POSTER ABSTRACTS - ISAKOS

(Hyaluronic Acid), PRGF (Plasma Rich in Growth
Factors), CO (Chondroitine Sulphate), PCB
(Placebo), HIPRGF (Hyaluronic Acid + Plasma
Rich in Growth Factors), COPRGF (Chondroitine
Sulphate + Plasma Rich in Growth Factors) and
each group was divided into 3 sub-groups
according to survival time: 35 days, 70 days and
140 days post-treatment.
Surgery:
Sedation: dissociative agent + ?2 agoniste sedant
+ opiate
General blood exam (auricular artery)
Knee x-rays
Limb shave and lavage
Induction: Isofluorane inhalation.
Surgical set-up
Medial knee arthrotomy: a chondral lesion of 2
mm reaching subchondral bone was created in
support area of the medial femoral condyle
Joint injection according to group
Post-op period:
Medical treatment: analgesic, antiinflammatory
and antibiotic.
Daily dressing until complete healing
During survival time, the animals had complete
freedom of movement and limb functional
exercises were assisted by staff.
All animals in joint injection groups received 4
injections with 1-week intervals. The same
sedation protocol was done each time.
The chondroprotectors animals´group received
the substance via esophagic catheter. The
treatment started 15 days pre-surgery and
continued during all the survival period.
When survival period finished, all animals were
sacrificed following:
Sedation: dissociative agent + ?2 agoniste sedant
+ opiate
Functional exam + synovial citology
Sacrifice: commercial euthanasic
Collection of samples
Results
The data statistically analysed to determine the
treatment’s effectiveness in each experimental
group were:
1.- Active and passive function
2.- Blood exam
3.- Synovial citology
4.- Specific X-rays
5.- Macroscopic vision post-arthrotomy
6.- Chondral histological exam
Acknowledges
The authors thanks to: Angel Duran, Diego López,
Victor Trilla, Xavier Totusaus, Ivan Serra & Carme
Soler
E-poster #725
Repair of Large Osteochondral Defect After
Septic Arthritis Using Mesenchymal Stem Cell
Nobuo Adachi, Hiroshima, JAPAN, Presenter
Mitsuo Ochi, Hiroshima, Hiroshima JAPAN
Masataka Deie, Hiroshima, Hiroshima JAPAN
Yohei Ito, Hiroshima, Minami-ku JAPAN
Hiroshima University, Hirosihma, JAPAN
In this case report, we present a patient with
severe osteochondral defect after septic arthritis,
successfully treated by the transplantation of
cultured mesenchymal stem cells (MSC) and
interconnected porous calcium hydroxyapatite
ceramics (IP-CHA) hybrid material.
In July 2001, a 21-year-old male construction
worker sustained an open dislocation fracture of
his right knee in a traffic accident. Osteosynthesis
of open medial femoral condyle fracture was
performed in a local hospital. Pus discharge from
his right knee joint appeared 13 days after the
operation, and Methicillin-resistant
staphylococcus aureus grew in a culture of the pus
discharge. After several operations, including
curettage of the lesion and continuous irrigation
of the joint, the patient presented in April 2002
with severe right knee pain and limited range of
motion. The flexion angle of the right knee joint
was severely limited to 40 degrees. He
complained of severe right knee pain especially
while walking and his daily activity was severely
restricted due to pain and motion disturbance of
the joint.
In November, 2003, after diagnostic arthroscopy,
we aspirated bone marrow cells from his left tibia
under local anesthesia, and MSC were harvested
and expanded in a monolayer culture system.
Culturing for 24 days produced 1.15x106 MSC. IP-
CHA had been made to order according to the
osteochondral defect size measured by 3-
dimensional computed tomography. The cultured
MSC and IP-CHA hybrid material were
transplanted to the osteochondral defect of his
right medial femoral condyle. His right knee pain
and click disappeared soon after the operation.
The flexion angle of the knee joint was restored to
120 degrees, enabling him to resume his daily
activities. The second-look arthroscopy 1 year
after the operation revealed that the
osteochondral defect was repaired with smooth