Review

88 KKU Science Journal Volume 39 Number 1 Research
º≈°“√µ√«®«—¥°“√‡°‘¥ polymerization ´÷Ëß
‡ªìπ°“√‡ª≈’ˬπ “√µ—«°≈“ß (UV absorbing compound,
A 294
) ‡ªìπ “√ ’πÈ”µ“≈ (brown polymer, A 420
) · ¥ß
¥—ßµ“√“ß∑’Ë 1 ‚¥¬· ¥ß„π√Ÿª¢Õß 294/420 ratio
(Ajandouz et al., 2001) π—Èπ§◊ÕÀ“°§à“ 294/420 ratio
¡’§à“µË”· ¥ß∂÷ß°“√‡°‘¥ polymerization ¢Õß “√µ—«°≈“ß
(UV absorbing compound) ¡’§à“ Ÿß ®“°°“√∑¥≈Õß
§√—Èßπ’È “√ª√–°Õ∫‡‘ß´âÕπ¢Õ߉§‚µ·´π·≈–πÈ”µ“≈
°“·≈§‚µ (Chi+Gal) ·≈– “√ª√–°Õ∫‡‘ß´âÕπ¢Õß
‰§‚µ·´π·≈–πÈ”µ“≈Õ–√“∫‘‚π (Chi+Ara) ®–¡’°“√
‡°‘¥ polymerization Ÿß°«à“πÈ”µ“≈π‘¥Õ◊Ëπ
µ“√“ß∑’Ë 1 °“√‡ª≈’ˬπ “√µ—«°≈“ß (UV absorbing compound) ‡ªìπ “√ ’πÈ”µ“≈ (brown polymer) · ¥ß„π√Ÿª
¢Õß 294/420 ratio
π‘¥¢Õß “√ª√–°Õ∫‡‘ß´âÕπ
294/420 ratio
“√ª√–°Õ∫‡‘ß´âÕπ¢Õ߉§‚µ·´π·≈–πÈ”µ“≈ø√ÿ°‚µ (Chi+Fru) 21.49 + 0.62
“√ª√–°Õ∫‡‘ß´âÕπ¢Õ߉§‚µ·´π·≈–πÈ”µ“≈¡Õ≈‚µ (Chi+Mal) 27.08 + 0.72
“√ª√–°Õ∫‡‘ß´âÕπ¢Õ߉§‚µ·´π·≈–πÈ”µ“≈·≈§‚µ (Chi+Lac) 18.81 + 0.12
“√ª√–°Õ∫‡‘ß´âÕπ¢Õ߉§‚µ·´π·≈–πÈ”µ“≈°≈Ÿ‚§ (Chi+Glu) 18.62 + 0.15
“√ª√–°Õ∫‡‘ß´âÕπ¢Õ߉§‚µ·´π·≈–πÈ”µ“≈°“·≈§‚µ (Chi+Gal) 13.43 + 0.03
“√ª√–°Õ∫‡‘ß´âÕπ¢Õ߉§‚µ·´π·≈–πÈ”µ“≈Õ–√“∫‘‚π (Chi+Ara) 14.82 + 0.16
”À√—∫ªØ‘°‘√‘¬“‡¡≈≈“√å¥ (Maillard reaction,
nonenzymic browning reaction) π—È𠇪ìπªØ‘°‘√‘¬“¢Õß
reducing sugar °—∫ free amino group ·≈– maillard
browning product ®–‡°‘¥¢÷Èπ‡¡◊ËÕ„À⧫“¡√âÕπ “√
ª√–°Õ∫∑—Èß Õß∑’ËÕ¬Ÿà¥â«¬°—π (BeMiller and Whistler,
1996) §«“¡ —¡æ—π∏å√–À«à“ß 294/420 ratio ·≈–§à“
°“√¥Ÿ¥°≈◊π· ß∑’˧«“¡¬“«§≈◊Ëπ 420 π“‚π‡¡µ√ (´÷Ëß
‡ªìπ°“√«—¥°“√‡°‘¥ final stage ¢Õߪؑ°‘√‘¬“ ’πÈ”µ“≈)
æ∫«à“ r = - 0.771 ‚¥¬‡ªì𧫓¡ —¡æ—π∏å„π∑‘»∑“ß∑’Ë
µ√ߢⓡ°—π π—Èπ§◊Õ ‡¡◊ËÕ 294/420 ratio ¡’§à“µË” (´÷Ëß
· ¥ß∂÷ß°“√‡°‘¥ polymerization ∑’Ë¡“°) °“√‡°‘¥ “√
’πÈ”µ“≈∑’Ë final stage ®– Ÿß · ¥ß«à“°“√‡°‘¥ “√ ’
πÈ”µ“≈‡°‘¥®“° polymerization ´÷Ë߇ªìπ°“√‡ª≈’ˬπ “√
µ—«°≈“ß (UV absorbing compound) ‡ªìπ “√ ’πÈ”µ“≈
(brown polymer)
°“√»÷°…“§ÿ≥ ¡∫—µ‘„π°“√µâ“πÕπÿ¡Ÿ≈Õ‘ √–
1 °“√«—¥ Radical Scavenging Activity
‚¥¬„â DPPH Method
®“°°“√«—¥§ÿ≥ ¡∫—µ‘°“√µâ“πÕπÿ¡Ÿ≈Õ‘ √–
¢Õß “√ª√–°Õ∫‡‘ß´âÕπ¢Õ߉§‚µ·´π·≈–πÈ”µ“≈
π‘¥µà“ßÊ ‚¥¬°“√«—¥ radical scavenging activity
¥â«¬ DPPH Method ‡ª√’¬∫‡∑’¬∫°—∫πÈ”µ“≈‡¥’ˬ«Ê
·≈–‰§‚µ·´π‡¥’ˬ«Ê æ∫«à“ “√ª√–°Õ∫‡‘ß´âÕπ¢Õß
‰§‚µ·´π·≈–πÈ”µ“≈∑ÿ°π‘¥¡’§ÿ≥ ¡∫—µ‘„π°“√µâ“π
Õπÿ¡Ÿ≈Õ‘ √–«‘‡§√“–Àå‚¥¬«‘∏’ DPPH method Ÿß°«à“
πÈ”µ“≈·≈–‰§‚µ·´π‡¥’ˬ«Ê ¥—ß√Ÿª∑’Ë 4