Review

90 KKU Science Journal Volume 39 Number 1 Research
µ“√“ß∑’Ë 2 · ¥ß§à“ IC 50
·≈–§à“ Antiradical
Efficiency (AE)¢Õ߉§‚µ·´π·≈–πÈ”µ“≈‡¥’ˬ«Ê ´÷Ëß
æ∫«à“‰§‚µ·´π¡’§ÿ≥ ¡∫—µ‘„π°“√µâ“πÕπÿ¡Ÿ≈ DPPH
Ÿß°«à“πÈ”µ“≈∑ÿ°π‘¥ à«πµ“√“ß∑’Ë 3 · ¥ß§à“ IC 50
·≈–§à“ Antiradical Efficiency ¢Õß “√ª√–°Õ∫‡‘ß
´âÕπ¢Õ߉§‚µ·´π·≈–πÈ”µ“≈π‘¥µà“ßÊ º≈°“√
∑¥≈Õßæ∫«à“ “√ª√–°Õ∫‡‘ß´âÕπ¢Õ߉§‚µ·´π
·≈–πÈ”µ“≈Õ–√“∫‘‚π ¡’§ÿ≥ ¡∫—µ‘„π°“√µâ“πÕπÿ¡Ÿ≈
DPPH Ÿß°«à“ “√ª√–°Õ∫‡‘ß´âÕππ‘¥Õ◊ËπÊ ·µà‰¡à
·µ°µà“ßÕ¬à“ß¡’π—¬ ”§—≠∑“ß ∂‘µ‘°—∫ “√ª√–°Õ∫‡‘ß
´âÕπ¢Õ߉§‚µ·´π°—∫πÈ”µ“≈°“·≈§‚µ (p > 0.5)
‡¡◊ËÕæ‘®“√≥“§à“ IC 50
·µà·µ°µà“ß∑“ß ∂‘µ‘Õ¬à“ß¡’π—¬ ”§—≠
(p< 0.5) ‡¡◊ËÕæ‘®“√≥“§à“ AE º≈°“√∑¥≈Õߥ—ß°≈à“«
Õ¥§≈âÕß°—∫°“√∑¥≈ÕߢÕß Benjakul et al. (2005)
´÷Ëß∑¥≈Õß„π√–∫∫∑’Ë¡’‚ª√µ’π®“°æ≈“ ¡“À¡Ÿ·≈–
πÈ”µ“≈ ‚¥¬º≈°“√∑¥≈Õßæ∫«à“ §ÿ≥ ¡∫—µ‘„π°“√
µâ“πÕπÿ¡Ÿ≈Õ‘ √–¢Õß maillard reaction product ®“°
πÈ”µ“≈°“·≈§‚µ ®– Ÿß°«à“πÈ”µ“≈°≈Ÿ‚§ ·≈–ø√ÿ°‚µ
‡¡◊ËÕ«—¥¥â«¬«‘∏’ DPPH test ·≈–°“√«—¥ reducing power
”À√—∫°“√‡ª≈’ˬπ·ª≈ߧÿ≥ ¡∫—µ‘„π°“√µâ“π
Õπÿ¡Ÿ≈Õ‘ √–¢Õß “√ª√–°Õ∫‡‘ß´âÕπ∑’Ë Ÿß¢÷ÈπÕ“®‡ªìπ
‰ª„π·π«∑“߇¥’¬«°—∫°“√»÷°…“¢Õß Guérara and
Sumaya-Martinez (2003) ´÷Ëß»÷°…“„π√–∫∫¢Õß‚ª√µ’π
‰Œ‚¥√‰≈‡ µ∑”ªØ‘°‘√‘¬“°—∫πÈ”µ“≈°≈Ÿ‚§ º≈°“√»÷°…“
chromatographic profile °àÕπ·≈–À≈—ß°“√‡°‘¥
ªØ‘°‘√‘¬“‡¡≈≈“√å¥æ∫«à“ °“√‡°‘¥°“√®—¥‡√’¬ßµ—«°—π„À¡à
¢Õß‚¡‡≈°ÿ≈Õ“®‡ªìπµ—«à«¬„π°“√ª√—∫ª√ÿߧÿ≥ ¡∫—µ‘
„π°“√µâ“πÕπÿ¡Ÿ≈Õ‘ √–„À⥒¢÷Èπ
µ—«Õ¬à“ß√–∫∫µà“ßÊ ∑’Ë¡’°“√»÷°…“ “√ ’πÈ”µ“≈
®“°ªØ‘°‘√‘¬“‡¡≈≈“√å¥ (maillard reaction products,
MRP) µàÕ§ÿ≥ ¡∫—µ‘„π°“√µâ“πÕπÿ¡Ÿ≈Õ‘ √– ‡àπ
√–∫∫¢Õß°≈Ÿ‚§ -‰°≈´’π (Yoshimura et al., 1997)
√–∫∫¢Õß Ovalbumin ·≈– D-Aldohexoses (Sun et
al., 2006) ·≈–√–∫∫¢Õß coffee brews (Cämmerer
and Kroh, 2006) ‡ªìπµâπ
°“√ª√–¬ÿ°µå„â MRP ¡’°“√ª√–¬ÿ°µå„â„π
º≈‘µ¿—≥±åµà“ßÊ ‡àπ °“√ª√–¬ÿ°µå„â„π°“√¬—∫¬—Èß
‡Õπ‰´¡å polyphenoloxidase (PPO) „π°ÿâß°ÿ≈“¥”
(Matmaroh et al., 2006) °“√ª√–¬ÿ°µå„â„πº≈‘µ¿—≥±å
ª≈“´“√奒π (Tanaka et al., 1988) ·≈–°“√ª√–¬ÿ°µå
„â„πº≈‘µ¿—≥±å∫—µ‡µÕ√å§ÿ°°’È (Bressa et al., 1996)
‡ªìπµâπ
µ“√“ß∑’Ë 2 §à“ IC 50
·≈–§à“ Antiradical Efficiency ¢Õß “√≈–≈“¬‰§‚µ·´π·≈–πÈ”µ“≈‡¥’ˬ«Ê
π‘¥¢Õß “√
IC 50
(g/ml)
Antiradical Efficiency
πÈ”µ“≈¡Õ≈‚µ (Mal) 19571.56 a 0.00005 a
πÈ”µ“≈°≈Ÿ‚§ (Glu) 13487.11 b 0.00007 a
πÈ”µ“≈·≈§‚µ (Lac) 7604.60 c 0.00013 ab
πÈ”µ“≈°“·≈§‚µ (Gla) 4844.83 cd 0.00021 ab
πÈ”µ“≈ø√ÿ°‚µ (Fru) 3710.59 cd 0.00027 b
πÈ”µ“≈Õ–√“∫‘‚π (Ara) 2531.34 d 0.00040 c
‰§‚µ·´π (Chi) 2102.12 d 0.00048 d
À¡“¬‡Àµÿ -IC 50
: §«“¡‡¢â¡¢âπ¢Õß “√≈–≈“¬ (g/ml) ∑’Ë “¡“√∂∑”„Àâ§à“ % radical scavenging activity ≈¥≈ß
√âÕ¬≈– 50
-Õ—°…√∑’Ë·µ°µà“ß°—π„π·π«µ—Èß · ¥ß§«“¡·µ°µà“ß°—πÕ¬à“ß¡’π—¬ ”§—≠∑“ß ∂‘µ‘ (p