standardization of environmental data and information - International ...

3.3. Zooplankton (protozoan)
3.3.1. Species composition and biomass
?? Sampling seawater in 1000-ml and 60-ml quantities for protozoans
and heterotrophic flagellates, using 10-l Niskin bottles
?? Fixing protozoans with Lugol's solution in a final concentration of
1.0% and flagellates with glutaraldehyde in a final concentration of
0.3%
?? Enumerating protozoans under an inverted microscope (Olympus IX-
70), and classifying flagellates into two groups, heterotrophic and
autotrophic flagellates, using dyes such as DAPI and Primulin
?? Investigating the distribution patterns of mixotrophic ciliates
3.3.2. Grazing rates
?? Sampling 12-l seawater bottles and filtering protozoans through a
200-µm mesh screen
?? Using a 2.7-l polycarbonate (PC) bottle for incubation
?? Diluting the 200-µm filtered seawater with 0.45-µm filtered
seawater by 0, 30, 55 and 80%, and adding nutrients such as
nitrate and iron to stimulate phytoplankton growth
?? Measuring chlorophyll a concentration at t=t0 by filtering 500-µm
seawater
?? Incubating experimental bottles for 24 hours and measuring
chlorophyll a
4. Clculation and estimation of nodule Abundance and
metal tonnage
4.1. Nodule abundance at stations
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