Review

«.«‘∑¬. ¡¢. 39(1) 28-35 (2554) KKU Sci. J.39(1) 28-35 (2011)
°“√‡µ√’¬¡´’√—¡¥â«¬‡∑§π‘§Õ¬à“ßßà“¬‡æ◊ËÕ°“√§âπæ∫
“√∫àß’È∑“ß’«¿“æ
Simple Techniques for Serum Preparations
in Biomarkers Discovery
»‘√‘æ√ ¿—∑√°‘®°”®√ 1,2* ¡π∑‘√“ ®–π—π∑å 3
∫∑π”
ªí®®ÿ∫—π°“√»÷°…“‡°’ˬ«°—∫‚ª√µ’π¥â«¬‡∑§π‘§
∑“ß‚ª√µ‘‚Õ¡‘§ å (proteomics) ‰¥â‡¢â“¡“¡’∫∑∫“∑
Õ¬à“ß¡“°„π°“√§âπÀ“ “√∫àß’È∑“ß’«¿“æ (biomarkers)
√«¡∑—ÈßÕ“®π”‰ª Ÿà°“√§âπæ∫‡ªÑ“À¡“¬ (targets) „π
°“√ÕÕ°·∫∫‡°’ˬ«°—∫¬“√—°…“‚√§ (Garbis et al.,2005)
∑—Èßπ’ȇ∑§π‘§∑“ß‚ª√µ‘‚Õ¡‘§ å Õ“∑‘ two dimensional
gel electrophoresis (2DE) ·≈–·¡ ‡ª°‚∑√‡¡∑√’
(mass spectrometry) ‡ªìπ‡∑§π‘§∑’Ë¡’§«“¡‰« Ÿß„π
°“√«‘‡§√“–À傪√µ’π‰¥â√–¥—∫π“‚π‚¡≈∂÷߇ø¡‚µ‚¡≈
(Kenny et al.,1992; Gatlin et al., 1998) ∑—È߬—߉¥â¡’
°“√æ—≤π“ª√– ‘∑∏‘¿“æÕ¬à“ßµàÕ‡π◊ËÕß ¥—ßπ—Èπ¥â«¬‡∑§π‘§
∑’Ë¡’ª√– ‘∑∏‘¿“æ Ÿßπ’Èπà“®–𔉪 Ÿà°“√§âπæ∫ “√∫àß’È
∑“ß’«¿“æ ´÷Ëß®–‡ªìπª√–‚¬πåµàÕß“π∑“ߥâ“π°“√
·æ∑¬å¡“°¬‘Ëߢ÷Èπ (Aebersold et al., 2003; Khan et al.,
2005)
°“√§âπæ∫‚ª√µ’π∑’ˇªìπ “√∫àß’È∑“ß’«¿“æ
¡’∫∑∫“∑„π°“√ªÑÕß°—π °“√«‘π‘®©—¬‚√§ °“√µ‘¥µ“¡
Õ“°“√¢ÕߺŸâªÉ«¬‰¥â √«¡∑—Èß√–¥—∫¢Õß “√∫àß’È∑“ß
’«¿“æ∑’˵√«®æ∫„π·µà≈–√–¬–π—Èπ¬—ß “¡“√∂‡ªìπ
¢âÕ¡Ÿ≈„π°“√«“ß·ºπ°“√√—°…“‰¥âÕ’°¥â«¬ „πªí®®ÿ∫—π
¡’°“√π” “√∫àß’È¡–‡√Áß (tumor markers) À≈“¬π‘¥
¡“„â„πß“π∑“ߧ≈‘π‘° ´÷Ëß “√∫àß’È¡–‡√Áß„π·µà≈–π‘¥
®–¡’§«“¡®”‡æ“–µàÕ¡–‡√Áß∑’Ë·µ°µà“ß°—π µ—«Õ¬à“߇àπ
“√æ√Õ ‡µ∑ ‡ª§´‘øÕ𵑇®π (prostate specific
antigen, PSA) „π´’√—¡®–„â∫àß’È¡–‡√ÁßµàÕ¡≈Ÿ°À¡“°·≈–
“√Õ—≈ø“ø‚ª√µ’π (alpha-fetoprotein, AFP) „π´’√—¡
®–„â∫àß’È¡–‡√Áßµ—∫ ‡ªìπµâπ Õ¬à“߉√°Á¥’ “√∫àߒȇÀ≈à“
π—Èπ¬—߉¡à‡À¡“– ¡∑“ߧ≈‘π‘°„π°“√µ√«®æ∫¡–‡√Áß„π
√–¬–·√°Ê ‡π◊ËÕß®“°¡’¢âÕ®”°—¥‡°’ˬ«°—∫§«“¡‰«·≈–
§«“¡®”‡æ“–„π°“√µ√«®«—¥ (Block et al., 2005;
Sardana et al., 2007; Sardana et al., 2008)
1
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®.¢Õπ·°àπ 40002
2
»Ÿπ¬å«‘®—¬æ¬“∏‘„∫‰¡â„πµ—∫·≈–¡–‡√Áß∑àÕπÈ”¥’ §≥–·æ∑¬»“ µ√å ¡À“«‘∑¬“≈—¬¢Õπ·°àπ Õ.‡¡◊Õß ®.¢Õπ·°àπ 40002
2
“¢“«‘“«‘∑¬“»“ µ√å°“√·æ∑¬å §≥–‡∑§π‘§°“√·æ∑¬å ¡À“«‘∑¬“≈—¬¢Õπ·°àπ Õ.‡¡◊Õß ®.¢Õπ·°àπ 40002
* Corresponding Author, E-mail: sirpat@kku.ac.th