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86 KKU Science Journal Volume 39 Number 1 Research (a) (b) Absorbance (420 nm) 0.04 0.04 0.03 0.03 0.02 0.02 0.01 0.01 0.00 Lac Chi Chi+Lac Absorbance (420 nm) 0.03 0.02 0.02 0.01 0.01 0.00 Mal Chi Chi+Mal -0.01 0.5 1.0 1.5 2.0 2.5 Concentration (%) -0.01 0.5 1.0 1.5 2.0 2.5 Concentration (%) (c) (d) Absorbance (420 nm) 0.010 0.008 0.006 0.004 0.002 0.000 Fru Chi Chi+Fru Absorbance (420 nm) 0.25 0.20 0.15 0.10 0.05 0.00 Ara Chi Chi+Ara -0.002 0.5 1.0 1.5 2.0 2.5 Concentration (%) -0.05 0.5 1.0 1.5 2.0 2.5 Concention (%) (e) (f) Absorbance (420 nm) 0.14 0.12 0.10 0.08 0.06 0.04 0.02 0.00 Gal Chi Chi+Gal Absorbance (420 nm) 0.11 0.09 0.07 0.05 0.03 0.01 Glu Chi Chi+Glu -0.02 0.5 1.0 1.5 2.0 2.5 Concentration (% ) -0.01 0.5 1.0 1.5 2.0 2.5 Concentration (%) √Ÿª∑’Ë 2 §à“°“√¥Ÿ¥°≈◊π· ß∑’Ë§«“¡‡¢â¡¢âπµà“ßÊ°—π ®“°ªØ‘°‘√‘¬“‡¡≈≈“√å¥∑’Ë final state (A 420 ) ¢Õß “√ª√–°Õ∫ ‡‘ß´âÕπ√–À«à“ß‰§‚µ·´π·≈–πÈ”µ“≈ (a : lactose, b: maltose, c: fructose, d: arabinose, e: galactose and f: glucose) ‡ª√’¬∫‡∑’¬∫°—∫πÈ”µ“≈‡¥’Ë¬«Ê ·≈–‰§‚µ·´π‡¥’Ë¬«Ê (Chi)
ß“π«‘®—¬ «“√ “√«‘∑¬“»“ µ√å ¡¢. ªï∑’Ë 39 ©∫—∫∑’Ë 1 87 Absorbance (294 nm) 3.5 3 2.5 2 1.5 1 Chi+Fru Chi+Mal Chi+Lac Chi+Glu Chi+Gla Chi+Ara 0.5 0 0.5 1.0 1.5 2.0 2.5 Concentration (%) (b) Absorbance (420 nm) 0.25 0.2 0.15 0.1 0.05 Chi+Fru Chi+Mal Chi+Lac Chi+Glu Chi+Gal Chi+Ara 0 0.5 1.0 1.5 2.0 2.5 Concentration (%) √Ÿª∑’Ë 3 §à“°“√¥Ÿ¥°≈◊π· ß∑’Ë§«“¡‡¢â¡¢âπµà“ßÊ °—π ®“°ªØ‘°‘√‘¬“‡¡≈≈“√å¥∑’Ë final state (a) ·≈– intermediate state (b) ¢Õß “√ª√–°Õ∫‡‘ß´âÕπ√–À«à“ß‰§‚µ·´π·≈–πÈ”µ“≈π‘¥µà“ßÊ
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