Review

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«“√ “√«‘∑¬“»“ µ√å ¡¢. ªï∑’Ë 39 ©∫—∫∑’Ë 1 21
ºŸâ‡¢’¬π¬—߉¥â∑”°“√»÷°…“°“√ àß∂à“¬¬’π‚¥¬„â
A. tumefaciens ·≈–ª√– ∫§«“¡ ”‡√Á®„πæ◊Õ—≠—π
´÷Ë߇ªìπæ◊„∫‡≈’ȬߧŸàº≈°“√»÷°…“ àß∂à“¬¬’π DFR ‡¢â“ Ÿà
Õ—≠—π À≈—ß®“° co-cultivation 4 ‡¥◊Õπ π”‡π◊ÈÕ‡¬◊ËÕÕ—≠—π
¡“∑”°“√µ√«® Õ∫‚¥¬‡∑§π‘§ GUS histochemical
assay (GUS expression) æ∫«à“‡°‘¥®ÿ¥ ’πÈ”‡ß‘π
(√Ÿª∑’Ë 1) ∑’ˇ°‘¥®“°ªØ‘°‘√‘¬“√–À«à“ß X-gluc ·≈– ≥
§‘¥‡ªìπ√âÕ¬≈– 20 ·≈–µ√«® Õ∫¥â«¬‡∑§π‘§ PCR
æ∫·∂∫¥’‡ÕÁπ‡Õ®“°ªØ‘°‘√‘¬“ PCR ”À√—∫¬’π GUS
∑ÿ°µâπ·≈–‰¥â·∂∫¥’‡ÕÁπ‡Õ∑’Ë¡’πÈ”Àπ—°‚¡‡≈°ÿ≈ª√–¡“≥
510 (√Ÿª∑’Ë 2) (Buddharak et al., 2009)
(°) (¢)
√Ÿª∑’Ë 1 GUS expression ¢ÕßÕ—≠—≠ Õ“¬ÿ 4 ‡¥◊Õπ À≈—ß co-cultivation ‚¥¬„â A. tumefasiens “¬æ—π∏ÿå
AGLO (°) Õ—≠—≠∑’ˉ¡à‰¥â àß∂à“¬¬’π (control) (¢) Õ—≠—≠∑’ˉ¥â√—∫°“√ àß∂à“¬ AGLO/ pBI121- DFR
(∑’Ë¡“: Buddharak et al., 2009)
√Ÿª∑’Ë 2 º≈¢Õߪؑ°‘√‘¬“ PCR ”À√—∫¬’π GUS
M : Lamda / PstI (marker)
NC : Õ—≠—≠∑’ˉ¡à‰¥â àß∂à“¬¬’π (control)
PC : æ≈“ ¡‘¥ pBI121
Lane1-9 : Õ—≠—≠ àß∂à“¬¬’π (AGLO/pBI121/DFR) (∑’Ë¡“: Buddharak et al., 2009)