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112 KKU Science Journal Volume 39 Number 1 Research Nanakorn, S. Honark, N., Ungpansattawong, S., Chaisiwamongkol, W., Maneesriwongul, A. and Suwanwerakamtorn, R. (2009). Relationship between fingerprint pattern and multiple intelligence: phase 1. Research report. Khon Kaen: Khon Kaen University. (in Thai) Nora, J. J. and Fraser, F. C. (1989). Medical genetics: principles and practice. 3 rd edition. Philadelphia: Lea Febiger. Okajima, M. (1975). Development of dermal ridge in the fetus. Journal of Medical Genetics. 12: 243-250. Penrose, L. S. (1969). Effects of additive genes at many loci compared with those of a set of alleles at one locus in parent-children and sib correlations. Annals of Human Genetics. 33: 15-21. Penrose, L. S. and Ohara, P. T. (1973). The development of the epidermal ridges. Journal of Medical Genetics. 10: 201-208. Rajangam, S. Thomas, L. M. and Janakiram, S. (1995). Dermatoglyphics in Downûs syndrome. Journal of Indian Medical Association. 93: 10-30. Rosa, A., Gutierrez, B., Guerra, A., Arias, B. and Fananas, L. (2001). Dermatoglyphics and abnormal palmar flexion creases as markers of early prenatal stress in children with idiopathic intellectual disability. Journal of Intellectual Disability Research. 45: 416-423. Schaumann, B. A. and Alter, M. (1976). Dermatoglyphics in medical disorders. New York: NY Springer-Verlag. Sintuwong, K., Klomjit, C., Bunterm, T., Chaijareon, S. and Mungsing, W. (2002). Multiple intelligence of Khon Kaen University students. Research report. Khon Kaen: Klungnanavitaya. (in Thai) Smith, M. K. (2008). Howard Gardner and multiple intelligences: the encyclopedia of informal education. (Cited 2 November, 2008). Available from: http://www.infed.org/ thinkers/gardner.htm SPSS Statistics Rel. 17.0 (2009). SPSS SN: 5068054, Chicago: SPSS Inc. USA. ❏❏❏❏❏
«.«‘∑¬. ¡¢. 39(1) 113-119 (2554) KKU Sci. J.39(1) 113-119 (2011) Effect of Chitosan on Growth and Development of Phalaenopsis cornucervi (Breda) Blume & Rchb.f. Somporn Prasertsongskun 1* Witool Chaipakdee 1 ∫∑§—¥¬àÕ ‡µ√’¬¡‰§‚µ´“π®“°·°πÀ¡÷° ‚¥¬¥—¥·ª≈ß®“°«‘∏’¢Õß Alimunair ·≈– Zainuddin (1992) ‰¥âª√‘¡“≥ ‰§‚µ´“π√âÕ¬≈– 20 (w/w) ‡¡◊ËÕπ”‚æ√‚∑§Õ√å¡¢Õß°≈â«¬‰¡â‡Õ◊ÈÕß‡¢“°«“ßÕàÕπ ¡“‡æ“–‡≈’È¬ß„πÕ“À“√‡À≈« Ÿµ√ VW (Vacin and Went, 1949) ∑’Ë‡µ‘¡‰§‚µ´“π∑’Ë‰¥â®“°·°πÀ¡÷° §«“¡‡¢â¡¢âπ 5 10 15 20 ·≈– 25 ¡‘≈≈‘°√—¡/ ≈‘µ√ ‡ªìπ‡«≈“ 4 —ª¥“Àå æ∫«à“‰§‚µ´“π∑ÿ°√–¥—∫§«“¡‡¢â¡¢âπ°√–µÿâπ°“√‡æ‘Ë¡ª√‘¡“≥¢Õß‚æ√‚∑§Õ√å¡ ‡¡◊ËÕ‡ª√’¬∫ ‡∑’¬∫°—∫ÿ¥§«∫§ÿ¡∑’Ë‰¡à‡µ‘¡‰§‚µ´“π ‡¡◊ËÕπ”‚æ√‚∑§Õ√å¡¡“‡æ“–‡≈’È¬ß„πÕ“À“√·¢Áß æ∫«à“‰§‚µ´“π°√–µÿâπ°“√ ‡®√‘≠‡µ‘∫‚µ¢Õß‚æ√‚∑§Õ√å¡‰¥âÕ¬à“ß¡’π—¬ ”§—≠ ‚¥¬‡©æ“–‰§‚µ´“π§«“¡‡¢â¡¢âπ 15 ¡‘≈≈‘°√—¡/≈‘µ√ „Àâ®”π«π „∫ (4.3 „∫/µâπ) ®”π«π√“° (2.5 √“°/µâπ) ·≈–®”π«πÀπàÕ (1.5 ÀπàÕ/µâπ) ‡©≈’Ë¬ Ÿß∑’Ë ÿ¥ Õ¬à“ß‰√°Áµ“¡‡¡◊ËÕ„Àâ ‰§‚µ´“π§«“¡‡¢â¡¢âπ Ÿß¢÷Èπ ®–¬—∫¬—Èß°“√‡®√‘≠‡µ‘∫‚µ¢Õß‚æ√‚∑§Õ√å¡ µâπ°≈â“∑’Ë‡®√‘≠‡µ‘∫‚µ‡¡◊ËÕπ”¬â“¬ÕÕ°ª≈Ÿ°„π «— ¥ÿª≈Ÿ°∑’Ë‡ªìπ∂à“π·≈–√¥¥â«¬‰§‚µ´“π§«“¡‡¢â¡¢âπµà“ßÊ ‡ªìπ‡«≈“ 4 —ª¥“Àå æ∫«à“µâπ°≈â“¡’’«‘µ√Õ¥¡“°°«à“ √âÕ¬≈– 50 Abstract The chitosan was prepared from cuttlebone by the modified method of Alimunair and Zainuddin (1992) with the yield of 20% (w/w). Protocorms of Phalaenopsis cornucervi (Breda) Blume & Rchb.f. were cultured in VW (Vacin and Went, 1949) liquid medium added with chitosan from cuttlebone at five various concentrations of 5, 10, 15, 20 and 25 mg/l for 4 weeks. All chitosan concentrations could enhance protocorm production when compared to the control without chitosan. Additionally, protocorms were also cultured on VW semi-solid medium. The results showed the highest number of leaves (4.3 leaves), roots (2.5 roots) and shoots (1.5 shoots) could obtain when 15 mg/l of chitosan was added to the VW semi-solid medium. However, chitosan at higher concentration showed the inhibitory effect on the growth of protocorm. After the regenerated plantlets were potted in charcoal and watered with various concentrations of chitosan for 4 weeks, more than 50% were of them survived. Keywords : Chitosan, Phalaenopsis, Protocorm 1 Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani, Thailand 94000 * Corresponding Author, E-mail : psomporn@bunga.pn.psu.ac.th
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