December 2012 Number 1 - Utah Native Plant Society

A. lentiginosus var. ursinus
(published type locality)
Calochortiana December 2012 Number 1
Table 1, continued
Taxon Type Map
Code
Herbarium
Voucher
L,IL M GH, NY Palmer s.n. 1877
A. lentiginosus var. ursinus M 1 OSC, UNLV Alexander 2120 [A]
A. lentiginosus var. ursinus
(Mokiak Pass elements mounted with the
type by Gray)
M 2 GH Palmer s.n. 1877
A. lentiginosus var. vitreus H,I U POM, NY Maguire & Blood 4413
A. lentiginosus var. wilsonii v R OSC, UNLV Alexander 2367 [A]
A. lentiginosus var. wilsonii v R 2 OSC, UNLV Alexander 2334 [D]
A. lentiginosus var. wilsonii (type locality) H,I R 3 ND Wilson s.n. from May 1893
A. lentiginosus var. wilsonii
(population intermediate with var. bryantii,
var. mokiacensis, or var. ursinus)
A. lentiginosus var. wilsonii
(population putatively intermediate to var.
palans)
P CAS Eastwood 5748
P 2 NY Demaree 43807
A. lentiginosus var. yuccanus H C POM Jones 3886
cross section (podsc), pod shape in longitudinal section
(podsl), pod pedicel orientation (podpo), leaf abaxial
pubescence (leafab), keel length (keell), calyx teeth
shape (calyxs), and pod orientation on raceme. The second
axis of the PCoA explained 11.7% of the total variation.
The largest correlation to the second axis was
from leaf adaxial pubescence (leafad). Moderate correlations
were obtained for pod stipe length (pods), calyx
teeth shape (calyxs), and wing color (wingc). A scatterplot
of the first two coordinates of the PCoA is shown in
Figure 4.
In PAUP*, an heuristic search of 100 random addition
sequences with TBR branch swapping was started
with the data set of 21 morphological characters from 30
specimens of the Palantia and related members of A.
lentiginosus. All 21 characters were parsimony informative.
Sixteen most parsimonious trees of length 110
were recovered (HI = 0.5636; RI = 0.6416; CI = 0.4364;
RC= 0.2800). Figures 5-8 are the strict consensus of
trees of length 110 with major characters state changes
mapped on the clades. The clades in this analysis have
low support. A bootstrap analysis of 10,000 replicates
resulted in only five clades having 70% or higher support
(pseudiodanthus & iodanthus, clade A, 73%;
vitreus 4413 to yuccanus 3886, clade B, 73%; yuccanus
3886 & ambiguus 2325, clade C, 87%; wilsonii 2367A
& 2334D clade, 75%; maricopae 1621A & 1621C,
clade E, 95%). Only banner color (Figure 5), pod persistence
(excluding the reversal to a deciduous pod in A.
lentiginosus var. wilsonii, Eastwood 5748; Figure 6),
pod raceme orientation (Figure 7) and degree of pod
incurve (not shown) had a high consistency with little or
no character state reversals.
A dendrogram (Figure 9) of a Euclidean similarity
matrix obtained from a cluster analysis showed nearly
the same topology as the tree obtained from the parsimony
analysis.
DISCUSSION
Outgroup selection in this study proved to be problematic.
Barneby (1964) proposed that a plant similar to
the small flowered Astragalus lentiginosus var. salinus
(a taxon with bladdery inflated pods) was the ancestor to
the members of the A. lentiginosus complex and that
this complex was closely related to Section Inflati A.
Gray, a large species complex with unilocular, bladdery
inflated pods. Nuclear inter-transcribed spacer (ITS)
DNA sequence data have shown that A. lentiginosus has
an identical sequence to that of A. purshii Douglas ex
Hook. (and an almost identical sequence to that of A.
utahensis (Torr.) Torr. & A. Gray) of Section Argophylli
A. Gray (a section composed primarily of taxa with
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