standardization of environmental data and information - International ...

?? Fixation of zooplankton with formalin
?? Identification and enumeration under a microscope
?? Converting the count numbers to individuals/m 3 according to the
filtered volume of seawater measured by a flowmeter attached to
the mouth of the net
3.2.2. Biomass
?? Freezing the sample and transferring to the laboratory
?? Drying samples in an oven at 60º for 24 hours
?? Measuring dry weight on a microbalance (Mettler Co.)
?? Measuring carbon and nitrogen content using AA (atomic
absorption) (EA 1110, Carlo Ebra, Inc.)
?? Calculating C/N ratio
3.2.3. Grazing experiments (effects of suspended sediment)
?? Sampling seawater from the surface, and filling a 2-l polycarbonate
experimental bottle (Nalgene)
?? Collecting copepods to be used in the experiments by towing a net,
and separating them from the ambient water
?? Putting five adult copepods in the experimental and control bottles
(n=4)
?? Adding sediment of 0.002, 0.02, 0.2 and 2.0 g to each treatment
?? Incubating the experimental bottles for 12 hours
?? Measuring chlorophyll a concentration, calculating grazing rates and
investigating the effect of suspended sediments on the grazing
rates of copepods
INTERNATIONAL SEABED AUTHORITY 243