Review

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Transplantation of plantlets
Plantlets transferred to charcoal (Table 3
and Figure 2) had no statistical difference among
given chitosan. However, about 77.78% of survival
could receive on plants developed in charcoal
and treated with 10 mg/l chitosan solution. The
regenerated plants were characterized by growth and
produced well of normal shoot. Our investigation
was similar to the previous report that 10 mg/l
chitosan stimulated shoot and root in Paphiopedium
bellatum x PAPH. Angthong (Kleangkeo et al., 2003).
Table 3. Effect of chitosan on the percentage of survival of P. cornucervi (Breda) Blume & Rchb.f. after
4 weeks of transplantation.
Concentration of chitosan
(mg/l)
survival rate (%) characteristic of plantlets
0 72.22 a leaves and roots were well developed
5 61.11a plantlet were stunted
10 77.78a multiplicated of shoot, leaves and roots
were well developed
15 70.00a leaves and roots were well developed
20 71.60a leaves and roots were well developed
25 60.00a leaves and roots were well developed
Figure 2.
Transplantation of plants on charcoal and watering with 10 mg/l chitosan after 4 weeks of
transfer.