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118 KKU Science Journal Volume 39 Number 1 Research<br />

In conclusion, chitosan could be prepared<br />

from cuttlebone and was applied successively<br />

to stimulate the growth and development of<br />

P. cornucervi (Breda) Blume & Rchb.f.<br />

Acknowledgement<br />

The authors are grateful to Asst. Prof.<br />

Dr. Vanida Chairgulprasert, Faculty of Science and<br />

Technology, Prince of Songkla University, for her<br />

kind proof-reading and comment of this manuscript<br />

and Mr. Jirasak Kongkoon for his help preparation<br />

of chitosan. This study was supported by grant from<br />

the Research Fund, Prince of Songkla University,<br />

Thailand.<br />

References<br />

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Economical Technique for Producing<br />

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G. (2004). Chitosan improve development,<br />

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Paphiopedilum bellatulum x PAPH. angthong<br />

derived from tissue culture. In the National<br />

Chitin-Chitosan Conference July 17-18. 2003.<br />

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University. Organized by Center for<br />

Chitin-Chitosan Biomaterials (CCB).<br />

Chulalongkorn University. pp. 65-68.<br />

Limpanavech, P., Pichyangkura, R., Khumwasi, C.,<br />

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R., Chaidee, A. and Akaraeakpanya, T.<br />

(2003). The effects of polymer type,<br />

concentation and $DD of biocatalyte<br />

modified Chitosan on floral production<br />

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60-64.<br />

Martino, A. D., Sittinger, M. and Risbud, M. V.<br />

(2005). Chitin: A versatile biopolymer for

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