POSTER ABSTRACTS - ISAKOS

the anterolateral tibial cortex. Excellent reduction
and stable fixation was achieved, as well as
improved circular tension around the entire
medial meniscus. Shortly thereafter we also
encountered a 50-year old male who sustained an
acute injury to the knee involveing an ACL
avulsion as well as the meniscal ossicle avulsion.
Using the same technique, the meniscal ossicle
was successfully fixed with less difficulty. The ACL
avulsion fracture was also fixed at the same time.
These cases support the avulsion theory of the
posterior root of the meniscus as the etiology of
the meniscal ossicle. Furthermore, we describe a
novel technique for repair of an acute meniscus
ossicle. It is reasonable to consider this repair
technique in subacute and chronic cases to
restore biomechanical integrity of the knee joint.
E-poster #712
Osteochondritis Dissecans (OCD) of the Lateral
Femoral Condyle
Mitsuo Ochi, Hiroshima, JAPAN,
Nobuo Adachi, Hiroshima, Hiroshima JAPAN
Yoshio Sumen, Onomichi, Hiroshima JAPAN
Kenzo Kawasaki, Izumo, Shimane JAPAN
Masataka Deie, JAPAN, Presenter
Orthopaedic surgery Hiroshima University,
Hiroshima, JAPAN
We examined the relationship between
osteochondritis dissecans (OCDs) of the lateral
femoral condyle and lateral menisci. From 1993
to 2002, we experienced 43 OCDs of the lateral
femoral condyle. OCD locations were graded by
the Cahill and Berg 17 classification. From the
anterior-posterior view, 26 OCDs were located in
zone 4 and 17 OCDs were located in zone 5. The
lateral menisci were complete discoid in 19 knees,
incomplete discoid in 15, normal in 4, and postmenisectomy
in 5. Ten of the 19 complete discoid
menisci were damaged. The type of a lateral
meniscus was significantly graded a complete
discoid meniscus without tears when the OCD was
located in zone 4, was significantly an incomplete
discoid meniscus when the OCD was located in
zone 5. We determined that OCDs of the lateral
femoral condyle were affected by the type and
state of the lateral meniscus.
E-poster #713
Study on Human Chondrocyte Culture Viability
for Autologous Transplantation in Clinical
Application
Moises Cohen, Sao Paulo, BRAZIL, Presenter
Christiane Lombello, Sao Paulo, BRAZIL
G. M. Reis Jr., Campinas, SP BRAZIL
ALBERT EINSTEIN ISRALE HOSPITAL/UNIFESP,
Sao Paulo, BRAZIL
Objective: The limited regenerative capacity of the
cartilage tissue makes the treatment of chondral
lesions difficult. The currently available
techniques to treat lesions of the articular
cartilage may relieve symptoms, but do not
regenerate the injured tissue. Autologous
chondrocyte transplantation uses techniques of
cell biology and cell culture to regenerate the
hyaline cartilage. Methods: In this study, the
collection and chondrocyte culture phases were
analyzed, aiming at autologous transplantation.
Ultrastructural analyses of biopsies from the
hyaline cartilage were performed 0, 6, 24 and 48
hours after collection. Even after 48 hours, the
tissue was well preserved. Eleven cell culture
assays were performed to evaluate isolation,
viability, morphology, proliferation and absence of
contaminants. Results: Conditions of the cell
culture techniques used allowed chondrocyte
proliferation. Rates on cell viability were
maintained above the acceptable patterns (above
90% ). Control of cell culture laboratory
conditions showed absence of contaminants,
assuring safety of the process. The cells obtained
presented the typical morphology of chondrocytes
cultivated as monolayers. Conclusion: The results
indicate viability of the technique of chondrocyte
culture for clinical application in autologous
transplantation.
KEYWORDS: Cell culture;
Chondrocytes/transplantation; Transplantation,
autologous/methods; CARTILAGE
E-poster #714
The Pathogenesis of Osteochondritis Dissecans
in the Lateral Femoral Condyle Associated with
Lateral Discoid Meniscus Injury
Takashi Terashima, Hokkaido, JAPAN, Presenter
Yasumitsu Ohkoshi, Hakodate, Hokkaido, JAPAN
Kazuki Yamamoto, Hakodate, Hokkaido, JAPAN
Wataru Ebata, Hakodate, Hokkaido, JAPAN
Shinya Nagasaki, Hakodate, Hokkaido, JAPAN
Jun Nishiike, kushiro, Hokkaido, JAPAN
Tomoyuki Hashimoto Hakodate, Hokkaido,
JAPAN
Shigeru Yamane Hakodate, Hokkaido, JAPAN
Hakodate Central General Hospital, Hokkaido,
JAPAN