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118 KKU Science Journal Volume 39 Number 1 Research In conclusion, chitosan could be prepared from cuttlebone and was applied successively to stimulate the growth and development of P. cornucervi (Breda) Blume & Rchb.f. Acknowledgement The authors are grateful to Asst. Prof. Dr. Vanida Chairgulprasert, Faculty of Science and Technology, Prince of Songkla University, for her kind proof-reading and comment of this manuscript and Mr. Jirasak Kongkoon for his help preparation of chitosan. This study was supported by grant from the Research Fund, Prince of Songkla University, Thailand. References Alimunair, A. and Zainuddin, R. (1992). An Economical Technique for Producing Chitosan Advances in Chitin and Chitosan. Brine, C. J., Sandford, P. A. Zikakis, J. P. (Ed). Elsevier Applied Science. Essex, UK. pp. 627-632. Barka, E. A., Eullaffroy, P., Clement, C. and Vernet, G. (2004). Chitosan improve development, and protects Vitis vinifera L. against Botrytis cinerea. Plant Cell Rep. 22: 608-614. Baxter, A., Dillon, M., Taylor, K. D. A. and Roberts, G. A. F. (1992). Improved method for i.r. determination of the degree of N-acetylation of chitosan. J. Biol. Macromol. 14: 166-169. Devlieghere, F., Vermeulen, A. and Debevere, J. (2004). Chitosan : antimicrobial activity, interactions with food components and applicability as a coating on fruit and vegetables. Food Microbiol. 26(6): 703-714. Hadwiger, L. A., Kloserman, S. J. and Choi, J. J. (2002). The Mode of Action of Chitosan and Its Oligomer in Inducing Plant Promoters and Developing Disease Resistance in Plants. (Suchiva, K., Chandrkrachang, S., Methacanon., Peter, M.G., eds.). Advances in Chitosan Science ISBN, Bangkok: 452-457. Kananont, N., Pichyangkura, R., Chanprame, S., Chadchawan, S. and Limpanavech, P. (2010). Chitosan specificity for the in vitro seed germination of two Dendrobium orchids (Asparagales: Orchidaceae). Scientia Horticulturae. 124: 239-247. Kleangkeo, C., Chankrajang, S. and Sawetsila, P. (2003). A study on the influences of chitosan upon the transplanting on growth of Paphiopedilum bellatulum x PAPH. angthong derived from tissue culture. In the National Chitin-Chitosan Conference July 17-18. 2003. at Institue Building III. Chulalongkorn University. Organized by Center for Chitin-Chitosan Biomaterials (CCB). Chulalongkorn University. pp. 65-68. Limpanavech, P., Pichyangkura, R., Khumwasi, C., Chadchawan, S., Lotrakul, P., Bunjongrat, R., Chaidee, A. and Akaraeakpanya, T. (2003). The effects of polymer type, concentation and $DD of biocatalyte modified Chitosan on floral production of Dendrobium ? Eiskol ?. In the National-Chitin-chitosan Conference. Bangkok. Thailand. July 17-18, 2003. pp. 60-64. Martino, A. D., Sittinger, M. and Risbud, M. V. (2005). Chitin: A versatile biopolymer for
ß“π«‘®—¬ «“√ “√«‘∑¬“»“ µ√å ¡¢. ªï∑’Ë 39 ©∫—∫∑’Ë 1 119 orthopaedic tissue-engineering. Biomaterials. 26: 5983-5990. Nge, K. L., New, N., Chandrkrachang, S. and Stevens, W. F. (2006). Chitosan as a growth stimulator in orchid tissue culture. Plant Sci. 170: 1185-1190. Pornpienpakdee, P., Singhasurasak, R., Chaiyasap, P., Pichyangkura, R., Bunjongrat, R., Chadchawan, S. and Limpanavech, P. (2010). Improving the micropropagation efficiency of hybrid Dendrobium orchids with chitosan. Scientia Horticulturae. 124: 490-499. Tajik, H, Moradi, M., Rohani, S. M. R., Erfani, A. M. and Jalali, F. S. S. (2008). Preparation of chitosan from brine shrimp (Artemia urmiana) cyst shells and effects of different chemical processing sequences on the physiochemical and functional properties of the product. Molecules. 13: 1263-1274. Vacin, E. and Went, F. (1949). Some pH change in nutrient solution. Bot. Gaz. 110: 605-613. Young, P. S., Murthy, H. N. and Yoeup, P. K. (2000). Mass multiplication of protocormlike bodies using bioreactor system and subsequent plant regeneration in Phalaenopsis. Plant Cell Tiss. Org. Cult. 63: 67-72. ❏❏❏❏❏
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