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∫∑§«“¡<br />

«“√ “√«‘∑¬“»“ µ√å ¡¢. ªï∑’Ë 39 ©∫—∫∑’Ë 1 29<br />

«‘∏’¡“µ√∞“π∑’Ëπ”¡“„â„π°“√µ√«®À“§«“¡<br />

º‘¥ª°µ‘„πºŸâªÉ«¬¡–‡√ÁßÀ≈“¬π‘¥ ‰¥â·°à°“√µ—¥‘Èπ‡π◊ÈÕ∑’Ë<br />

¡’欓∏‘ ¿“殓°ºŸâªÉ«¬‡æ◊ËÕÀ“§«“¡º‘¥ª°µ‘π—ÈπÊ<br />

Õ¬à“߉√°Áµ“¡ «‘∏’°“√¥—ß°≈à“«‡ªìπ«‘∏’∑’ˉ¡à‡À¡“–®–„â<br />

„π°“√µ√«®À“§«“¡º‘¥ª°µ‘„π√–¬–·√° √«¡∑—Èß°“√<br />

‡°Á∫‘Èπ‡π◊ÈÕµâÕßÕ“»—¬ºŸâ”π“≠·≈–Õ“® √â“ߧ«“¡<br />

‰¡à –¥«°·°àºŸâªÉ«¬ „π°“√«‘‡§√“–ÀåÀ“‚ª√µ’π®“° ‘Ëß<br />

àßµ√«®µà“ßÊ ‡àπ ´’√—¡·≈–ªí “«– ´÷Ë߇ªìπµ—«Õ¬à“ß<br />

∑’Ë¡’§«“¡‡À¡“– ¡ ‡π◊ËÕß®“° “¡“√∂‡®“–·≈–‡°Á∫‰¥â<br />

ßà“¬ (Luque-Garcia et al., 2007; Zhang et al., 2007)<br />

”À√—∫ªí≠À“¢Õß°“√π”´’√—¡¡“„â„π°“√<br />

»÷°…“‡æ◊ËÕ§âπÀ“ “√∫àß’È∑“ß’«¿“æ∑’Ë¡’§«“¡®”‡æ“–<br />

π—Èπ§◊Õ¡’°“√∫¥∫—ߢÕß‚ª√µ’π∑’Ë¡’ª√‘¡“≥¡“° (high<br />

abundance proteins) ‚¥¬°«à“√âÕ¬≈– 95 ¢Õß‚ª√µ’π<br />

∑—ÈßÀ¡¥ ‡ªìπ Õ—≈∫Ÿ¡‘π (albumin), Õ‘¡¡Ÿ‚π°≈Õ∫∫Ÿ≈‘π<br />

(immunoglobulins), ∑√“π ‡øÕ√‘π (transferin),<br />

·Õπµ‘∑√‘ª´‘π (α-1-antitrypsin), ·Œª‚µ°≈Õ∫∫Ÿ≈‘π<br />

(haptoglobulin) ‡ªìπµâπ ‚¥¬‚ª√µ’π∑’Ë¡’ª√‘¡“≥¡“°<br />

‡À≈à“π’È®–∫¥∫—ß‚ª√µ’π∑’Ë¡’ª√‘¡“≥πâÕ¬ (low abundance<br />

proteins) ´÷Ëß¡—°‡ªìπ°≈ÿà¡‚ª√µ’π∑’Ë¡’§«“¡ ”§—≠∑“ß<br />

§≈‘π‘° (Anderson, 2005; Xue et al., 2010)<br />

°“√‡µ√’¬¡µ—«Õ¬à“ß°àÕπ°“√µ√«®«‘‡§√“–Àå<br />

®÷ß¡’§«“¡ ”§—≠Õ¬à“߬‘Ëß∑’Ë®–૬≈¥¢âÕ®”°—¥¥—ß°≈à“«<br />

‰¥â ‚¥¬À≈—°°“√Õ¬à“ßßà“¬‡ªìπ·∫∫ affinity ´÷Ëß “¡“√∂<br />

∑”‰¥â Õߪ√–°“√ ”À√—∫ª√–°“√·√° §◊Õ °“√·¬°<br />

‡Õ“‚ª√µ’π∑’Ë¡’ª√‘¡“≥¡“°ÕÕ°®“°µ—«Õ¬à“ß (removal<br />

techniques) (Ahmed et al., 2003; Bjorhall et al.,<br />

2005; Zolotarjova et al., 2005) ª√–°“√∑’Ë Õß<br />

§◊Õ°“√·¬àß®—∫¢Õß‚ª√µ’π∑’˵âÕß°“√ (enrichment<br />

techniques) (Boschetti et al., 2008; Keidel et al.,<br />

2010) ∑—Èß Õß«‘∏’ “¡“√∂‡æ‘Ë¡ª√– ‘∑∏‘¿“æ°“√µ√«®<br />

«‘‡§√“–Àå¢Õß‚ª√µ’π∑’Ë¡’ª√‘¡“≥πâÕ¬ ´÷Ëß𔉪 Ÿà°“√<br />

‡æ‘Ë¡‚Õ°“ „π°“√§âπæ∫‚ª√µ’π∑’Ë¡’§«“¡ ”§—≠∑“ß<br />

§≈‘π‘° „π∑’Ëπ’È¢Õ¬°µ—«Õ¬à“߇∑§π‘§Õ¬à“ßßà“¬∑’Ë “¡“√∂<br />

∑”‰¥â„πÀâÕߪؑ∫—µ‘°“√∑’ˉ¡àµâÕß°“√‡§√◊ËÕß¡◊Õ摇»…<br />

‰¥â·°à °“√„â cibacron blue gels ·≈– immunoaffinity<br />

depletion method (Bodzon-Kulakowska et al., 2007)<br />

‚¥¬∑—Èß Õ߇∑§π‘§π’È®–‡ªìπ°“√·¬°‡Õ“‚ª√µ’π∑’Ë¡’<br />

ª√‘¡“≥¡“°ÕÕ°®“°µ—«Õ¬à“ß ´÷Ë߇ªìπ«‘∏’¥—È߇¥‘¡∑’Ë¡’°“√<br />

„âÕ¬à“ß°«â“ߢ«“ß„πÀâÕߪؑ∫—µ‘°“√ „πªí®®ÿ∫—π‰¥â¡’<br />

°“√æ—≤π“‡∑§π‘§„À¡à¢÷Èπ¡“‡√’¬°«à“ equalizer bead<br />

technology ´÷Ë߇ªìπ°“√·¬àß®—∫¢Õß‚ª√µ’π∑’˵âÕß°“√ ‡ªìπ<br />

«‘∏’„À¡à®÷߬—߉¡à·æ√àÀ≈“¬¡“°π—° (Garbis et al., 2005)<br />

«‘∏’π’È®– “¡“√∂≈¥¢âÕ®”°—¥¢Õß«‘∏’°“√¥—È߇¥‘¡ ‚¥¬®–<br />

‰¥â°≈à“«√“¬≈–‡Õ’¬¥À≈—°°“√¢Õß·µà≈–«‘∏’ √«¡∑—Èß<br />

¢âÕ¥’·≈–¢âÕ®”°—¥ ·≈–°“√ª√–¬ÿ°µå°“√„âß“πµàÕ‰ª<br />

°“√·¬°‡Õ“‚ª√µ’π∑’Ë¡’ª√‘¡“≥¡“°ÕÕ°®“°<br />

µ—«Õ¬à“ß (removal techniques)<br />

1. °“√„â cibacron blue gels<br />

Õ—≈∫Ÿ¡‘π®—∫°—∫ cibacron blue gels ‰¥â‚¥¬<br />

Õ“»—¬‚§√ß √â“ß∑’Ë¡’≈—°…≥–°“√µàÕ°—π‡ªìπ«ß·À«π<br />

∑’Ë∂Ÿ°¬÷¥µ‘¥Õ¬Ÿà∫πº‘«¢Õ߇¡Á¥æ≈“ µ‘° (bead) ¥â«¬<br />

æ—π∏–‚§«“‡≈πµå ‡√’¬°‚§√ß √â“ßπ’È«à“ cibacron blue<br />

gels (√Ÿª∑’Ë 1) „π∏√√¡“µ‘Õ—≈∫Ÿ¡‘π®–∑”Àπâ“∑’ˇªìπµ—«æ“<br />

‚¥¬®–®—∫°—∫‚ª√µ’π‰¥âÀ≈“¬π‘¥ ¥—ßπ—Èπ cibacron blue<br />

gels ®÷߉¥â¡’°“√ÕÕ°·∫∫µ“¡‚§√ß √â“ß∑’Ëæ∫„π<br />

∏√√¡“µ‘‡æ◊ËÕ„â„π°“√®—∫°—∫Õ—≈∫Ÿ¡‘π ¥â«¬æ—π∏–<br />

Õ¬à“ßÕàÕπ

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