POSTER ABSTRACTS - ISAKOS

carried out a perspective study,parallel between
autologous AGF and staminal cells application in
orthopedy and the ex-vivo valuation in vitro of
platen activation on the patient's osteoblast in 12
cases (5 hip prosthesis mobilisation,5 knee
prosthesis mobilisation,2 knee prosthesis). The
outcome of all cases has been good. The study
has proved that:the PDGF-AA,isoform of pDGF,an
important mitogenous among AGF,increases
more remarkably after adding platen gel to the
osteoblasts than in check cultures which are
lacking in platenet gel. Such increase might mean
the platen activation on the ostoblast and might
play a causal role in the process.
E-poster #166
Cell Viability of Menisci Frozen at Three
Different Temperatures: Experimental Study in
Rabbits
Leandro Reckers, Sao Paulo, BRAZIL, Presenter
Djalma Fagundes, Sao Paulo, BRAZIL
Jose Luiz Pozo Raymundo, Pelotas, BRAZIL
Marcia Bento Moreira, Sao Paulo, BRAZIL
Vanessa Carla Paiva, Sao Paulo, BRAZIL
University of Sao Paulo, Sao Paulo, BRAZIL
Background and Objectives: The successful of
meniscus transplantation was in dependence of
different procedures of storage and the cell
viability. Menisci to be transplanted may be
stored in different ways: frozen, fresh, freeze-dried
and cryopreserved. The purpose was evaluating
menisci morphology, by recording cell death time
and collagen organization, after freezing at three
different temperatures.
Methods: Menisci medial(n=90) and lateral(n=90)
of NEW ZEALAND rabbits(n=45) was aseptic
removed and frozen at 7.3oC, 24oC and 73oC. Four
menisci were thawed every two days for
histological analysis until completing 30 freezing
days. Menisci hematoxilin-eosin stained was
examined by fluorescent microscopic method. A
qualitative score of collagen fibrin disruption was
compared with the standard normal meniscus.
The cell viability was evaluated by morphometric
count of pyknosis index.
Results: The start of appearance of disruption
collagen lawyers was at the 2th (-7.3oC), 4th (-30
oC) and 4th day (-73 oC). The progressive
disorganization was worst at 20th to 30th day with
appearance of a white and amorph substance in
80% of samples. The pyknosis started at same
time of collagen disorganization and at 28th day
there was cell death in virtually all meniscus
extension. Menisci cellularity has changed
according to freezing time.
Conclusion: Our findings have shown that
meniscus freezing leads to cell death in different
days according to temperature. This is vital for
determining meniscus storage time for future
transplantation.
E-poster #167
A Surgical Technique for Autologous Medial
Femoral Condyle Transplantation in Rabbits
Leandro Reckers, Sao Paulo, BRAZIL, Presenter
Djalma Fagundes, Sao Paulo, BRAZIL
Antonio Queiroz, Sao Paulo, BRAZIL
Vanessa Carla Paiva, Sao Paulo, BRAZIL
Marcia Bento Moreira, Sao Paulo, BRAZIL
University of Sao Paulo, Sao Paulo, BRAZIL
Background and Objectives: The focal articular
cartilage injury with loss of substance is still a
surgical challenge. Current treatment is a simple
resection of fragments with future arthritis and
functional restriction. The purpose was to
evaluated a surgical procedure of transplantation
and fixation of the medial femoral condyle in an
experimental animal model.
Methods: NEW ZEALAND rabbits (n=12) was
submitted to surgical procedure in three stages: 1-
medial condyle fragment (10x5x3mm) was
resected in the posterior direction - posterior
cruciate ligament, which coincides with anterior
osteotomy border, was used as reference; 2- bone
fragment was removed and placed in saline
solution for five minutes; 3 bone fragment was
reimplanted in the same animal using 1.0mm
Kirschner’s thread. Euthanasia occurs in 4th week;
the knee was dissected and observed the fixation
of the bone fragment. Samples of bone fragment
and distal femur was prepared for usual
histological procedures and hematoxilin-eosin
stained.
Results: Medial femoral condyle was firmly
attached to the distal femur in 83% of cases (10
knees). Two bone fragments were poorly attached
with movements at manipulation of surgical site.
Intense periosteal reaction was one of histological
aspects of osteo regeneration. Trabecular bone
was present in healing line between bone
fragment and distal femur. Normal mature bone
tissue was observed with hematogenic narrow.
Conclusion: Kirschner’s thread technique was
feasibility for medial femoral condyle fixation in
rabbits. So, we are starting a pilot-study with
homologous medial femoral condyle