediately after the disturbance;<br />

INTERNATIONAL SEABED AUTHORITY 197

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