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standardization of environmental data and information - International ...

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a. Meteorological variables: sea state, wind speed <strong>and</strong> direction,<br />

cloud cover<br />

b. Conductivity-temperature-depth (CTD) pr<strong>of</strong>iles in the top 1000<br />

metres: conductivity <strong>and</strong> salinity, temperature, water depth, light<br />

level, chlorophyll a, dissolved oxygen<br />

c. Water samples to calibrate the CTD <strong>and</strong> to determine nutrient<br />

levels to be taken at the surface, within the mixed layer, at the<br />

base <strong>of</strong> the mixed layer, <strong>and</strong> within the subsurface chlorophyllmaximum<br />

<strong>and</strong> oxygen-minimum zones<br />

d. Routine measurements from water samples:<br />

i. Nutrients (silicate, nitrate, phosphate)<br />

ii. Dissolved oxygen<br />

iii. Chlorophyll<br />

iv. Salinity<br />

e. An oblique tow for zooplankton from the sea surface to 200 m<br />

depth with a 200-micron mesh net. Use <strong>of</strong> a st<strong>and</strong>ard (60-<br />

centimetre-diameter mouth opening) bongo net with flow meter<br />

is recommended. Displacement volume will be measured at a<br />

minimum.<br />

CTD <strong>and</strong> water-sample analysis will follow the protocols <strong>of</strong> the Joint<br />

Global Ocean Flux Study (JGOFS) 1 . Zooplankton sampling will follow the<br />

protocols <strong>of</strong> the Zooplankton Methodology Manual <strong>of</strong> the <strong>International</strong><br />

Council for the Exploration <strong>of</strong> the Sea 2 .<br />

2. Optional oceanographic sampling<br />

Further useful <strong>information</strong> can be obtained from:<br />

1. Epifluorescence-microscope counts <strong>of</strong> bacterial cell<br />

abundance <strong>and</strong> biomass, <strong>and</strong> <strong>of</strong> phytoplankton to assess<br />

species composition<br />

2. Inverted-microscope counts <strong>of</strong> settled microzooplankton<br />

samples<br />

3. Analysis <strong>of</strong> particulate organic carbon (POC) <strong>and</strong> nitrogen<br />

(PON) from water samples<br />

524<br />

INTERNATIONAL SEABED AUTHORITY

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